Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension

2003 ◽  
pp. 97-105
Author(s):  
Michael J. Dunn
Keyword(s):  
Isoelectric Focusing ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Two Dimensional

scholarly journals A novel two-dimensional polyacrylamide-gel pattern, which may be due to allelic genes, of phenylalanine hydroxylase in monkeys

1985 ◽  
Vol 231 (1) ◽  
pp. 197-199 ◽  
Author(s):  
S C Smith ◽  
W McAdam ◽  
R G H Cotton ◽  
J F B Mercer
Keyword(s):  
Isoelectric Focusing ◽  
Gel Electrophoresis ◽  
Phenylalanine Hydroxylase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
The Novel ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Monkey Liver

Two-dimensional gel electrophoresis of immunopurified monkey liver phenylalanine hydroxylase showed a novel form of the enzyme, in 4 out of 24 monkeys, in which each polypeptide spot was split into a doublet with the same charge but slightly different mobility in the sodium dodecyl sulphate/polyacrylamide-gel-electrophoresis (as opposed to the isoelectric-focusing) dimension. Phenylalanine hydroxylase formed by translation of RNA from a liver containing the novel form showed the doublet pattern, suggesting that it is due to differences in mRNA. By analogy with the rat, this mRNA difference could be due to allelic genes.

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