Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification

2003 ◽  
pp. 79-98
Author(s):  
Wolfgang Klein
Keyword(s):  
Protein Purification ◽  
Affinity Tag ◽  
Binding Peptide ◽  
Calmodulin Binding

scholarly journals Several Affinity Tags Commonly Used in Chromatographic Purification

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Xinyu Zhao ◽  
Guoshun Li ◽  
Shufang Liang
Keyword(s):  
Protein Complexes ◽  
Factor Xa ◽  
Biological Research ◽  
Fusion Partner ◽  
Glutathione S Transferase ◽  
Affinity Tag ◽  
Affinity Tags ◽  
Binding Peptide ◽  
Calmodulin Binding ◽  
Large Size

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, Strep II tag, streptavidin-binding peptide (SBP) tag, calmodulin-binding peptide (CBP), glutathione S-transferase (GST), maltose-binding protein (MBP), S-tag, HA tag, and c-Myc tag. In some cases, a large-size affinity tag, such as GST or MBP, can significantly impact on the structure and biological activity of the fusion partner protein. So it is usually necessary to excise the tag by protease. The most commonly used endopeptidases are enterokinase, factor Xa, thrombin, tobacco etch virus, and human rhinovirus 3C protease. The proteolysis features of these proteases are described in order to provide a general guidance on the proteolytic removal of the affinity tags.

Affinity Tags for Protein Purification

2020 ◽  
Vol 21 (8) ◽  
pp. 821-830
Author(s):  
Vibhor Mishra
Keyword(s):  
Protein Purification ◽  
Protein Complexes ◽  
Affinity Purification ◽  
Protein Domain ◽  
Affinity Tag ◽  
Small Peptides ◽  
Affinity Tags ◽  
C Terminus ◽  
Solubility Enhancers ◽  
As Solubility

The affinity tags are unique proteins/peptides that are attached at the N- or C-terminus of the recombinant proteins. These tags help in protein purification. Additionally, some affinity tags also serve a dual purpose as solubility enhancers for challenging protein targets. By applying a combinatorial approach, carefully chosen affinity tags designed in tandem have proven to be very successful in the purification of single proteins or multi-protein complexes. In this mini-review, the key features of the most commonly used affinity tags are discussed. The affinity tags have been classified into two significant categories, epitope tags, and protein/domain tags. The epitope tags are generally small peptides with high affinity towards a chromatography resin. The protein/domain tags often perform double duty as solubility enhancers as well as aid in affinity purification. Finally, protease-based affinity tag removal strategies after purification are discussed.

scholarly journals A calmodulin-binding peptide of caldesmon.

1991 ◽  
Vol 266 (32) ◽  
pp. 21810-21814
Author(s):  
Q.Q. Zhan ◽  
S.S. Wong ◽  
C.L. Wang
Keyword(s):  
Binding Peptide ◽  
Calmodulin Binding

scholarly journals Characterization of a synthetic calmodulin-binding peptide derived from Bacillus anthracis adenylate cyclase.

1993 ◽  
Vol 268 (3) ◽  
pp. 1695-1701
Author(s):  
H. Munier ◽  
F.J. Blanco ◽  
B. Prêcheur ◽  
E. Diesis ◽  
J.L. Nieto ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Bacillus Anthracis ◽  
Binding Peptide ◽  
Calmodulin Binding

scholarly journals Recovery of Polypeptides Cleaved from Purified Calmodulin-Binding Peptide Fusion Proteins

BioTechniques ◽  
1997 ◽  
Vol 22 (3) ◽  
pp. 451-453 ◽  
Author(s):  
Peter Vaillancourt ◽  
Timothy G. Simcox ◽  
Chao-Feng Zheng
Keyword(s):  
Fusion Proteins ◽  
Binding Peptide ◽  
Calmodulin Binding

The model calmodulin-binding peptide melittin inhibits phosphorylase kinase by interacting with its catalytic center

Biochemistry ◽  
1993 ◽  
Vol 32 (44) ◽  
pp. 11865-11872 ◽  
Author(s):  
Hemant K. Paudel ◽  
Yi Hong Xu ◽  
Harry W. Jarrett ◽  
Gerald M. Carlson
Keyword(s):  
Phosphorylase Kinase ◽  
Catalytic Center ◽  
Binding Peptide ◽  
Calmodulin Binding

A luminescent affinity tag for proteins based on the terbium(III)-binding peptide

2012 ◽  
Vol 422 (1) ◽  
pp. 52-54 ◽  
Author(s):  
Shinji Sueda ◽  
Shogo Tanaka ◽  
Sayomi Inoue ◽  
Hideyuki Komatsu
Keyword(s):  
Affinity Tag ◽  
Binding Peptide
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