Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants

2008 ◽  
pp. 133-154 ◽  
Author(s):  
Rodney S. Nairn ◽  
Gerald M. Adair
Keyword(s):  
Dna Repair ◽  
Gene Targeting ◽  
Mammalian Cell ◽  
Dna Repair Mutants

scholarly journals The Gene Targeting Approach of Small Fragment Homologous Replacement (SFHR) Alters the Expression Patterns of DNA Repair and Cell Cycle Control Genes

2016 ◽  
Vol 5 ◽  
pp. e304 ◽  
Author(s):  
Silvia Pierandrei ◽  
Andrea Luchetti ◽  
Massimo Sanchez ◽  
Giuseppe Novelli ◽  
Federica Sangiuolo ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Repair ◽  
Gene Targeting ◽  
Cell Cycle Control ◽  
Expression Patterns ◽  
Small Fragment

A mammalian cell line deficient in activity of the DNA repair enzyme 5-hydroxymethyluracil-DNA glycosylase is resistant to the toxic effects of the thymidine analog 5-hydroxymethyl-2'-deoxyuridine

1992 ◽  
Vol 12 (12) ◽  
pp. 5536-5540
Author(s):  
R J Boorstein ◽  
L N Chiu ◽  
G W Teebor
Keyword(s):  
Dna Repair ◽  
Cell Line ◽  
Mammalian Cell ◽  
Dna Glycosylase ◽  
Mammalian Cell Line ◽  
Repair Enzyme ◽  
Thymidine Analog ◽  
Large Numbers ◽  
Mechanism Of Toxicity ◽  
Base Excision

We isolated a mutant mammalian cell line lacking activity for the DNA repair enzyme 5-hydroxymethyluracil-DNA glycosylase (HmUra-DNA glycosylase). The mutant was isolated through its resistance to the thymidine analog 5-hydroxymethyl-2'-deoxyuridine (HmdUrd). The mutant incorporates HmdUrd into DNA to the same extent as the parent line but, lacking the repair enzyme, does not remove it. The phenotype of the mutant demonstrates that the toxicity of HmdUrd does not result from substitution of thymine in DNA by HmUra but rather from the removal via base excision of large numbers of HmUra residues in DNA. This finding elucidates a novel mechanism of toxicity for a xenobiotic nucleoside. Furthermore, the isolation of this line supports our hypothesis that the enzymatic repairability of HmUra derives not from its formation opposite adenine via the oxidation of thymine, but rather from its formation opposite guanine as a product of the oxidation and subsequent deamination of 5-methylcytosine.

scholarly journals Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells.

1981 ◽  
Vol 78 (6) ◽  
pp. 3734-3737 ◽  
Author(s):  
L. H. Thompson ◽  
D. B. Busch ◽  
K. Brookman ◽  
C. L. Mooney ◽  
D. A. Glaser
Keyword(s):  
Genetic Diversity ◽  
Dna Repair ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Dna Repair Mutants

scholarly journals Conditional DNA repair mutants enable highly precise genome engineering

2014 ◽  
Vol 42 (8) ◽  
pp. e62-e62 ◽  
Author(s):  
Ákos Nyerges ◽  
Bálint Csörgő ◽  
István Nagy ◽  
Dóra Latinovics ◽  
Béla Szamecz ◽  
...  
Keyword(s):  
Dna Repair ◽  
Genome Engineering ◽  
Dna Repair Mutants
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