Detection and Quantitation of Circulating Plasmodium falciparum DNA by Polymerase Chain Reaction

2006 ◽  
pp. 155-162 ◽  
Author(s):  
Shira Gal ◽  
James S. Wainscoat
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodium Falciparum ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Missed Plasmodium falciparum and Plasmodium vivax Mixed Infections in Ethiopia Threaten Malaria Elimination

2021 ◽  
Author(s):  
Colleen M. Leonard ◽  
Hussein Mohammed ◽  
Mekonnen Tadesse ◽  
Jessica N. McCaffery ◽  
Doug Nace ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodium Falciparum ◽  
Lactate Dehydrogenase ◽  
Plasmodium Vivax ◽  
Therapeutic Efficacy ◽  
Dried Blood Spots ◽  
Mixed Infections ◽  
Efficacy Study ◽  
Chain Reaction ◽  
Polymerase Chain

Plasmodium falciparum and Plasmodium vivax are co-endemic in Ethiopia. This study investigated whether mixed infections were missed by microscopy from a 2017 therapeutic efficacy study at two health facilities in Ethiopia. All patients (N = 304) were initially classified as having single-species P. falciparum (n = 148 samples) or P. vivax infections (n = 156). Dried blood spots were tested for Plasmodium antigens by bead-based multiplex assay for pan-Plasmodium aldolase, pan-Plasmodium lactate dehydrogenase, P. vivax lactate dehydrogenase, and histidine-rich protein 2. Of 304 blood samples, 13 (4.3%) contained both P. falciparum and P. vivax antigens and were analyzed by polymerase chain reaction for species-specific DNA. Of these 13 samples, five were confirmed by polymerase chain reaction for P. falciparum/P. vivax co-infection. One sample, initially classified as P. vivax by microscopy, was found to only have Plasmodium ovale DNA. Plasmodium falciparum/P. vivax mixed infections can be missed by microscopy even in the context of a therapeutic efficacy study with multiple trained readers.

HRP2/3 mutation in recrudescent Plasmodium falciparum malaria case acquired in Ethiopia

2020 ◽  
Author(s):  
Shiraz Gefen-Halevi ◽  
Valentin Belinson ◽  
Uri Manor ◽  
Zeala Gazit ◽  
Gill Smollan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodium Falciparum ◽  
Lactate Dehydrogenase ◽  
Falciparum Malaria ◽  
Malaria Case ◽  
Rapid Detection ◽  
Plasmodium Falciparum Malaria ◽  
Malaria Prophylaxis ◽  
Chain Reaction ◽  
Polymerase Chain

A 65-year-old Israeli working in Welkait, Ethiopia, not using malaria prophylaxis, developed fever. Malaria rapid detection test was consistent with non-falciparum malaria (plasmodium lactate dehydrogenase+/histidine-rich protein− [LDH+/HRP−]) but microscopy showed typical Plasmodium falciparum. HRP2/3 were negative by polymerase chain reaction. The patient suffered two recrudescence episodes following artemether–lumefantrine and atovaquone–proguanil treatments, and responded to mefloquine treatment.

Evaluation of Imported Plasmodium falciparum Malaria Cases: The Use of Polymerase Chain Reaction in Diagnosis

2013 ◽  
Vol 47 (4) ◽  
pp. 668-676 ◽  
Author(s):  
Hayati DEMİRASLAN ◽  
Emrah ERDOĞAN ◽  
Zeynep TÜRE ◽  
Salih KUK ◽  
Süleyman YAZAR ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodium Falciparum ◽  
Falciparum Malaria ◽  
Plasmodium Falciparum Malaria ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of Plasmodium falciparum in Human Blood by a Nested Polymerase Chain Reaction

1994 ◽  
Vol 51 (5) ◽  
pp. 617-626 ◽  
Author(s):  
Meiji Arai ◽  
Yusuke Wataya ◽  
Toshifumi Kakutani ◽  
Chiyoko Mizukoshi ◽  
Fumie Kubochi
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodium Falciparum ◽  
Human Blood ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain
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