Soybean (Glycine max) Transformation Using Mature Cotyledonary Node Explants

2006 ◽  
pp. 385-396 ◽  
Author(s):  
Paula M. Olhoft ◽  
Christopher M. Donovan ◽  
David A. Somers
Keyword(s):  
Glycine Max ◽  
Cotyledonary Node ◽  
Cotyledonary Node Explants

Assessing the effect of phytohormone on growth and germination of soybean (Glycine max (L.) Merr.) from cotyledonary node

2018 ◽  
Vol 51 (1) ◽  
Author(s):  
Lubna Faraz ◽  
Muhammad Faheem Siddiqui ◽  
Saddia Galani ◽  
Faisal Mehdi
Keyword(s):  
Glycine Max ◽  
Cotyledonary Node ◽  
Glycine Max L

EFFECT OF SINGLE-WALL CARBON NANOTUBE ON SOYBEAN (GLYCINE MAX) REGENERATION FROM MATURE COTYLEDONARY NODE EXPLANTS

Nano LIFE ◽  
2012 ◽  
Vol 02 (04) ◽  
pp. 1250014 ◽  
Author(s):  
X. H. WANG ◽  
Q. M. HUANG ◽  
L. WANG ◽  
L. Z. WANG
Keyword(s):  
Carbon Nanotube ◽  
Adventitious Shoots ◽  
Cotyledonary Node ◽  
Shoot Elongation ◽  
Regeneration Frequency ◽  
Single Wall Carbon Nanotube ◽  
Single Wall Carbon ◽  
Plantlet Formation ◽  
Shoot Initiation ◽  
Cotyledonary Node Explants

One of the major limitations in producing transgenic soybeans using the agrobacterium-mediated cotyledonary-node method is low regeneration frequency. An improved highly efficient regeneration system of soybean was established herein. Cotyledonary node explants were placed in shoot initiation medium with single-wall carbon nanotube (SWNT) for adventitious shoots regeneration, and adventitious shoots were subcultured in shoot elongation medium with SWNT for shoot elongation and rooting. 40 mg/L SWNT supplemented in shoot initiation medium was found to be the optimal concentration with shoots regeneration frequency significantly increased by 21.5% compared with the control treatment, while for 4 mg/L and 400 mg/L, the increase was 4.6% and 6.5%, respectively. Faster elongation and rooting of adventitious shoots was observed in shoot elongation medium with 40 mg/L SWNT. Soybean plantlet formation frequency within the limited four weeks supplemented with 40 mg/L SWNT reached 48.4% while in the other three treatments: 4 mg/L, 400 mg/L and control, 0% were observed. These results indicate that supplement of SWNT in the soybean medium can efficiently promote adventitious shoots formation frequency, increase plantlet formation frequency and shorten the regeneration period.

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