Chebulinic Acid Suppresses Adipogenesis in 3T3-L1 Preadipocytes by Inhibiting PPP1CB Activity

Depletion of protein phosphatase-1 catalytic subunit beta (PPP1CB), a serine/threonine protein phosphatase and potent adipogenic activator, suppresses the differentiation of 3T3-L1 preadipocytes into mature adipocytes. Therefore, PPP1CB is considered as a potential therapeutic target for obesity. We screened 1033 natural products for PPP1CB inhibitors and identified chebulinic acid, which is abundantly present in the seeds of Euphoria longana and fruits of Terminalia chebula. Chebulinic acid strongly inhibited the hydrolysis of 6,8-difluoro-4-methylumbelliferyl phosphate by PPP1CB (IC50 = 300 nM) and demonstrated potent antiadipogenic effects in 3T3-L1 preadipocytes in a concentration-dependent manner. Additional studies have demonstrated that chebulinic acid suppresses early differentiation by downregulating key transcription factors that control adipogenesis in 3T3-L1 cells. These results suggested that chebulinic acid may be a potential therapeutic agent for treating obesity by inhibiting PPP1CB activity.

Crude Extract of Terminalia Chebula Fruit Effect on Gastro Intestinal Disorders Using Different Animal Models

Terminalia chebula is an ancient medicinal herb. it is also known as Haritaki, Yellow myrobalan, Chebulic myrobalan, Yellow myrobalan, and Terminalia chebulabe longs to Combretaceae family is a major Ayurvedic medicine that is native to South Asia, predominantly from India. Apart from Ayurveda, it is commonly used in Unani and Homeopathic medicine systems. Because of the broad variety of pharmacological activities connected with the biologically active constitutents found throughout this herb, it is included in conventional medicine. The fruit contains main pharmacological activities such as Hepatoprotective activity, Cytoprotective activity Cardioprotective activity, Antidiabetic and renoprotective activity, Antibacterial activity, Antifungal activity, Antiviral activity, Antiprotozoal activity, Anti-inflammatory and anti-arthritic activity, antioxidant and free radical scavenging activity, Anticarcinogenic activity, Antimutagenic, radioprotective and Chemopreventive activity, Hypolipidemic and hypocholesterolemic activity, Adaptogenic and anti anaphylactic activities, Gastrointestinal motility improving and anti-ulcerogenic activity, Antispasmodic activity, Wound healing activity, Anticaries activity, Immunomodulatory activity any many are reported with scientific evidence. All these ancient applications of Terminalia chebula as home remedies have been confirmed in preclinical trials. The current evidence on the effect of Terminalia chebula intake or consumption on gastrointestinal disorders and diseases is scientifically based on preclinical and clinical trials. All these ancient applications of Terminalia chebula as home remedies have been confirmed in preclinical trials. The current evidence on the effect of Terminalia chebula intake or consumption on gastrointestinal disorders and diseases is scientifically based on preclinical and clinical trials. Study indicates different dosage of Terminalia chebula is effective to get relief from gastrointestinal troubles. Due to less number of gastrointestinal studies, there is no scientific report to consume a specific amount of dose. To prove that Terminalia chebula and its standard extracts are effective as a gastroprotective agent, more comprehensive preclinical and clinical trials are required. To reliably evaluate the appropriate dosages for specific disorders and preparation of extract of Terminalia chebula fruit in prospective human trials procedures, dose-finding preclinical studies should be conducted. There is an evident need for more patient and physician education concerning specific therapies, legislation to regulate the quality of pharmaceutical preparations, and, in particular, more clinical and pre-clinical trials to determine the value and safety of such medicaments in digestive and other disorders.

Anti-Toxoplasma Effect of Hydroalchohlic Extract of Terminalia chebula Retz in Cell Culture and Murine Model

Background: We examined anti-Toxoplasma effect of hydroalcoholic extract of Terminalia chebula Retz (T. chebula) in cell culture and murine model. Methods: The study was conducted in Shahrekord University of Medical Sciences, Iran in 2017. Half maximal effective (concentration (EC50) of T. chebula extract and pyrimethamine was determined in infected Hela cells by using 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) method. In the animal model, BALB/c mice were injected with tachyzoites (104) of T. RH strain intraperitoneally. 24h after the injection, the test groups were orally treated with 100, 200, 400 and 800 mg/kg of T. chebula extract for 7 days. The survival rate of the mice was determined and blood samples were collected to determine the amount of serum Malondialdehyde (MDA) and antioxidant capacity. Then peritoneal fluid of the mice was collected to count the number of tachyzoites and after necropsy, the pathologic changes, including the weight of liver, spleen and kidneys were investigated. The analysis of data was accomplished using SPSS. Results: EC50 values were 94.7μg/mL and 290.50μg/mL for T. chebula and pyrimethamine respectively. In the animal model, the extract of T. chebula in concentration of 100 mg/kg showed the same anti-Toxoplasma effect as pyrimethamine. This concentration of the extract decreased number of intraperitoneal tachyzoites and increased the survival rate of the mice. This extract reduced the levels of serum MDA and tissue inflammation and increased serum antioxidant capacity. Conclusion: Regarding the positive effect of extract, after more clinical trials in the animal model and standardization of the extract, it can be used as an alternative or complementary therapy for toxoplasmosis.

Efficacy of Terminalia chebula) and exercise in management of obesity (Sthaulya) — A case report

Obesity or is one of the lifestyle disorders rapidly increasing in the community; due to rigorously changing dietary habits and behavioural pattern. is a state of increased ofmeta data i.e. abnormal and /or excessive fat accumulation that may impair health of an individual. A married woman aged 29 years, presented to the clinic with complaint of infertility, loss of appetite and increased body weight. Regular walking exercise, as medicine along with counseling for diet restrictions made her lose 7.15% of her body weight with BMI reduction up to 2.94 units. Obesity is a public health problem with multiple risk factors and so its management is also very complex. Essential calorie intake coupled with appropriate adequate physical exercise and proper adherence to Ayurved guidelines for (daily regimen) can reduce rising problem of obesity in society.