High frequency plant regeneration from cotyledonary node explants of Cucumis sativus L. cultivar ‘Green Long’ via adventitious shoot organogenesis and assessment of genetic fidelity by RAPD-PCR technology

Cotyledon explants of four watermelon [Citrullus lanatus (Thunb.) Mataum. & Nakai] breeding lines (F92U8, SP90-1, SP90-2, and SP90-4) were prepared from mature seed or from 2-, 4-, 6-, 8-, or 10-day-old seedlings. Explants were incubated on shoot regeneration medium for 8 weeks followed by 4 weeks on shoot elongation medium. The four genotypes differed in their ability to produce shoots at each explant age. The highest frequency with which F92U8 (66%) and SP90-2 (60%) explants produced shoots was for 2-day-old seedlings. Fewer explants formed shoots when established from mature seed or seedlings older than 2 days. In contrast, the percentage of SP90-4 explants that produced shoots was highest when cotyledons were obtained from 4-day-old seedlings (40%), but the response was less than the optimum for F92U8 and SP90-2. SP90-1 cotyledon explants exhibited the poorest response of the four breeding lines (<11% produced shoots), with little difference in response among the explant ages tested. The number of shoots per responding explant also depended on the age of the explant source. Explants from 2- to 4-day-old seedlings produced the most shoots. Fewer shoots formed on cotyledons from mature seed or seedlings older than 4 days.

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HIGH-FREQUENCY INDUCTION OF MULTIPLE SHOOTS AND PLANT REGENERATION FROM COTYLEDONARY NODE EXPLANTS OF TONGKAT ALI (EURYCOMA LONGIFOLIA JACK)

Applied Ecology and Environmental Research ◽

10.15666/aeer/1805_63216333 ◽

2020 ◽

Vol 18 (5) ◽

pp. 6321-6333

Author(s):

A.G.A. ALTTAHER ◽

Z.N.B. YUSOF ◽

M. MAHMOOD ◽

N.A. SHAHARUDDIN

Keyword(s):

Plant Regeneration ◽

High Frequency ◽

Multiple Shoots ◽

Cotyledonary Node ◽

Eurycoma Longifolia ◽

Eurycoma Longifolia Jack ◽

Cotyledonary Node Explants ◽

High Frequency Induction

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Gelling Agents Affect Adventitious Shoot Organogenesis in White Ash

HortScience ◽

10.21273/hortsci.30.4.873e ◽

1995 ◽

Vol 30 (4) ◽

pp. 873E-873

Author(s):

Sharon A. Bates ◽

John E. Preece ◽

John H. Yopp

Keyword(s):

Liquid Medium ◽

Shoot Growth ◽

Shoot Organogenesis ◽

Adventitious Shoot ◽

Shoot Elongation ◽

Gelling Agent ◽

White Ash ◽

Shoot Initiation ◽

Adventitious Shoot Organogenesis

Dissected white ash seeds were placed on an agar-solidified MS medium with 10 μM TDZ and 1 μM 2,4-D (shoot initiation medium). After 4 weeks, explants were transferred to shoot elongation medium (3 μM TDZ, 1 μM BA, and 1 μM IBA) solidified with 0.7% Sigma agar, 0.525% agar + 0.05% gelrite, 0.35% agar + 0.1% gelrite, 0.175% agar + 0.15% gelrite, 0.2% gelrite, or no gelling agent (liquid medium). After 12 weeks in vitro, shoot growth and number were suppressed in cultures containing 0.2% gelrite (9.3 mm and 0.7 shoots) and in cultures containing no gelling agent (6.9 mm and 0.7 shoots). There were no differences in shoot growth and number in cultures containing 0.7% Sigma agar (2.2 mm and 16.5 shoots), 0.525% agar + 0.05% gelrite (2.6 mm and 18.7 shoots), 0.35% agar + 0.1% gelrite (1.6 mm and 17.4 shoots), and 0.175% agar + 0.15% gelrite (2.0 mm and 20.4 shoots). The most vitrification occurred in cultures on medium with the lowest amount of agar, gelrite only, and liquid medium.

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