Multiple Shoot Induction from Cotyledonary Node Explants of Terminalia chebula

2003 ◽  
Vol 46 (4) ◽  
pp. 585-588 ◽  
Author(s):  
B. Shyamkumar ◽  
C. Anjaneyulu ◽  
C.C. Giri
Keyword(s):  
Cotyledonary Node ◽  
Multiple Shoot ◽  
Terminalia Chebula ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Cotyledonary Node Explants

scholarly journals An efficient shoot regeneration system for medicinally important Elephantopus scaber Linn.

2015 ◽  
Vol 15 (2) ◽  
pp. 94-99 ◽  
Author(s):  
Jyothi Abraham ◽  
T. Dennis Thomas
Keyword(s):  
Age Groups ◽  
Cotyledonary Node ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Shoot Induction ◽  
Regeneration System ◽  
Multiple Shoot Induction ◽  
Elephantopus Scaber ◽  
Cotyledonary Node Explants

An efficient protocol for the rapid micropropagation of medicinally important Elephantopus scaber has been standardized using cotyledonary node explants. Direct multiple shoot induction was observed when the cotyledonary node explants at various age groups were cultured on MS medium supplemented with various plant growth regulators. The highest shoot induction was obtained when the cotyledonary node explants from 20-day-old seedlings were cultured on MS medium supplemented with 1.5 mg L-1 TDZ and 0.5 mg L-1 NAA. On this medium, 98% of the cultures responded, with an average number of 33.7 shoots per explant. The highest frequency of rooting (100%) and mean number of roots (3.3 per shoot) were observed when the shoots were transferred to MS medium supplemented with 1.0 mg L-1 IBA. The plantlets raised in vitro were acclimatized and transferred to soil with a 92% success rate. The protocol described here may be utilized for multiplication and conservation of elite clones of E. scaber.

scholarly journals Rapid in vitro micropropagation of Cicer arietinum L.

2008 ◽  
Vol 34 (No. 1) ◽  
pp. 1-5 ◽  
Author(s):  
G. Sujatha ◽  
N. Jayabalan ◽  
D. Ranjitha Kumari B
Keyword(s):  
Cicer Arietinum ◽  
Multiple Shoots ◽  
Cotyledonary Node ◽  
Multiple Shoot ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Indole Butyric Acid ◽  
Shoot Number

A rapid, simple and efficient protocol for <i>in vitro</i> multiple shoot induction and plantlet regeneration was achieved from three different explants of <i>Cicer arietinum</i>. The explants viz shoot tip, cotyledonary node and node were cultured on MS medium fortified with Benzyl Adenine (BA) (0.44&minus;8.88&mu;M) for multiple shoot induction. Multiple shoots proliferation was best observed at 4.44&mu;M BA from all the three explants within two weeks of culture. Of the three different explants tested, cotyledonary nodes produced the maximum number of shoots. Shoot number per explant ranged between 7 and 15. Individual shoots were aseptically excised and subcultured in the same media for shoot elongation. The elongated shoots were transferred to Indole Butyric Acid (IBA) (2.46&minus;12.30&mu;M) for root induction. Rooting was observed within two weeks of culture. Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions. The recorded survival rate of the plants was 76.3%. Plants looked healthy with no visually detectable phenotypic variations.

Mutagen treatment affects the regeneration efficiency of the shoot-tip explant in Celosia cristate L. (Cockscomb)

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Rupesh S. Badere ◽  
Pallavi K. Rinkey
Keyword(s):  
Plant Growth Regulator ◽  
Gamma Ray ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Induction ◽  
Ethyl Methane Sulphonate ◽  
Multiple Shoot Induction ◽  
Methane Sulphonate ◽  
Regeneration Efficiency

The shoot-tip explant harvested from ethyl methane sulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated seedling induced shoots with 8.8µM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

scholarly journals Direct multiple shoot induction and plant regeneration from dormant buds of Codonopsis pilosula (Franch.) Nannf.

2011 ◽  
Vol 10 (51) ◽  
pp. 10509-10515 ◽  
Author(s):  
H Zhang Y ◽  
F Gao S ◽  
Du T ◽  
G Chen H ◽  
H Z Wang ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Multiple Shoot ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Codonopsis Pilosula
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