Cysticercus bovis in cattle slaughtered in North Egypt: Overestimation by the visual inspection method

Background and Aim: The World Health Organization and the Food and Agriculture Organization list Taenia saginata, a foodborne cestode, as the most widely distributed human tapeworm worldwide. The larval stage of T. saginata, Cysticercus bovis, causes cysticercosis in bovines and infects humans who eat raw or undercooked beef. The existing detection methods of C. bovis in cattle depend on the visual inspection of meat. This study aimed to confirm the identification of C. bovis through visual inspection at the slaughterhouses in North Egypt with a molecular diagnosis. Materials and Methods: A total of 687 locally bred cattle (Baladi), including 428 cows and 259 buffaloes, slaughtered in four slaughterhouses in North Egypt from April 2018 to February 2019 were inspected for C. bovis using the traditional meat inspection method. Positive samples were verified through polymerase chain reaction (PCR) amplification and HDP2 gene sequencing. Results: Through visual inspection, C. bovis was detected in 4.2% and 12.4% of the slaughtered cows and buffaloes, respectively. Molecular analysis confirmed that 1.9% of the animals, all of which were cows, had C. bovis infection. DNA sequencing verified the identity of the PCR-amplified product. Conclusion: The rate of C. bovis infection in slaughterhouses detected through meat inspection is overestimated compared with that through PCR. Although meat inspection can be used as a primary screening tool for C. bovis, a more specific molecular method is required to achieve an accurate diagnosis.

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TRENDS

PEDIATRICS ◽

10.1542/peds.10.5.619 ◽

1952 ◽

Vol 10 (5) ◽

pp. 619-623

Keyword(s):

United Nations ◽

The United States ◽

World Health ◽

United States Government ◽

Food And Agriculture ◽

Equatorial Africa ◽

Food And Agriculture Organization ◽

The United Nations ◽

Health Organization ◽

Emergency Fund

IT APPEARS timely to call attention again to the work and objectives of the United Nations International Children's Emergency Fund. Particularly noteworthy is the trend to use this fund more and more in efforts to help other nations help themselves. Thus the mass attack on tuberculosis, yaws and malaria are, it is hoped, bringing those diseases into proportions where their continued control can be more effectively managed. Similarly, increasing attention is being given to the training of professional and technical personnel. The plans and long-range purpose of the UNICEF have recently been described by Maurice Pate, Executive Director of the fund: "Five years ago, in May 1947, the United Nations International Children's Emergency Fund received its first pledge of support, a contribution of $15,000,000 from the United States Government. A number of other pledges and contributions soon followed, and procurement of supplies was begun. By the middle of 1948, those supplies were reaching several million children. "Those early beginnings were in the minds of many of us at the recent meeting of the Fund's 26-nation Executive Board (April 22-24), for on that occasion UNICEF's aid was extended to the only remaining area of need in which it had not been operating— Africa, south of the Sahara. "In the Belgian Congo, French Equatorial Africa, Liberia, Togoland, the Cameroons and West Africa, UNICEF, side by side with the World Health Organization and the Food and Agriculture Organization, will soon be working with the governments and people on a number of child-health projects. The largest of these is to be an attack on kwashiokor, a dietary deficiency disease that affects thousands of young children in these regions.

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