scholarly journals ISOLATION AND DISTRIBUTION OF CRUDE OIL AND POLYCYCLIC AROMATIC HYDROCARBON-DEGRADING BACTERIA FROM POLLUTED HARBOURS IN NORTH JAKARTA

2015 ◽  
Vol 39 (2) ◽  
pp. 79-85
Author(s):  
Yustian Rovi Alfiansah ◽  
Mindi Adindasari ◽  
Mentari Argarini ◽  
Yeti Darmayati ◽  
Ruyitno
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Crude Oil ◽  
Sea Water ◽  
Enrichment Culture ◽  
Culturable Bacteria ◽  
Water And Sediment ◽  
Diverse Species ◽  
Degrading Bacteria ◽  
Polycyclic Aromatic

Several harbours in North Jakarta have been polluted by spills of oil and their derivates. We suggest that diverse species of crude oil and polycyclic aromatic hydrocarbon-degrading bacteria  inhabit these harbours. An experiment was undertaken in 2007 to isolate crude oil and polycyclic aromatic hydrocarbon (PAH)-degrading bacteria from oil-polluted harbours, such as Muara Baru, Sunda Kelapa and Tanjung Priok. Sea water and sediment samples were collected twice, in March and April. Crude oil and PAH-degrading bacteria were isolated from enrichment culture of samples in an enrichment medium (SWP), using ONR7a medium with the addition of 5 types of PAH gases or Arabian Light Crude Oil 210 (ALCO 210) onto medium. This study reported that fluoranthene and crude oil-degrading bacteria were the major bacteria isolated from the three polluted harbours. In total, 109 isolates have been collected which can degrade crude oil (29% of total isolates), fluoranthene (33%), fluorene (20%), pyrene (7%), dibenzothiopene (6%), and phenantrene (5 %). Among these isolates, 5 isolates have the capability to degrade 5 types of PAH and ALCO 210. They were Alcanivorax sp. B-1084, Pseudomonas sp. D5-38b, Alcanivorax sp. TE-9, Bacillus sp. L41, Alcanivorax dieselolei strain B-5 clone 1. Culturable bacteria have been isolated mostly from the Sunda Kelapa samples, with fewer in those from Muara Baru and Tanjung Priok, respectively

Experimental Study of the Biochemistry Method for Enhancing Oil Recovery in the Oil Reservoir after Polymer Flooding

2013 ◽  
Vol 647 ◽  
pp. 144-149
Author(s):  
Yue Hui She ◽  
Fan Zhang ◽  
Bo Xun Liang ◽  
Zheng Liang Wang ◽  
Long Jiang Yu
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Crude Oil ◽  
Oil Recovery ◽  
Degradation Rate ◽  
Polymer Flooding ◽  
Residual Oil ◽  
Degrading Bacteria ◽  
Profile Control ◽  
Polycyclic Aromatic

A delayed cross linked gel profile control agent is used to plug high permeable formations. Also, well nutrient fluid and microbes are injected with 50% of the heavy residual oil, after polymer flooding, in order to improve oil recovery due to the complex environment of oil reservoirs. Four strains of polycyclic aromatic hydrocarbon-degrading bacteria are selected from oilfield produced water with a high efficiency. Two of the four strains, namely BISYX17 and BISYX14, are new. Polycyclic aromatic hydrocarbon-degrading bacteria have high growth activity and they are able to reach a maximum stain concentration after being cultured 4 to 8 days, using phenanthrene as their sole carbon source. They are able to effectively degrade heavy hydrocarbon with a phenanthrene degradation rate of up to 80％, after the sample is cultured for seven days. Strain BISYX7 has the strongest phenanthrene -degrading ability, with a maximum degradation percentage of 89.89％. The strains are capable of producing dioxygenase to open rings of polycyclic aromatic hydrocarbon. The dioxygenase activity, produced by BISYX17, is able to reach 40.2 IU/mg, which is higher than the enzyme activities of a wild strain. This shows the strain has excellent potential to produce enzymes. Enzymes, produced by metabolism, have a direct degradation rate of 68% on crude oil. A core displacement simulation experiment indicates a profile control oil-displacing system is able to improve crude oil recovery efficiency by 17%, after polymer flooding. Thus, the system has excellent application potential for residual oil recovery.

scholarly journals Polycyclic Aromatic Hydrocarbon Degradation of Phytoplankton-Associated Arenibacter spp. and Description of Arenibacter algicola sp. nov., an Aromatic Hydrocarbon-Degrading Bacterium

2013 ◽  
Vol 80 (2) ◽  
pp. 618-628 ◽  
Author(s):  
Tony Gutierrez ◽  
Glenn Rhodes ◽  
Sara Mishamandani ◽  
David Berry ◽  
William B. Whitman ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Crude Oil ◽  
Marine Diatom ◽  
Content Type ◽  
Degrading Bacterium ◽  
Eukaryotic Phytoplankton ◽  
Degrading Bacteria ◽  
Polycyclic Aromatic ◽  
Taxonomic Marker

ABSTRACTPyrosequencing of the bacterial community associated with a cosmopolitan marine diatom during enrichment with crude oil revealed severalArenibacterphylotypes, of which one (OTU-202) had become significantly enriched by the oil. Since members of the genusArenibacterhave not been previously shown to degrade hydrocarbons, we attempted to isolate a representative strain of this genus in order to directly investigate its hydrocarbon-degrading potential. Based on 16S rRNA sequencing, one isolate (designated strain TG409T) exhibited >99% sequence identity to three type strains of this genus. On the basis of phenotypic and genotypic characteristics, strain TG409Trepresents a novel species in the genusArenibacter, for which the nameArenibacter algicolasp. nov. is proposed. We reveal for the first time that polycyclic aromatic hydrocarbon (PAH) degradation is a shared phenotype among members of this genus, indicating that it could be used as a taxonomic marker for this genus. Kinetic data for PAH mineralization rates showed that naphthalene was preferred to phenanthrene, and its mineralization was significantly enhanced in the presence of glass wool (a surrogate for diatom cell surfaces). During enrichment on hydrocarbons, strain TG409Temulsifiedn-tetradecane and crude oil, and cells were found to be preferentially attached to oil droplets, indicating an ability by the strain to express cell surface amphiphilic substances (biosurfactants or bioemulsifiers) as a possible strategy to increase the bioavailability of hydrocarbons. This work adds to our growing knowledge on the diversity of bacterial genera in the ocean contributing to the degradation of oil contaminants and of hydrocarbon-degrading bacteria found living in association with marine eukaryotic phytoplankton.

scholarly journals Isolasi Bakteri Pendegradasi Minyak Mentah dan Polisiklik Aromatik Hidrokarbon dari Sedimen Mangrove Bintan

2019 ◽  
Vol 4 (3) ◽  
pp. 155
Author(s):  
Nur Fitriah Afianti ◽  
Deva Febrian ◽  
Dede Falahudin
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Crude Oil ◽  
Oil Spills ◽  
Rrna Genes ◽  
Mangrove Sediments ◽  
Indigenous Bacteria ◽  
Sediment Samples ◽  
Degrading Bacteria ◽  
Polycyclic Aromatic

<strong>Isolation of Crude Oil and Polycyclic Aromatic Hydrocarbon-Degrading Bacteria from Mangrove Sediments in Bintan.</strong> Bintan is known for its extensive mangrove areas, but it is prone to pollution from oil spills due to it is close to the international shipping lane. Indigenous bacteria plays important roles in bioremediation of oil spills in the natural environment. This research aims to explore indigenous bacteria from Bintan’s mangrove sediments which may have ability to degrade crude oil and polycyclic aromatic hydrocarbon (PAH). The mangrove sediment samples were taken in March 2018 from the sediments near four different mangrove plant species, i.e. <em>Rhizophora apiculata, Xylocarpus granatum, Ceriops tagal </em>and <em>Lumnitzera littorea</em>. Isolation of oil degrading bacteria was carried out using enrichment media supplemented with crude oil ALCO. A total of 45 strains of oil degrading bacteria were successfully isolated from the sediment samples. By using sublimation method, 13 bacterial isolates showed the ability to degrade various PAHs, including phenanthrene, acenaphthene, dibenzothiophene and fluorene. Sequencing analysis of 16s rRNA genes confirmed that the 13 isolated bacteria belong to the genera <em>Rhodococcus, Bacillus</em>, <em>Sphingopyxis</em>, <em>Rhizobium</em>, <em>Mycobacterium</em>, and <em>Gordonia</em>

scholarly journals Heterologous Expression of Polycyclic Aromatic Hydrocarbon Ring-Hydroxylating Dioxygenase Genes from a Novel Pyrene-Degrading Betaproteobacterium

2012 ◽  
Vol 78 (10) ◽  
pp. 3552-3559 ◽  
Author(s):  
David R. Singleton ◽  
Jing Hu ◽  
Michael D. Aitken
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Enrichment Culture ◽  
Metagenomic Library ◽  
Stable Isotope Probing ◽  
Content Type ◽  
Close Proximity ◽  
Polycyclic Aromatic ◽  
Dominant Member ◽  
Pah Metabolism

ABSTRACTA betaproteobacterium within the familyRhodocyclaceaepreviously identified as a pyrene degrader via stable-isotope probing (SIP) of contaminated soil (designated pyrene group 1 or PG1) was cultivated as the dominant member of a mixed bacterial culture. A metagenomic library was constructed, and the largest contigs were analyzed for genes associated with polycyclic aromatic hydrocarbon (PAH) metabolism. Eight pairs of genes with similarity to the α- and β-subunits of ring-hydroxylating dioxygenases (RHDs) associated with aerobic bacterial PAH degradation were identified and linked to PG1 through PCR analyses of a simplified enrichment culture. In tandem with a ferredoxin and reductase found in close proximity to one pair of RHD genes, six of the RHDs were cloned and expressed inEscherichia coli. Each cloned RHD was tested for activity against nine PAHs ranging in size from two to five rings. Despite differences in their predicted protein sequences, each of the six RHDs was capable of transforming phenanthrene and pyrene. Three RHDs could additionally transform naphthalene and fluorene, and these genotypes were also associated with the ability of theE. coliconstructs to convert indole to indigo. Only one of the six cloned RHDs was capable of transforming anthracene and benz[a]anthracene. None of the tested RHDs were capable of significantly transforming fluoranthene, chrysene, or benzo[a]pyrene.

Sign in / Sign up

Export Citation Format

Share Document