Metagenomic Analysis of Microbial Communities From Acidic Marshland Habitat of India

Understanding the diversity and functioning of microbial communities in acidic marsh land is extremely less investigated in contrast to soils and aquatic ecosystems. This study implemented Illumina high-throughput sequencing to explore the microbial communities and xenobiotic degrading enzymes in the acidic marshland. Taxonomic analysis using SILVA SSU database stated that Proteobacteria (66.74 %), Bacteroidetes (6.98%) and acidobacteria (2.35 %) were the most abundant phylum in the acidic marshland. Functional analysis by SEED subsystems showed that 1.62 % substitute to metabolism of aromatic compound while KO indicates 1.86% of metabolism are involved in xenobiotic biodegradation. KO analysis also indicated the benzoate degradation pathway (ko00362) are predominant while Chlorocyclohexane and chlorobenzene degradation pathway (ko00361), Polycyclic aromatic hydrocarbon degradation pathway (ko00624) Aminobenzoate degradation pathway (ko00627) is largely present in the acidic marshland.

Synthesis of Biochar-Supported K-doped g-C3N4 Photocatalyst for Enhancing the Polycyclic Aromatic Hydrocarbon Degradation Activity

The development of novel and green photocatalysts have attracted considerable attentions due to their excellent performance for environmental remediation, especially for the degradation of persistent pollutants. In this work, the biochar-supported K-doped g-C3N4 composites with the high photocatalytic activity under visible light irradiation was prepared by the calcination-impregnation method. The crystal structure, apparent morphology and functional group composition of the as-prepared photocatalytic materials were studied by X-ray diffraction (XRD), scanning electron microscopy (SEM) and Fourier transform infrared spectroscope (FTIR). Moreover, the characterization of UV-Vis diffuse reflectance spectra (UV-Vis DRS) and photoluminescence technique (PL) verified the good optical properties of resultant samples. Naphthalene was selected as the representative compound to evaluate the photocatalytic performance of the prepared photocatalysts under visible light irradiation. The evaluation results showed that the biochar-supported K-doped g-C3N4 composites exhibited excellent photocatalytic activity (82.19%). Moreover, the photocatalytic degradation rate basically remained unchanged after five cycles, indicating the good stability of the prepared photocatalysts. In addition, a possible mechanism for the photodegradation process was proposed on the basis of the main intermediates detected by gas chromatography-mass spectrometer (GC-MS). This study may provide a promising approach for the polycyclic aromatic hydrocarbon degradation by waste utilization of agricultural biomass and increasing the photocatalytic performance of pure g-C3N4.