Comparative Studies on Isolation and Characterization of Allinase from Garlic and Onion using PEGylation-A Novel Method

Ferrography is a method for separating wear particles onto a slide. The method is based on the interaction between an external magnetic field and the magnetic moments of the particles suspended in a flow stream. It is advantageous in providing high detectability rate for a relatively large range of particle sizes (0.5–200 μm) [1]. A newer generation of ferrography, known as Bio-Ferrography, allows particles from five fluid samples to be isolated simultaneously on one slide and analyzed in terms of their number, chemistry, shape, dimensions, surface morphology, structure, etc. Since magnetization does not naturally occur in polymeric and biological materials, wear particles of such origins must be magnetized prior to Bio-Ferrography. This can be done, for instance, by binding to a ferromagnetic element, such as Er+3, originating from erbium chloride (ErCl3) solution. Such Bio-Ferrography technology has already been applied successfully in hip wear simulations for the separation of ultrahigh molecular weight polyethylene (UHWPE) wear debris suspended in bovine serum as lubricant [2].

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Isolation and characterization of phenotypes of mycobacteria

Canadian Journal of Microbiology ◽

10.1139/m76-131 ◽

1976 ◽

Vol 22 (7) ◽

pp. 908-914 ◽

Cited By ~ 1

Author(s):

Raymond Turcotte ◽

Marc Quevillon

Keyword(s):

Comparative Studies ◽

Parental Strain ◽

Pure State ◽

Antigenic Composition ◽

Cultural Conditions ◽

Isolation And Characterization

Several strains of mycobacteria grown as surface pellicle on liquid Sauton's medium under semianaerobic conditions dissociated into three phenotypes: phenotypes 1, 2, and 3. Only phenotype 1 could be obtained in a pure state. None of these phenotypes was found to be stable: they convert from one into another and all revert to the parental strain when replaced in their usual aerobic cultural conditions. Comparative studies of phenotypes 1 and 3 have shown that significant differences exist in their physiological behaviour and antigenic composition.

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Isolation and Characterization of Nocardiae Associated with Foaming Coastal Marine Waters

Pathogens ◽

10.3390/pathogens10050579 ◽

2021 ◽

Vol 10 (5) ◽

pp. 579

Author(s):

Luke Wright ◽

Mohammad Katouli ◽

D. Ipek Kurtböke

Keyword(s):

Direct Contact ◽

Marine Environments ◽

Marine Animals ◽

Preventative Measures ◽

Isolation And Characterization ◽

Coastal Marine ◽

Near Shore ◽

Molecular Identification Methods ◽

Novel Method

Nocardiosis is an infectious disease caused by Nocardia species that occurs worldwide, albeit more prevalently in tropical/subtropical regions. It can appear as either acute, subacute or as a chronic infection mostly with those with a compromised/weakened immune system. Inhalation of spores and or mycelium fragments is the main transmission route for developing pulmonary nocardiosis. In contrast, cutaneous nocardiosis usually occurs via direct contact. In the subtropical region of the Sunshine Coast in Australia foaming events with thick and persistent and orange-brown color foam have been observed during summer seasons in the near shore marine environments. This study reports the existence of nocardiae in these near shore marine environments by the use of a novel isolation method which used the gas requirements of nocardiae as a selective battery. A total of 32 nocardiae were isolated with the use of this novel method and subsequently conducted molecular identification methods confirmed that the isolates belonged to the genus Nocardia. Twenty-one isolates out of the 32 were closely related to N. nova strains MGA115 and one was related to CBU 09/875, in addition when compared with human pathogenic nocardiae twenty of the isolates were found to be related to N. nova strain JCM 6044. Isolates displayed varied resistance against some of the antibiotics tested when interpretation threshold recommended the Comite de L’Antibiogramme de la Societe Francaise de Microbiologie were used. The highest level of resistance against cefotaxime (n = 27) and ceftriaxone (n = 24). Some of the isolates (n = 6) that displayed resistance to selected antibiotics also possessed potential human pathogenic characteristics such as adherence and translocation through human long epithelial cells as well as displaying phage resistance (n = 26). They might thus present a potential public health risk if frequently encountered through exposure to aerosols generated by the foam as well as direct contact through a wound. Preventative measures to control the growth of nocardiae in such environments such as the control of pollutants, might prevent potential infections that might be caused by these bacteria in humans as well as in marine animals.

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