scholarly journals Utility of Rapid Diagnostic Tests for Detection of Malarial Antigens and Their Comparison with Peripheral Blood Smear Examination

2020 ◽  
Vol 9 (15) ◽  
pp. 1291-1294
Author(s):  
Shreshy Singh ◽  
Sangeeta Dey Akoljam ◽  
Dhananjay Kumar ◽  
Aninda Sen ◽  
Ashit Kumar
Keyword(s):  
Peripheral Blood ◽  
Diagnostic Tests ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Rapid Diagnostic Tests ◽  
Smear Examination ◽  
Blood Smear Examination

scholarly journals Erythrocyte Morphology in Women of Reproductive Age (WORA) with Anemia

Biomedika ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 83-91
Author(s):  
Alex Jekson Tukira ◽  
Lucia Sincu Gunawan ◽  
Edy Prasetya
Keyword(s):  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Reproductive Age ◽  
Target Cells ◽  
Women Of Reproductive Age ◽  
Erythrocyte Morphology ◽  
Smear Examination ◽  
Blood Smear Examination ◽  
Laboratory Technology

Anemia is a decrease in the number of erythrocytes in the blood circulation or the level of hemoglobin that is less than normal. The three major body mechanisms that cause anemia are excessive destruction of erythrocytes, blood loss, and decreased erythrocyte production. Based on the Basic Health Research (Riskesdas) in 2013, the prevalence of anemic women of reproductive age (WORA) aged 15-44 years in Indonesia was 35.3%. Anemia is classified based on the morphology of erythrocytes, including hypochromic microcytic, normocytic normochromic, and macrocytic. Erythrocyte morphology can be observed using peripheral blood smear examination. The objective of this study was to determine the morphology of erythrocytes in anemic women of reproductive age. This study belongs to descriptive research. The population of the study was 136 women of reproductive age, covering the students of D-IV in Medical Laboratory Technology at Setia Budi University. Forty-one respondents suffering from anemia were taken using a purposive sampling technique. The types of anemia were determined with examination using an Easy Touch hemoglobinometer with the Hb level of less than 12g/dL. Preparation of peripheral blood smear examination using EDTA venous blood and stained with Giemsa. Microscopic examination was performed with 1000x objective magnification. The peripheral blood smear reading showed the erythrocyte morphology that includes normocytic normochromic (38 samples or 93%), microcytic hypochromic (three samples or 7%), and poikilocytosis consisting of teardrop cells, target cells, ellipstocytes, and stomatocytes (five samples or 18%). Further study is required to investigate the correlation of erythrocyte index and peripheral blood smear in anemia.

Peripheral Blood Smear Examination

2012 ◽  
pp. 371-371
Author(s):  
Ramadas Nayak ◽  
Sharada Rai ◽  
Astha Gupta
Keyword(s):  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Smear Examination ◽  
Blood Smear Examination

scholarly journals A Comparative Study of Diagnostic Methods of Malaria - Microscopy Versus Rapid Diagnostic Test Kits in a Tertiary Care Hospital, Rajasthan

2021 ◽  
Vol 8 (22) ◽  
pp. 1826-1830
Author(s):  
Saumya Singh ◽  
Neelam Chauhan ◽  
Jyoti Tomar ◽  
Aditya Pratap Singh
Keyword(s):  
Diagnostic Test ◽  
Peripheral Blood ◽  
Diagnostic Tests ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Rapid Diagnostic Tests ◽  
Diagnostic Methods ◽  
Test Method ◽  
Malarial Parasite ◽  
Card Test

BACKGROUND Malaria is one of the most prevalent parasitic diseases in the world including India. Majority of malarial cases are found in countries where cost-effectiveness, diagnostic test performance ease, and trained personnel, are the most important considerations. We wanted to compare the two methods of rapid diagnostic tests (RDTs) and microscopy in the diagnosis of malaria parasite infection. METHODS We studied 2,723 blood samples of patients who presented with signs and symptoms of malaria from out patient department (OPD) and various wards of Pacific Institute of Medical Science, Udaipur, Rajasthan, from Oct 2018 to Oct 2019. All samples obtained were first tested by RDTs and then the same samples were used to make peripheral blood film (PBF) for microscopy. RESULTS During the 1-year period, rapid card test method showed 178 positives for malarial parasite. Of these, 64 cases were positive for P. vivax and 108 cases for P. falciparum. Peripheral blood smear method showed 160 cases positive for malarial parasite. Of these, 55 cases were positive for P. vivax and 99 cases for P. falciparum and 6 cases of co-infection. PBF failed to detect 9 cases of P. vivax and 9 cases of P. falciparum which were positive by rapid card test. So, 18 cases reported PBF negative. Among these patients, there were 83 females and 95 males. Most affected age group was 16 – 30 years followed by 31 – 45 years. Maximum samples were from the month of October 19 with a positivity rate 35.4 %. CONCLUSIONS RDTs based on malaria antigen (whole blood) method is as specific and more sensitive than microscopy (which is being considered as the gold standard method). Peripheral blood smear method still remains superior for accurate species differentiation, quantitation of parasite and maintaining a permanent record. KEYWORDS Microscopy, Malaria, Rapid Diagnostic Tests

scholarly journals Comparison of peripheral blood smear examination with automated haematology analyzer for diagnosing different types of anemia.

2021 ◽  
Vol 28 (10) ◽  
pp. 1433-1437
Author(s):  
Rabiah Asghar ◽  
Javera Tariq ◽  
Nabeela Naeem ◽  
Anila Zafar ◽  
Khadija Qureshi ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Hypochromic Anemia ◽  
Peripheral Blood Smear ◽  
Smear Examination ◽  
Blood Smear Examination ◽  
Hematology Analyzer ◽  
Different Types ◽  
Automated Hematology Analyzer

Objective: This study aims to determine diagnostic accuracy of peripheral blood smear and automated haematology analyzer and to determine frequency of different types of anemia diagnosed by peripheral blood smear and automated hematology analyzer. Study Design: Cross Sectional study. Setting: Department of Pathology, Rawal Institute of Health Sciences, Islamabad. Period: November 2015 to April 2016. Material & Methods: Sample size of 149 suspected anemia patients was calculated using WHO calculator with 95% confidence interval. Research approval was taken from hospital ethical board. Patients were approached through non probability consecutive sampling method. Both peripheral blood smear examination and automated haematology analysis of each sample was performed. Diagnostic accuracy and frequency of anemia types was measured. Data analysis was done with the help of SPSS version 25. Chi-square and fissure exact test and ROC curve analysis was applied and significant (p<0.05) results were reported. Results: Total 149 patients were included in study. There were 42(28.2%) male and 107(71.8%) female.  Mean age of patients was 35.1±2.1SD. Peripheral blood smear and automated haematology analyzer showed sensitivity (68% vs 92%), specificity (59% vs 88%), PPV (72% vs 92%), NPV (55% vs 88%) and diagnostic accuracy (64% vs 91) respectively. Most common type of anemia diagnosed with peripheral blood smear was microcytic hypochromic anemia with raised RDW 36.7% followed by normocytic normochromic anemia with raised RDW 13.3% and macrocytic anemia (p=0.001) while in automated haematology analyzer microc ytic hypochromic anemia with raised RDW54.4% followed by normochromic normocytic anemoia with normal RDW 11.1% (p=0.000). Conclusion: Automated haematyology analyzer had high diagnostic accuracy for diagnosis of anemia.  Microcytic hypochromic anemia and normocytic normochromic are most common anemias diagnosed by peripheral blood smear and automated hematology analyzer and peripheral blood smear cannot be completely replaced by automated haemolytic analyzer. However, if both methods are used simultaneously, more accurate results can be obtained.

Sign in / Sign up

Export Citation Format

Share Document