High throughput screening of stem cell-material surface interactions used in regenerative reconstruction of large bicortical defects in a minipig model

2021 ◽  
Author(s):  
◽  
Baboucarr Lowe
Keyword(s):  
Stem Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Surface Interactions ◽  
Material Surface ◽  
Cell Material

scholarly journals A Novel High-Throughput Screening Platform Identifies Itaconate Derivatives from Marine Penicillium antarcticum as Inhibitors of Mesenchymal Stem Cell Differentiation

Marine Drugs ◽  
2020 ◽  
Vol 18 (4) ◽  
pp. 192
Author(s):  
Pietro Marchese ◽  
Nipun Mahajan ◽  
Enda O’Connell ◽  
Howard Fearnhead ◽  
Maria Tuohy ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Small Molecules ◽  
High Throughput ◽  
High Throughput Screening ◽  
Chondrogenic Differentiation ◽  
Stem Cell Differentiation ◽  
Ectopic Calcification ◽  
Tissue Calcification ◽  
Screening Platform

Worldwide diffused diseases such as osteoarthritis, atherosclerosis or chronic kidney disease are associated with a tissue calcification process which may involve unexpected local stem cell differentiation. Current pharmacological treatments for such musculoskeletal conditions are weakly effective, sometimes extremely expensive and often absent. The potential to develop new therapies is represented by the discovery of small molecules modulating resident progenitor cell differentiation to prevent aberrant tissue calcification. The marine environment is a rich reserve of compounds with pharmaceutical potential and many novel molecules are isolated from macro and microorganisms annually. The potential of small molecules synthetized by marine filamentous fungi to influence the osteogenic and chondrogenic differentiation of human mesenchymal stem/stromal cells (hMSCs) was investigated using a novel, high-throughput automated screening platform. Metabolites synthetized by the marine-derived fungus Penicillium antarcticum were evaluated on the platform. Itaconic acid derivatives were identified as inhibitors of calcium elaboration into the matrix of osteogenically differentiated hMSCs and also inhibited hMSC chondrogenic differentiation, highlighting their capacity to impair ectopic calcification. Bioactive small molecule discovery is critical to address ectopic tissue calcification and the use of biologically relevant assays to identify naturally occurring metabolites from marine sources represents a strategy that can contribute to this effort.

High-throughput screening of human induced pluripotent stem cell-derived brain organoids

2020 ◽  
Vol 335 ◽  
pp. 108627 ◽  
Author(s):  
Madel Durens ◽  
Jonathan Nestor ◽  
Madeline Williams ◽  
Kevin Herold ◽  
Robert F. Niescier ◽  
...  
Keyword(s):  
Stem Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Induced Pluripotent

scholarly journals High-throughput Screening in Embryonic Stem Cell-derived Neurons Identifies Potentiators of α-Amino-3-hydroxyl-5-methyl-4-isoxazolepropionate-type Glutamate Receptors

2010 ◽  
Vol 285 (22) ◽  
pp. 17209-17217 ◽  
Author(s):  
John McNeish ◽  
Marsha Roach ◽  
John Hambor ◽  
Robert J. Mather ◽  
Laura Weibley ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Glutamate Receptors ◽  
High Throughput ◽  
High Throughput Screening ◽  
Embryonic Stem

scholarly journals Combinatorial Biomolecular Nanopatterning for High-Throughput Screening of Stem-Cell Behavior

2015 ◽  
Vol 28 (7) ◽  
pp. 1472-1476 ◽  
Author(s):  
Yacoub Y. I. Amin ◽  
Kasper Runager ◽  
Fabio Simoes ◽  
Adam Celiz ◽  
Vincenzo Taresco ◽  
...  
Keyword(s):  
Stem Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cell Behavior

High-Throughput Screening for Modulators of Mesenchymal Stem Cell Chondrogenesis

2008 ◽  
Vol 36 (11) ◽  
pp. 1909-1921 ◽  
Author(s):  
Alice H. Huang ◽  
Nuzhat A. Motlekar ◽  
Ashley Stein ◽  
Scott L. Diamond ◽  
Eileen M. Shore ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
High Throughput ◽  
High Throughput Screening

scholarly journals Multiplexing Spheroid Volume, Resazurin and Acid Phosphatase Viability Assays for High-Throughput Screening of Tumour Spheroids and Stem Cell Neurospheres

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e103817 ◽  
Author(s):  
Delyan P. Ivanov ◽  
Terry L. Parker ◽  
David A. Walker ◽  
Cameron Alexander ◽  
Marianne B. Ashford ◽  
...  
Keyword(s):  
Stem Cell ◽  
Acid Phosphatase ◽  
High Throughput ◽  
High Throughput Screening ◽  
Tumour Spheroids

scholarly journals The development of high-throughput screening approaches for stem cell engineering

2007 ◽  
Vol 11 (4) ◽  
pp. 388-393 ◽  
Author(s):  
Ying Mei ◽  
Michael Goldberg ◽  
Daniel Anderson
Keyword(s):  
Stem Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cell Engineering ◽  
Stem Cell Engineering ◽  
Screening Approaches

scholarly journals High-throughput screening of stem cell therapy for globoid cell leukodystrophy using automated neurophenotyping of twitcher mice

2013 ◽  
Vol 236 ◽  
pp. 35-47 ◽  
Author(s):  
Brittni A. Scruggs ◽  
Annie C. Bowles ◽  
Xiujuan Zhang ◽  
Julie A. Semon ◽  
Evan J. Kyzar ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Therapy ◽  
High Throughput ◽  
Stem Cell Therapy ◽  
High Throughput Screening ◽  
Globoid Cell Leukodystrophy ◽  
Globoid Cell
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