scholarly journals Morpho-Anatomical Analysis of Cosmostigma racemosum (Asclepiadoideae) Flowers

2014 ◽  
Vol 3 (1) ◽  
pp. 35
Author(s):  
Widodo Widodo ◽  
Mohamad Amin ◽  
Mimien Henie Irawati Al-Muhdar ◽  
Muhammad Ja’far Luthfi

<em>Cosmostigma racemosum</em> is a plant species belonging to the family Apocynaceae, the subfamily Asclepiadoideae. <em>Cosmostigma racemosum </em>is found in Nglanggeran Mountain Gunungkidul, Yogyakarta. The local name and its original distribution are not known. Information or study of <em>Cosmostigma racemosum</em> in Indonesia is not available. Comprehensive characterization of this species is important for authentication and addition of data base. Characterization was conducted by analyzing the morphology and anatomy of flower. The objectives of this study were to describe and analyze the morphology and anatomy of <em>C. racemosum</em> flowers.  The method of research was based on observation method and exploration of plant systematics evidence or taxonomy evidence, including analyses and description of morphology and anatomy of flower structure and its development.  The results showed that the characteristics of flower morphology are in accordance with the existing description in literatures. Characteristics of pollinia are specific characters of morphological aspect of flower. Data of anatomy of flower and its parts development are the new ones which confirm the position of <em>C.racemosum</em> as a member of the tribe Marsdenieae. The data of anatomy also show new information of the ontogeny of the important parts of flower: pollinia formation, pollinia corpusculum, anther wall, anther sac, stigma, stamen, staminal tube, stigmatic chamber, and structure of ovary in Asclepiadoideae

1979 ◽  
Author(s):  
M Ribieto ◽  
J Elion ◽  
D Labie ◽  
F Josso

For the purification of the abnormal prothrombin (Pt Metz), advantage has been taken of the existence in the family of three siblings who, being double heterozygotes for Pt Metz and a hypoprothrombinemia, have no normal Pt. Purification procedures included barium citrate adsorption and chromatography on DEAE Sephadex as for normal Pt. As opposed to some other variants (Pt Barcelona and Madrid), Pt Metz elutes as a single symetrical peak. By SDS polyacrylamide gel electrophoresis, this material is homogeneous and appears to have the same molecular weight as normal Pt. Comigration of normal and abnormal Pt in the absence of SDS, shows a double band suggesting an abnormal charge for the variant. Pt Metz exhibits an identity reaction with the control by double immunodiffusion. Upon activation by factor Xa, Pt Metz can generate amydolytic activity on Bz-Phe-Val-Arg-pNa (S2160), but only a very low clotting activity. Clear abnormalities are observed in the cleavage pattern of Pt Metz when monitored by SDS gel electrophoresis. The main feature are the accumulation of prethrombin l (Pl) and the appearance of abnormal intermediates migrating faster than Pl.


2014 ◽  
Vol 137 (1) ◽  
pp. 86-95 ◽  
Author(s):  
Juan Cui ◽  
Yanbin Yin ◽  
Qin Ma ◽  
Guoqing Wang ◽  
Victor Olman ◽  
...  

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