Determination of basophil activation and IgE antibodies to the components of dental materials in cases of intolerance to dental prosthetics

The aim of the study was to determine the activation of blood basophils and IgE antibodies to the components of dental materials (CDM) in patients with intolerance to dental prosthetics. Materials and methods: we examined 22 patients with positive skin application tests (AT) with metal salts solutions (NiCl2 (3%), CrCl3 (3%), CoCl2 (1%) and complaints of intolerance to dental materials (IDM), and 21 patients without IDM complaints. We determined the levels of CD203c+CD63+ basophils after incubation of blood with incubation liquid containing particles of prosthetics (LPP) and metal salts solutions (MSS): NiCl2, CrCl3, CoCl2, ZnCl2, MnCl2, also measured IgE antibodies to these salts using ELISA. Results: in patients with IDM and skin hypersensitivity to metal salts an increase or decrease of CD203c+basophils after incubation of basophils with LPP and MSS occurred. In patients with skin hypersensitivity to CDM basophils significantly more often and stronger reacted with LPP and metal salts compared to basophils of control group patients. It was established that LPP had the highest diagnostic efficiency (sensitivity 72,7%; specificity 90,5%; AUC 0,86; p=0,0003; with threshold of expression growth for CD203c+63+ basophils equaling 16,68%), compared to MSS as basophil activation inductor. Conclusion: basophil activation test and IgE antibodies determination are recommended for detection of hypersensitivity and allergy in patients with IDM.

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Characteristics of the eosinophilic fenotype of bronchial asthma in children

Immunopathology Allergology Infectology ◽

10.14427/jipai.2017.1.52 ◽

2017 ◽

pp. 52-57

Author(s):

E Asiryan ◽

N Titova

Keyword(s):

Bronchial Asthma ◽

Skin Tests ◽

Control Group ◽

Ige Receptor ◽

Positive Skin ◽

Ige Antibodies ◽

Total Ige ◽

Atopic Bronchial Asthma ◽

Ige Receptors ◽

High Level

The purpose of the work is clinical and laboratory examination of children with an eosinophilic phenotype of bronchial asthma. Materials and methods. 160 children aged 5 to 18 years were examined: 130 patients with atopic bronchial asthma, 30 children of the control group. During the work, the level of eosinophils, the level of eosinophils carrying FcεRI+ and CD23+IgE+ receptor, the level of total IgE, IgE antibodies to domestic and epidermal allergens in the blood serum were determined, clinical and anamnestic data were studied. Results. In children with atopic bronchial asthma, the relative and absolute content of eosinophils, as well as eosinophils carrying FcεRI+ and CD23+IgE+ receptors, is statistically significantly higher than in the control group (p<0.001). In 71 (54.62%) children with bronchial asthma, eosinophilia was detected, with 58 (81.69%) patients having a high level of eosinophils bearing the FcεRI + receptor and 56 (78.87%) children with a high level of eosinophils carrying CD23+IgE+ receptor. In the group of children with bronchial asthma, a correlation was established between the level of total IgE and the level of eosinophils carrying FcεRI+ (r=+0.389; p<0.01) and CD23+IgE+ receptors (r=+ 0.677; p<0.0001). In children with eosinophilia, in most cases, the hereditary nature of the disease is observed, the first manifestations of allergy are already present in the first year of life, concomitant allergopathology has been established. The optimal effect in the treatment of patients with eosinophilia was obtained with the appointment, as a basic therapy, of inhaled glucocorticosteroids, as well as short-acting β2-agonists for arresting episodes of bronchial obstruction. In most patients with asthma who have eosinophilia, serum levels of IgE antibodies to Dermatophagoides pteronyssinus, to the coat of the cat and the dog's coat are revealed, and positive skin scarification tests with these allergens are detected. Conclusion. In children with atopic bronchial asthma, there are signs of an eosinophilic phenotype: eosinophilia, high expression of FcεRI+ and CD23+IgE+ receptors, increased levels of total IgE and IgE antibodies, positive skin tests with domestic and epidermal allergens, hereditary predisposition, effectiveness of inhaled glucocorticosteroids.

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IgE and IgG4 Repertoire in Asymptomatic HDM-Sensitized and HDM-Induced Allergic Rhinitis Patients

International Archives of Allergy and Immunology ◽

10.1159/000517824 ◽

2021 ◽

pp. 1-12

Author(s):

Qingxiu Xu ◽

Qing Jiang ◽

Lin Yang ◽

Wenjing Li ◽

Nan Huang ◽

...

Keyword(s):

Allergic Rhinitis ◽

Specific Ige ◽

Basophil Activation ◽

Control Group ◽

Positive Skin Prick Test ◽

Positive Skin ◽

Allergic Symptoms ◽

Der P2 ◽

Der P1 ◽

Asymptomatic Subjects

Introduction: Asymptomatic sensitization is defined as the presence of positive skin prick test (SPT) and/or positive serum allergen-specific IgE in the absence of clinical allergic symptoms. Currently, there is no convincing explanation why some people with positive allergen tests do not show symptoms. We aimed to investigate the house dust mite (HDM)-specific IgE and IgG4 repertoire in asymptomatic HDM-sensitized subjects and HDM-induced allergic rhinitis (AR) patients. Methods: A total of 48 subjects sensitized to HDM were included in this study: 27 had AR with/without asthma (symptomatic group), and 21 had no allergic symptoms (asymptomatic group). Six healthy individuals served as control group. Peripheral blood samples were collected for serum IgE and IgG4 assay and basophil activation tests (BATs). IgE and IgG4 assay included antibodies to Dermatophagoides (Der) p1, 2, 7, 10, 21, 23, and Der f1, 2. Results: AR patients had a larger wheal diameter of SPT (7.0 vs. 3.0 mm, p < 0.0001) and a higher specific IgE to Der p (15.50 vs. 0.70 KU/L, p < 0.0001) than asymptomatic subjects. They also showed more frequent sensitization to Der p1 and Der p2 (both p < 0.05). However, the total IgE and specific IgG4 did not differ significantly between the 2 groups. The basophil activation response after being stimulated with HDM was observed to be higher in AR patients (all p < 0.05). Conclusions: There are differences in SPT, serum-specific IgE to Der p, component allergen Der p1 and Der p2 level and BAT between AR patients and asymptomatic subjects sensitized to HDM. IgG4 alone cannot differentiate asymptomatic individuals from AR patients.

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Influence of Antihistamines on Basophil Activation Test in Food Allergy to Milk and Egg

Diagnostics ◽

10.3390/diagnostics11010044 ◽

2020 ◽

Vol 11 (1) ◽

pp. 44

Author(s):

Eleonora Nucera ◽

Riccardo Inchingolo ◽

Rosario Nicotra ◽

Manuela Ferraironi ◽

Anna Giulia Ricci ◽

...

Keyword(s):

Food Allergy ◽

Food Challenge ◽

Basophil Activation Test ◽

Basophil Activation ◽

Double Blind ◽

Positive Skin ◽

Activation Test ◽

History Of ◽

Ige Mediated ◽

Food Allergy Diagnosis

Background: The basophil activation test (BAT) is used to improve the accuracy of food allergy diagnosis. To date, the influence of antiallergic drugs on BAT reactivity is poorly investigated. The aim of the study was to investigate if BAT results were influenced by antihistamine intake for 3 months in a cohort of patients with IgE-mediated food allergy to milk or egg. Methods: A retrospective, single-center, observational study was performed. We enrolled subjects with history of hypersensitivity reaction after specific food ingestion, positive skin prick tests and specific IgEs, concomitant allergic rhinitis, and, contraindication to the double-blind, placebo-controlled food challenge due to personal history of systemic reactions related to the ingestion of culprit food. Validated allergens (α-lactoalbumin, β-lactoglobulin, casein, egg white, and yolk) for BAT were used. Results: Thirty-nine patients with well-documented food symptoms and positive allergological workup were included in the study. BAT was positive in 29 patients. The mean percentages of CD63+ expression to specific culprit allergen did not change after the administration of drugs. Conclusions: This was the first study assessing the effects of oral antihistamines on basophil reactivity in cow’s milk and egg food allergy. Antihistamines do not interfere with BAT results.

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