scholarly journals Characteristics of the eosinophilic fenotype of bronchial asthma in children

2017 ◽  
pp. 52-57
Author(s):  
E Asiryan ◽  
N Titova
Keyword(s):  
Bronchial Asthma ◽  
Skin Tests ◽  
Control Group ◽  
Ige Receptor ◽  
Positive Skin ◽  
Ige Antibodies ◽  
Total Ige ◽  
Atopic Bronchial Asthma ◽  
Ige Receptors ◽  
High Level

The purpose of the work is clinical and laboratory examination of children with an eosinophilic phenotype of bronchial asthma. Materials and methods. 160 children aged 5 to 18 years were examined: 130 patients with atopic bronchial asthma, 30 children of the control group. During the work, the level of eosinophils, the level of eosinophils carrying FcεRI+ and CD23+IgE+ receptor, the level of total IgE, IgE antibodies to domestic and epidermal allergens in the blood serum were determined, clinical and anamnestic data were studied. Results. In children with atopic bronchial asthma, the relative and absolute content of eosinophils, as well as eosinophils carrying FcεRI+ and CD23+IgE+ receptors, is statistically significantly higher than in the control group (p<0.001). In 71 (54.62%) children with bronchial asthma, eosinophilia was detected, with 58 (81.69%) patients having a high level of eosinophils bearing the FcεRI + receptor and 56 (78.87%) children with a high level of eosinophils carrying CD23+IgE+ receptor. In the group of children with bronchial asthma, a correlation was established between the level of total IgE and the level of eosinophils carrying FcεRI+ (r=+0.389; p<0.01) and CD23+IgE+ receptors (r=+ 0.677; p<0.0001). In children with eosinophilia, in most cases, the hereditary nature of the disease is observed, the first manifestations of allergy are already present in the first year of life, concomitant allergopathology has been established. The optimal effect in the treatment of patients with eosinophilia was obtained with the appointment, as a basic therapy, of inhaled glucocorticosteroids, as well as short-acting β2-agonists for arresting episodes of bronchial obstruction. In most patients with asthma who have eosinophilia, serum levels of IgE antibodies to Dermatophagoides pteronyssinus, to the coat of the cat and the dog's coat are revealed, and positive skin scarification tests with these allergens are detected. Conclusion. In children with atopic bronchial asthma, there are signs of an eosinophilic phenotype: eosinophilia, high expression of FcεRI+ and CD23+IgE+ receptors, increased levels of total IgE and IgE antibodies, positive skin tests with domestic and epidermal allergens, hereditary predisposition, effectiveness of inhaled glucocorticosteroids.

STUDY OF THE VALUE OF BACTERIAL VACCINES IN THE TREATMENT OF BRONCHIAL ASTHMA ASSOCIATED WITH RESPIRATORY INFECTIONS

PEDIATRICS ◽  
1959 ◽  
Vol 24 (3) ◽  
pp. 427-433 ◽  
Author(s):  
Douglas E. Johnstone
Keyword(s):  
Bronchial Asthma ◽  
Respiratory Infections ◽  
Maximum Dose ◽  
Treated Group ◽  
Infants And Children ◽  
Skin Tests ◽  
Controlled Study ◽  
Control Group ◽  
Positive Skin ◽  
Number Of Children

A controlled study of the value of bacterial vaccines in 118 infants and children subject to repeated episodes of asthma associated with respiratory infections is described. Both vaccine-treated and a control group, treated similarly except for omission of bacterial vaccines from hyposensitization therapy, were compared before and at the end of a 3½-year period. When the maximum dose of vaccine was administered every 28 days, no statistically significant differences were observed between the two groups with regard to the following criteria: 1) number of asthmatic episodes per year; 2) average number of days wheezing per year; 3) average number of days school missed per year during asthmatic episodes; 4) number of children with 100%, 50-100% or 0-50% reduction in number of asthma attacks per year. In the vaccine-treated group the type of vaccine used did not appear to influence the degree of improvement as reflected in number of asthmatic attacks per year in the last year of the study compared to the year preceding the study. The incidence of positive skin tests to stock bacterial vaccines and a commercially prepared vaccine was approximately the same in both groups.

THE RELATIONSHIP BETWEEN PARENTAL AND CHILDREN'S SERUM IMMUNOGLOBULIN E (IgE) AND ASTHMA

PEDIATRICS ◽  
1996 ◽  
Vol 98 (2) ◽  
pp. 329-329
Author(s):  
Stanley P. Galant
Keyword(s):  
Bronchial Asthma ◽  
Immunoglobulin E ◽  
Skin Tests ◽  
Z Score ◽  
Significant Determinant ◽  
Positive Skin ◽  
Asthmatic Children ◽  
Children With Asthma ◽  
Z Scores ◽  
The Relationship

Purpose of the Study. To examine parental factors that influence bronchial asthma and immunoglobulin E (IgE) levels in their children. Methods. The data in this study were derived from the Tucson Epidemiological Study of Airways Obstructive Diseases since 1972. Eleven evaluation periods have been performed since that time. Asthma histories were established by questionnaire. IgE levels were obtained in 738 children, 1043 fathers, and 1261 mothers. IgE levels were determined by paper radioimmunosorbent (PRIST) technique. IgE Z scores were established. An IgE Z score is the number of standard deviations IgE differs from matched age and sex-matched subjects and log corrected. Skin tests were performed by prick technique to house dust, mold, grass, tree and weed mix and appropriate controls. Results. The percentage of children with bronchial asthma increased with the presence of asthma in parents so that 11.5% of children with asthma occurred in families with no parental asthma compared with 48.6% when both parents had asthma. The rate of childhood bronchial asthma was significantly related to parental IgE levels only when the mother and father had bronchial asthma (43% vs 20%) and much less so (22% vs 10%), when there was no parental asthma. In addition, asthma in the child proved to be a highly significant determinant of the child's IgE Z score, even after correcting for parental IgE Z score. The presence of atopy (ie, positive skin tests) was also not a significant determinant of IgE levels in asthmatic children. Discussion. The authors conclude that the inheritance of IgE is only one factor related to the development of asthma, and is limited as a predictor.

scholarly journals Serum levels of total IgE and soluble CD23 in bronchial asthma

1996 ◽  
Vol 5 (1) ◽  
pp. 43-46 ◽  
Author(s):  
G. Di Lorenzo ◽  
P. Mansueto ◽  
M. Melluso ◽  
G. Morici ◽  
D. Cigna ◽  
...  
Keyword(s):  
Bronchial Asthma ◽  
Healthy Subjects ◽  
Serum Levels ◽  
Total Serum ◽  
Control Group ◽  
Bronchial Responsiveness ◽  
Total Ige ◽  
Soluble Cd23 ◽  
Asthmatic Patients ◽  
Normal Controls

The aim of the present study was to compare, during the pollen season, serum levels of total IgE and soluble CD23 (sCD23) from patients with allergic bronchial asthma, with those from healthy subjects. Significantly higher levels of total IgE and sCD23 were found in patients with asthma compared to the control group. Both in normal controls and in asthmatic patients, a significant correlation was shown between the levels of these two molecules. In asthmatic patients, significant correlations were found for both total IgE and sCD23, with lung function measured as bronchial responsiveness to inhaled methacholine. These results suggest that in asthmatic patients, in addition to the study of total serum IgE levels, the assessment of sCD23 serum levels may be helpful in the evaluation of disease activity.

scholarly journals Analysis of associations of polymorphisms in the genes coding for L4, IL10, IL13 with the development of atopic bronchial asthma and its remission

2019 ◽  
pp. 87-91
Author(s):  
Yu.V. Zhorina ◽  
S.O. Abramovskikh ◽  
G.L. Ignatova ◽  
O.G. Ploshchanskay
Keyword(s):  
Bronchial Asthma ◽  
Molecular Mechanisms ◽  
Control Group ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Atopic Bronchial Asthma ◽  
Genotype Frequencies ◽  
Urban Sample ◽  
Allele Specific ◽  
Polymerase Chain

Bronchial asthma is a multifactorial disease underpinned by chronic inflammation. The atopic phenotype of BA implies the presence of similar molecular mechanisms of pathogenesis between the patients. The aim of this study was to analyze the associations between the development of atopic BA/its remission and the following polymorphisms of interleukin genes: IL4 (rs2243250; C-589T), IL10 (rs1800896; G-1082A; rs1800872; C-592A), and IL13 (rs20541; Arg130Gln). Using allele-specific polymerase chain reaction (PCR), we studied the listed SNPs in the mixed urban sample of patients with BA (n = 53) and the controls (n = 30) residing in South Ural. The analysis revealed that genotype АА of IL10 (rs1800872) occurred more frequently in the control group (23.3%) than in the patients with atopic BA (5.7%) (OR = 0.197; 95% CI [0.047–0.832]; р = 0.031). No differences in genotype frequencies were observed between the patients with atopic BA and the controls for other studied polymorphisms. Our study failed to demonstrate the association of the listed polymorphisms and BA remission.

IgE LEVELS IN SERA OF CHILDREN WITH ASTHMA

PEDIATRICS ◽  
1971 ◽  
Vol 47 (5) ◽  
pp. 848-856
Author(s):  
Lata Kumar ◽  
Richard W. Newcomb ◽  
Kimishige Ishizaka ◽  
Elliott Middleton ◽  
Margaret M. Hornbrook
Keyword(s):  
Atopic Dermatitis ◽  
Bronchial Asthma ◽  
Corticosteroid Therapy ◽  
Systemic Corticosteroid ◽  
Skin Tests ◽  
Positive Skin ◽  
Children With Asthma ◽  
Severity Of The Disease ◽  
Systemic Corticosteroid Therapy

Concentrations of the immunoglobulin that is associated with reaginic activity, IgE (γE), were measured in sera of 116 children with bronchial asthma. High γE (&gt;700 ng/ml) were found in only 30. High γE levels were found in children with many positive skin tests (P &lt; 0.01) and with active atopic dermatitis (P &lt; 0.01). Long term, systemic corticosteroid therapy was found to be associated with normal γE values (P &lt; .001) even when the characteristics of the disease would otherwise have indicated high values. As no correlation is found to be evident between severity of the disease and γE levels, it may be suggested that corticosteroids may decrease intravascular γE. The serum γE values failed to reveal two or more clinically different groups among our patients.

scholarly journals PHENOTYPES OF EOSINOPHILS IN THE DIAGNOSIS OF ATOPIC BRONCHIAL ASTHMA IN CHILDREN

2019 ◽  
Vol 29 (3) ◽  
pp. 79-83
Author(s):  
Elena Asiryn ◽  
Pavel Novikov ◽  
Volha Matsiushchanka ◽  
Laimutė Vaidelienė ◽  
Goda Misevičiūtė ◽  
...  
Keyword(s):  
Bronchial Asthma ◽  
Allergic Diseases ◽  
Control Group ◽  
Healthy Children ◽  
Absolute Level ◽  
Relative Level ◽  
Relative Index ◽  
Asthmatic Children ◽  
Atopic Bronchial Asthma ◽  
The Absolute

The aim of the research was to study the phenotype of eosinophils in children with atopic bronchial asthma and their role as diagnostic criteria of the disease. Materials and methods. We have examined 130 children aged from 6 to 18 years old with atopic bronchial asthma. The control group consisted of 40 healthy children from 6 to 18 years old, who didn’t have allergic diseases. During the research the relative and absolute levels of eosinophils, the level of eosinophils carrying FcεRI and FcεRII (CD23) – receptors were established. Results. The relative level of eosinophils in asthmatic children was 6.00% [3.00; 8.00], the absolute level – 417.00 cells/μL [232.00; 636,00], which is significantly higher compared to control group, where the relative level was 2.50% [1.00; 3.00%] and the absolute level was 166.50 cells/μl [86.00; 213.00] (p&lt;0.001). The level of eosinophils carrying the FcεRI receptor was 420,00 cells/μl [250,00; 660,00], which is significantly higher than in the control group – 200,00 cells/μl [140,00; 240.00] (p&lt;0.001). The relative level of eosinophils carrying CD23 receptor on their surface was 62.20% [35.40; 76.60] and the absolute level –223.37 cells/μl [105.30; 375.24]. The results are significantly higher than those of the control group: 25.45% [14.30; 30,60] (p&lt;0,001), and 30,88 cells/μl [25,63; 42.84] (p &lt;0.0001) respectively. It was found in the ROC-analysis that there is high evidence of presence of atopic bronchial asthma if absolute levels of eosinophils carrying CD23 receptor was 73.008 cells/μl or more or the relative index – 35.10% and more. Conclusions. The relative and absolute levels of eosinophils, as well as eosinophils carrying FcεRI and CD23 receptors are statistically significantly higher in atopic bronchial asthma than in control group patients. Increased number or relative index (73.008 cells/μl or ≥ 35.10% respectively) of eosinophils carrying CD 23 receptors can be considered as a diagnostic criterion of atopic bronchial asthma.

scholarly journals The efficiency of non-specific inhaled immunotherapy with ruzam in patients with atopic bronchialasthma

2011 ◽  
Vol 8 (4) ◽  
pp. 49-54
Author(s):  
D V Terekhov ◽  
Nataliya Mikhaylovna Nenasheva ◽  
D V Terekhov ◽  
N M Nenasheva
Keyword(s):  
Blood Serum ◽  
Bronchial Asthma ◽  
Bronchial Hyperresponsiveness ◽  
Allergic Inflammation ◽  
Induced Sputum ◽  
Control Group ◽  
Asthma Control Test ◽  
Open Label ◽  
Atopic Bronchial Asthma ◽  
Markers Of Inflammation

Background. To study the efficacy and safety of inhaled form of ruzam in adult patients with persistent mild and moderate atopic bronchial asthma (ba). Materials and methods. a placebo-controlled prospective, randomized, open label study of an efficacy of ruzam a solution for inhalations (in bottles on 2,5 or 5 ml) on 2,5 ml onсe a day through nebulizer during 2 weeks was conducted in adult asthmatic patients. The efficacy of therapy was evaluated by dynamics of symptoms and requirement in ƒ2-agonists, besides PEfr, spirometry, bronchial hyperresponsiveness (bhr), asthma control test (aCT ), level of the markers of the allergic inflammation were performed. Results. reduction of bа symptoms, increase morning PEfr, decrease of bhr and NОex level in patients who received ruzam compared with control group was observed. achievement of clinical control of bа in patients treated by ruzam was accompanied by statistically significant positive dynamics of markers of inflammation (ifNg, ECP) in blood serum and in supernatant of the induced sputum. it testifies about anti-inflammatory influence of ruzam. Conclusion. inhaled therapy with ruzam in a complex with iNCs basic therapy is an effective and safe method of treatment of persistent atopic bronchial asthma. This combination allows to reach clinical and functional control of ba faster and to reduce inflammation markers in blood serum and induced sputum.

Short-Term Results of Combined Radioimmunotherapy in Inoperable Lung Cancer

1976 ◽  
Vol 62 (6) ◽  
pp. 587-597 ◽  
Author(s):  
Corrado Gallo Curcio ◽  
Carla Vasile ◽  
Antonio Gianciotta ◽  
Alessandro Casali ◽  
Tommaso Gionfra ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Patients ◽  
Response To Therapy ◽  
Skin Tests ◽  
Delayed Type Hypersensitivity ◽  
Control Group ◽  
Short Term ◽  
Positive Skin ◽  
Lung Cancer Patients ◽  
Low Levels

Fifty-three inoperable lung cancer patients were treated with radiotherapy combined with immunostimulation with BCG. A good response was obtained in 30 patients (56 %); the results were not significantly different than those obtained with a control group of 50 lung cancer patients matched by age, sex and stage of the disease (24 out of 50 = 46 %). These short-term results were compared with the immunologic « status » of patients evaluated before treatment by the parameters monitoring in vivo and in vitro delayed type hypersensitivity. The good response to therapy was documented in a higher percentage of patients with positive skin tests to recall and standard antigens and with normal values of lymphocyte transformation with PHA and Rosette E-forming cells, in comparison with patients with low levels of immunocompetence. An impairment of the cell-mediated immune response was found after combined therapy, presumably due to radiotherapy. BCG was not able to restore the patient's immunocompetence, no effect on host's immune reactivity was demonstrated. As regards humoral immunity, the patients with low levels of IgG before treatment (12/14) showed a good response.

scholarly journals Initial vegetative tone in mild atopic asthma in children

2021 ◽  
Vol 12 (2) ◽  
pp. 13-18
Author(s):  
Vladimir N. Buryak ◽  
Tatyana I. Antonova ◽  
Maria V. Dudko ◽  
Inna V. Malysheva ◽  
Kirill K. Shepelenko
Keyword(s):  
Nervous System ◽  
Autonomic Nervous System ◽  
Bronchial Asthma ◽  
The Body ◽  
Atopic Asthma ◽  
World Health ◽  
Control Group ◽  
Healthy Children ◽  
Autonomic Nervous ◽  
Atopic Bronchial Asthma

Background. In most industrialized countries, allergic diseases affect up to 20% of the population. This pathology belongs to the most common in children: according to the World Health Organization, more than 15% of the world's child population suffers from it. In recent years, there has been a significant increase in the frequency and more severe course of these diseases, in connection with which they are considered in modern society as a major medical and social problem. Thus, the prevalence of bronchial asthma, according to domestic and foreign authors, ranges from 0.2 to 8.1%. Purpose. In order to clarify the role of the autonomic nervous system in the genesis of the mild course of atopic bronchial asthma in childhood, the features of the interaction of the sympathetic and parasympathetic divisions of the autonomic nervous system in the examined children were clarified. Materials and methods. 126 children aged 10 to 14 years were examined. Atopic bronchial asthma was diagnosed in 91 children. At the same time, 61 of them were diagnosed with an intermittent course, 30 a mild persistent course of the disease. The control group consisted of 35 healthy children also aged 10 to 14 years. Complaints, anamnesis data were studied in all children, an objective and generally accepted laboratory and instrumental examination was carried out. All examined children underwent daily monitoring of the electrocardiogram, according to the results of which, based on the analysis of time and frequency indicators of heart rate variability, a variant of the initial autonomic tone was established. Results. In children with atopic bronchial asthma, both with intermittent and mild persistent course, an absolute or relative dominance of sympathetic influences was revealed against the background of varying degrees of decrease in parasympathetic activity, which was interpreted as a compensatory reaction of the body in response to chronic allergic inflammation.

scholarly journals Determination of basophil activation and IgE antibodies to the components of dental materials in cases of intolerance to dental prosthetics

2017 ◽  
pp. 65-73
Author(s):  
I Karpuk ◽  
D Novikov
Keyword(s):  
Dental Materials ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Metal Salts ◽  
Control Group ◽  
Diagnostic Efficiency ◽  
Positive Skin ◽  
Ige Antibodies ◽  
Skin Hypersensitivity ◽  
Dental Prosthetics

The aim of the study was to determine the activation of blood basophils and IgE antibodies to the components of dental materials (CDM) in patients with intolerance to dental prosthetics. Materials and methods: we examined 22 patients with positive skin application tests (AT) with metal salts solutions (NiCl2 (3%), CrCl3 (3%), CoCl2 (1%) and complaints of intolerance to dental materials (IDM), and 21 patients without IDM complaints. We determined the levels of CD203c+CD63+ basophils after incubation of blood with incubation liquid containing particles of prosthetics (LPP) and metal salts solutions (MSS): NiCl2, CrCl3, CoCl2, ZnCl2, MnCl2, also measured IgE antibodies to these salts using ELISA. Results: in patients with IDM and skin hypersensitivity to metal salts an increase or decrease of CD203c+basophils after incubation of basophils with LPP and MSS occurred. In patients with skin hypersensitivity to CDM basophils significantly more often and stronger reacted with LPP and metal salts compared to basophils of control group patients. It was established that LPP had the highest diagnostic efficiency (sensitivity 72,7%; specificity 90,5%; AUC 0,86; p=0,0003; with threshold of expression growth for CD203c+63+ basophils equaling 16,68%), compared to MSS as basophil activation inductor. Conclusion: basophil activation test and IgE antibodies determination are recommended for detection of hypersensitivity and allergy in patients with IDM.

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