scholarly journals Canine Adipose-Derived Stem Cell Aggregates as a Viable Substitute to Actual Canine Dermal Papillae

2015 ◽  
Vol 38 (2) ◽  
pp. 167-173 ◽  
Author(s):  
Sohee Bae ◽  
Jina Kim ◽  
Li Li ◽  
Aeri Lee ◽  
Hyunjoo Lim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hair Follicle ◽  
Dermal Papilla ◽  
Human Medicine ◽  
Pcr Analysis ◽  
Molecular Characteristics ◽  
Dermal Papilla Cells ◽  
Dermatological Disease ◽  
Adipose Derived Stem Cell

Abstract Hair loss is a major dermatological disease in veterinary and human medicine. Active studies on hair regeneration with mesenchymal stem cells have been performed in an effort to solve the limitations of conservative treatments in human medicine. Our understanding of the canine hair follicle (HF), considering a useful model for the study of the human alopecia, is limited. This study was designed to broaden our understanding of canine dermal papilla (DP), and to reconstruct dermal papilla-like tissue (DPLT) using canine adipose-derived mesenchymal stem cells (AD-MSCs), as an alternative to actual DP. We cultured canine DPs, observed their culture patterns and compared their expression level of DP-related genes and proteins with those of DPLTs by performing RT-PCR analysis and Western blotting. Canine dermal papilla cells (DPCs) showed multilayer culture patterns with pseudo-papillae. Reconstruction of DPLTs was performed successfully. Not only were they morphologically similar to actual DPs, but we also observed similarities between DPCs and DPLTs in molecular characteristics. These findings suggested that DPLT was a viable substitute for DP. This study will not only be helpful for understanding the morphological and molecular characteristics of canine DPCs, but may also serve as a basis for understanding human hair follicle biology and potential therapeutic strategies for alopecia.

Androgens downregulate BMP2 impairing the inductive role of dermal papilla cells on hair follicle stem cells differentiation

2021 ◽  
Vol 520 ◽  
pp. 111096
Author(s):  
Julieta María Ceruti ◽  
Florencia Maia Oppenheimer ◽  
Gustavo José Leirós ◽  
María Eugenia Balañá
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Dermal Papilla ◽  
Dermal Papilla Cells ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

scholarly journals Single Transcription Factor Reprogramming of Hair Follicle Dermal Papilla Cells to Induced Pluripotent Stem Cells

Stem Cells ◽  
2011 ◽  
Vol 29 (6) ◽  
pp. 964-971 ◽  
Author(s):  
Su-Yi Tsai ◽  
Britta Am Bouwman ◽  
Yen-Sin Ang ◽  
Soo Jeong Kim ◽  
Dung-Fang Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factor ◽  
Induced Pluripotent Stem Cells ◽  
Hair Follicle ◽  
Pluripotent Stem Cells ◽  
Dermal Papilla ◽  
Dermal Papilla Cells ◽  
Induced Pluripotent

scholarly journals Reprogramming of Human Hair Follicle Dermal Papilla Cells into Induced Pluripotent Stem Cells

2012 ◽  
Vol 132 (6) ◽  
pp. 1725-1727 ◽  
Author(s):  
Claire A. Higgins ◽  
Munenari Itoh ◽  
Keita Inoue ◽  
Gavin D. Richardson ◽  
Colin A.B. Jahoda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Hair Follicle ◽  
Pluripotent Stem Cells ◽  
Human Hair ◽  
Dermal Papilla ◽  
Human Hair Follicle ◽  
Dermal Papilla Cells ◽  
Induced Pluripotent

Treatment of alopecia by transplantation of hair follicle stem cells and dermal papilla cells encapsulated in alginate gels

2008 ◽  
Vol 70 (5) ◽  
pp. 1014-1016 ◽  
Author(s):  
Jie Zhao ◽  
Liang-Qi Liu ◽  
Ying-Juan Wang ◽  
Wei Yang ◽  
Wen-Xin Geng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Dermal Papilla ◽  
Dermal Papilla Cells ◽  
Hair Follicle Stem Cells ◽  
Alginate Gels ◽  
Follicle Stem Cells

Hair follicle stem cells? A distinct germinative epidermal cell population is activated in vitro by the presence of hair dermal papilla cells

1991 ◽  
Vol 99 (2) ◽  
pp. 373-385 ◽  
Author(s):  
A.J. Reynolds ◽  
C.A. Jahoda
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Dermal Papilla ◽  
Epidermal Cells ◽  
Dermal Papilla Cells ◽  
Germinative Cell ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

Germinative epidermal cells in the lower end bulb region of anagen hair follicles are highly active, and give rise to hair fibres through rapid proliferation and complex differentiation. They have often been termed hair follicle stem cells, but owing to difficulties in isolation and identification their properties have previously only been clearly documented in vivo. We aimed to isolate and culture germinative cells in vitro, and used microdissection methods to dissect a small but identifiable group of cells from complete follicles. Transmission electron microscopy confirmed that the isolated cells were identical to germinative epidermal cells in situ. SDS-PAGE was used to show that they did not have the same protein composition as epidermis from their immediate proximity (overlying hair matrix), or from other follicular (outer root sheath) and interfollicular (skin basal) regions. Moreover, the germinative cells were found to display morphology and in vitro behaviour that distinguished them from comparative epidermal cells. When cultured in media and on substrata normally conducive to epidermal cell growth they remained in a quiescent state, and did not divide or differentiate. In contrast to other epidermal cells that formed typical pavement-like arrangements, germinative cells remained uniformly small, round and closely packed. However, when cultured in association with hair follicle dermal papilla cells they were radically stimulated into proliferative and aggregative behaviour. Furthermore, they were able to form organotypic-like structures, and exceptionally for skin-derived cell recombinations, a distinct basal lamina at the papilla-germinative cell junction. These results provide evidence that hair follicle germinative cells have intriguing properties that distinguish them from other follicular epidermis. The finding that they can be activated by dermal papilla cells reflects the intimate nature of the papilla-germinative cell relationship in situ, and should facilitate research into hair growth control mechanisms. The nature of germinative cells is discussed in the wider context of hair follicle stem-cell terminology.

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