IDENTIFICATION OF UTERIN MILK PROTEIN (UTMT) GENE IN BALI CATTLE  USING DIRECT SEQUENCING

The objective of this research was to identify diversity of exon 5 UTMP gene fragment in Bali cattle using direct sequencing. The total 60 blood samples of Bali Cattle derived from BPTU Bali in Bali siland (20 heads), BPTU Serading in Sumbawa island (20 heads) and Village Breeding Center in Barru District South Sulawesi (20 heads) were used to evaluate their genetic diversity at exon 5 UTMP gene. The forward and reverse data sequences were analyzed using Bioedit program and alignment analysis was carried out using MEGA5 program. Meanwhile haplotype analysis was performed by DnaSPv5 program. The result showed that partial sequences in exon 5 UTMP gene had 16 haplotypes with the highest number of haplotypes ware found in VBC Barru district South Sulawesi (8 haplotypes). Moreover, the highest average of haplotype (h) and nucleotide (p) diversity were found in VBC Barru district South Sulawesi were 0.7949 and 0.0016, respectively. In addition, minisatellite insersion was found in exon 5 UTMP gene fragment on Bali cattle which are consist of 5'-CCA GTC ATG AAG AAG GCA GAG GTC GTC GTG CCG GCG AAA-3'. According to our results, haplotype and minisatellite variation in exon 5 UTMP gene fragment can be used as a candidate genetic marker specific for reproductive trait in the Bali cattle and for its strategy breeding program in the future.

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Genetic polymorphism of MHC DRB3 (Major Histocompatibility Complex) of Bali cattle at Maiwa Breeding Center, South Sulawesi Indonesia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/247/1/012037 ◽

2019 ◽

Vol 247 ◽

pp. 012037

Author(s):

M I A Dagong ◽

S Baba ◽

L Rahim ◽

S R Aprilita Bugiwati ◽

M F Saade ◽

...

Keyword(s):

Major Histocompatibility Complex ◽

Genetic Polymorphism ◽

Major Histocompatibility ◽

South Sulawesi ◽

Histocompatibility Complex ◽

Breeding Center ◽

Bali Cattle

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Analysis of factors affecting to the income of Bali cattle farmers in Barru Regency, South Sulawesi Province, Indonesia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/788/1/012201 ◽

2021 ◽

Vol 788 (1) ◽

pp. 012201

Author(s):

Indrawirawan ◽

B Suwignyo ◽

T A Kusumastuti

Keyword(s):

Factors Affecting ◽

South Sulawesi ◽

Bali Cattle

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Association Between SSCP Haplotypes at the Bovine Growth Hormone Gene and Milk Protein Percentage

Genetics ◽

10.1093/genetics/142.3.945 ◽

1996 ◽

Vol 142 (3) ◽

pp. 945-951

Author(s):

A Lagziel ◽

E Lipkin ◽

M Soller

Keyword(s):

Growth Hormone ◽

Candidate Gene ◽

Milk Protein ◽

Haplotype Analysis ◽

Bos Taurus ◽

Bovine Growth Hormone ◽

Growth Hormone Gene ◽

Protein Percentage ◽

Quantitative Genetic ◽

Positive Effect

Abstract The bovine Growth Hormone gene (bGH) is an attractive candidate gene for milk production in cattle. Single-strand conformation polymorphisms at bGH were identified and used to define haplotype configurations at this gene in the Israeli Holstein dairy cattle population (Bos taurus) and in the parent animals of the International Bovine Reference Family Panel (a collection of B. taurus and B. indicus crosses). B. taurus and B. indicus haplotypes at the bGH gene differed qualitatively, confirming the previously proposed long evolutionary separation of these cattle subraces. Only a small number of bGH haplotypes were present in the Israel Holstein population. One of the haplotypes, apparently of B. indicus origin, was found to have a highly significant positive effect on milk protein percentage. This illustrates the utility of the haplotype approach for uncovering candidate gene involvement in quantitative genetic variation in agricultural populations. The strong effect of an indicine haplotype in a taurine background raises the possibility that indicine alleles at other candidate genes may comprise a genetic resource for improvement of taurine populations. It is proposed that haplotype analysis may be a useful adjunct to measures of genetic distance for evaluating rare breeds with respect to gene conservation.

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Distribution and density of Bali Cattle in South Sulawesi Province

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/492/1/012155 ◽

2020 ◽

Vol 492 ◽

pp. 012155

Author(s):

I M Saleh ◽

S Nurlaelah ◽

A Asnawi ◽

M Aminawar

Keyword(s):

South Sulawesi ◽

Bali Cattle

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Identifikasi Keragaman Gen FTO Pada Bangsa Sapi Potong Indonesia

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v6i2.5655 ◽

2019 ◽

Vol 6 (2) ◽

pp. 232

Author(s):

Sutikno Sutikno ◽

Rudy Priyanto ◽

Cece Sumantri ◽

Jakaria Jakaria

Keyword(s):

Beef Cattle ◽

Genetic Markers ◽

Direct Sequencing ◽

Nucleotide Position ◽

Fto Gene ◽

Blood Samples ◽

Pcr Rflp ◽

Gene Functions ◽

Hardy Weinberg Equilibrium ◽

Cattle Blood

ABSTRAK Gen FTO berfungsi sebagai regulasi homeostasis, deposisi lemak dan pengaturan obesitas. Penelitian ini bertujuan untuk mengidentifikasi polimorfisme SNP g.125550A>T di ekson 3 gen FTO pada bangsa sapi potong Indonesia. Sampel darah diperoleh dari 209 ekor sapi, terdiri atas sapi bali (44), madura (20), Pesisir (20), katingan (20), Peranakan ongole (PO) (22), Pasundan (20), Sumba Ongole (SO) (11), brahman (20), simental (15), dan limousin (18). Polimorfisme gen FTO dianalisis menggunakan metode PCR-RFLP (HpyCH4III) dan direct sequencing. Hasil genotiping SNP g.125550A>T adalah polimorfik (genotipe AA, AT, dan TT) pada sapi madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, dan limousin. Frekuensi alel A dan T masing-masing adalah 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 dan 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31. Nilai Ho dan He masing-masing adalah 0,60-0,14 dan 0,44-0,18 serta dalam keseimbangan Hardy-Weinberg (P>0.05). Sementara pada sapi bali bersifat monomorfik hanya bergenotipe AA. Hasil sekuensing SNP g.125550A>T ditemukan mutasi tranvesi A menjadi T pada posisi nukleotida g.125550. Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa SNP 125550A>T gen FTO beragam dan berpotensi dijadikan marka genetik untuk kualitas daging pada bangsa sapi potong Indonesia.Kata Kunci: gen FTO, PCR-RFLP, Sapi, SNP g.125550A>TABSTRACTThe FTO gene functions as regulation of homeostasis, fat deposition and regulation of obesity. This study aimed to identify the polymorphism of SNP g.125550A>T in exon 3 of FTO gene in Indonesian beef cattle. Blood samples were collected from 209 cattle, including bali (44), madura (20), pesisir (20), katingan (20), PO (22), pasundan (20), SO (11), brahman (20), simental (15), and limousin (18). Polymorphism of the FTO gene was analyzed using PCR-RFLP (HpyCH4III) and direct sequencing methods. The results of genotyping SNP g.125550A>T was polymorphic (AA, AT and TT genotypes) in madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, and limousin cattle. The frequency of A and T alleles were 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 and 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31 respectively. The values of Ho and He were 0,60-0,14 and 0,44-0,18 respectively and in Hardy-Weinberg equilibrium (P>0,05). While in Bali cattle was monomorphic (AA genotype). Results of sequencing SNP g.125550A>T of the FTO gene found a transverse mutation A to T at the nucleotide position g.125550. As a result of this study, it can be concluded that SNP 125550A>T of the FTO gene was diverse and potentially used as genetic markers for meat quality in Indonesian beef cattle.Keywords: cattle, FTO gene, PCR-RFLP, SNP g.125550A>T.

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Prevalence of Brucella abortus antibody in serum of Bali cattle in South Sulawesi

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/492/1/012095 ◽

2020 ◽

Vol 492 ◽

pp. 012095

Author(s):

K I Prahesti ◽

R Malaka ◽

F N Yuliati

Keyword(s):

Brucella Abortus ◽

South Sulawesi ◽

Bali Cattle

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Application of capital social of Bali cattle farmers that participate in the partnership system in Barru Regency, South Sulawesi Province

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/157/1/012059 ◽

2018 ◽

Vol 157 ◽

pp. 012059

Author(s):

S N Sirajuddin ◽

A R Siregar ◽

P Mappigau

Keyword(s):

Capital Social ◽

South Sulawesi ◽

Bali Cattle

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Polymorphism of ADIPOQ and EDG1 genes in Indonesian beef cattle

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.43.4.323-332 ◽

2018 ◽

Vol 43 (4) ◽

pp. 323

Author(s):

S. Sutikno ◽

R. Priyanto ◽

C. Sumantri ◽

J. Jakaria

Keyword(s):

Beef Cattle ◽

Promoter Region ◽

Marbling Score ◽

Blood Samples ◽

Adipoq Gene ◽

Pcr Rflp ◽

Hardy Weinberg Equilibrium ◽

Cattle Blood ◽

Bali Cattle ◽

Novel Snp

The ADIPOQ and EDG1 genes were responsible in intramuscular fat deposition and marbling scores. This study was aimed to identify polymorphism of indel g.81966364D>I in promoter region of ADIPOQ gene and SNP c.-312A>G in 5' UTR of EDG1 gene in Indonesian beef cattle. Blood samples were collected from 211 cattle, including Bali (44), Madura (20), Pesisir (18), Katingan (20), PO (22), Pasundan (20), SO (12), Brahman (20), Simmental (15) and Limousin (18). Polymorphism of ADIPOQ gene was analyzed using PCR and direct sequencingmethods, whereas EDG1 gene was analyzed using PCR-RFLP (MscI enzyme) and direct sequencingmethods. Results of genotyping indel g.81966364D>I was monomorphic (DD genotype). The SNP c.-312A>G was polymorphic (AA and AG genotype) in Madura, Pesisir, Pasundan, Brahman, and Limousine. The Frequencies of allele A and G were 0.95, 0.92, 0.98, 0.95, 0.94 and 0.05, 0.08, 0.02, 0.05, 0.06 respectively. The values of Ho and He were 0.05-0.17 and 0.05-0.15 respectively and in Hardy-Weinberg equilibrium (P>0.05). In Bali, Katingan, PO, SO and Simmental were monomorphic (GG genotype). In Bali cattle, two novel SNP candidates were found in position of c.-399C>T and c.-273C>G which were potential to be used as genetic markers of marbling score for Bali cattle. As result this study, it can be concluded that ADIPOQ gene was similar while EDG1 gene was different in Indonesian beef cattle. in addition, found two candidates potential SNP in Bali cattle.

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