Chromosome Banding Analysis of Peripheral Blood Lymphocytes Stimulated with IL-2 and CpG Oligonucleotide DSP30 in Patients with Chronic Lymphocytic Leukemia

Abstract Cell structures containing tubulin were studied in peripheral blood lymphocytes from 8 normal donors and 11 patients with CLL using specific antitubulin antibodies revealed by immunoperoxidase assay. The centriole and microtubules were clearly visible in both groups. A “nucleus-associated tubulin-containing structure” was revealed by antitubulin antibodies and was found in virtually all lymphocytes of normal subjects but in a considerably lower number of CLL lymphocytes. The nature of this structure and its relationship to other cell structures are discussed.

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Changes in Glycanic Determinants of Lymphocytes Membranes in Peripheral Blood in Patients with B-Cell Chronic Lymphocytic Leukemia under Antitumor Therapy

Ukraïnsʹkij žurnal medicini bìologìï ta sportu ◽

10.26693/jmbs06.06.141 ◽

2021 ◽

Vol 6 (6) ◽

pp. 141-147

Author(s):

G. S. Maslak ◽

◽

G. P. Chernenko ◽

V. M. Baibakov ◽

A. D. Viselko ◽

...

Keyword(s):

Chronic Lymphocytic Leukemia ◽

B Cell ◽

Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Lymphocytic Leukemia ◽

Cytostatic Therapy ◽

Tn Antigen ◽

Antitumor Therapy ◽

Blood Lymphocytes ◽

Cell Chronic Lymphocytic Leukemia

The purpose of the study was to study the nature of changes in the exposure of surface glycans of peripheral blood lymphocytes in patients with B-cell chronic lymphocytic leukemia under conditions of antitumor therapy. Materials and methods. We studied the features of exposure of surface glycotopes of peripheral blood lymphocytes in patients with B-cell chronic lymphocytic leukemia under conditions of antitumor therapy using a set of seven lectins labeled with FITC and monoclonal antibodies to Tn-antigen- FITC for the detection of Tn antigen and CD43 exposure on blood lymphocytes. Cytostatic therapy included cyclophosphamide, vincristine (oncovin), prednisolone. Data were recorded on a Beckman Coulter EPICS flow cytometer. The results were processed using FCS3 Express. Results and discussion. The number of lymphocytes of healthy donors with a positive reaction to ConA, PHA-L, SNA, MAA-II and α1-acid glycoprotein amounted to 16.0±3.0%, 23.0±2.3%, 15.0±1.5%, 25.0±1.8% and 15.0±1.3%, respectively. The number of LABA-, UEA I-positive lymphocytes was 0.90±0.03% and 2.9±0.2%, respectively, and there was no binding to antibodies to Tn- and CD43-antigens. In the blood of patients with chronic lymphocytic leukemia, the level of ConA-, SNA- and MAA-II-positive lymphocytes increased relative to control by 2.2, 3.7 and 2.6 times, respectively. The number of LABA- and UEA I-positive lymphocytes in patients with chronic lymphocytic leukemia increased by 11 (p <0.01) and 23 (p <0.001) times and amounted to 10.5±0.5% and 67.5±5.5% respectively. The number of lymphocytes with CD43 antigen on their surface increased by 72 times, and the Tn antigen increased by 80 times. Cytostatic therapy reduced the level of LABA- and UEA I-positive lymphocytes by almost half, and MAA II-positive cells and lymphocytes interacting with antibodies to CD43 and Tn antigen by a third. The level of PHA-L-positive lymphocytes in the blood of chronic lymphocytic leukemia patients after undergoing alkylating therapy increased by 18.0±2.0% and almost did not differ from those obtained in the control group. Conclusion. 1. In chronic lymphocytic leukemia patients, the structure of glycoconjugates in peripheral blood lymphocytes changes, manifested in increased exposure of L-fucose, α-mannose and N-acetylneuraminic acid, which is confirmed by a significant increase in relation to the control of the number of ConA-, SNA-, MAA-II-, LABA I-positive cells. 2. Patients with chronic lymphocytic leukemia showed a significant increase in the number of lymphocytes, in which the markers of carcinogenesis CD43 and Tn antigens were found. 3. Cytostatic therapy significantly reduced the level of LABA-, UEA I- and MAA II-positive cells, as well as partially Tn- and CD43-antigen-positive lymphocytes, which indicates its positive effect on the treatment of chronic lymphocytic leukemia

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Arginase activity alterations in peripheral blood lymphocytes in the human chronic lymphocytic leukemia.

Acta Biochimica Polonica ◽

10.18388/abp.1993_4879 ◽

1993 ◽

Vol 40 (1) ◽

pp. 160-163 ◽

Cited By ~ 1

Author(s):

L Konarska ◽

I Widzyńska ◽

H Zienkiewicz ◽

K Sułek

Keyword(s):

Chronic Lymphocytic Leukemia ◽

Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Lymphocytic Leukemia ◽

Arginase Activity ◽

Blood Lymphocytes

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Membrane difference in peripheral blood lymphocytes from patients with chronic lymphocytic leukemia and Hodgkin's disease.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.72.6.2428 ◽

1975 ◽

Vol 72 (6) ◽

pp. 2428-2432 ◽

Cited By ~ 19

Author(s):

U. Mintz ◽

L. Sachs

Keyword(s):

Chronic Lymphocytic Leukemia ◽

Peripheral Blood ◽

Hodgkin's Disease ◽

Hodgkin’S Disease ◽

Peripheral Blood Lymphocytes ◽

Lymphocytic Leukemia ◽

Blood Lymphocytes

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Visualization of tubulin in lymphocytes. I. Comparison of normal and chronic lymphocytic leukemia (CLL) lymphocytes

Blood ◽

10.1182/blood.v51.6.1031.bloodjournal5161031 ◽

1978 ◽

Vol 51 (6) ◽

pp. 1031-1037

Author(s):

G Dighiero ◽

E Karsenti ◽

JY Follezou ◽

M Bornens

Keyword(s):

Chronic Lymphocytic Leukemia ◽

Peripheral Blood ◽

Normal Subjects ◽

Peripheral Blood Lymphocytes ◽

Lower Number ◽

Lymphocytic Leukemia ◽

Blood Lymphocytes ◽

Cell Structures ◽

Immunoperoxidase Assay

Cell structures containing tubulin were studied in peripheral blood lymphocytes from 8 normal donors and 11 patients with CLL using specific antitubulin antibodies revealed by immunoperoxidase assay. The centriole and microtubules were clearly visible in both groups. A “nucleus-associated tubulin-containing structure” was revealed by antitubulin antibodies and was found in virtually all lymphocytes of normal subjects but in a considerably lower number of CLL lymphocytes. The nature of this structure and its relationship to other cell structures are discussed.

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Teucrium polium induces apoptosis in peripheral blood lymphocytes isolated from human chronic lymphocytic leukemia

Vojnosanitetski pregled ◽

10.2298/vsp181010005z ◽

2019 ◽

pp. 5-5

Author(s):

Milan Zaric ◽

Radica Zivkovic-Zaric ◽

Marina Mitrovic ◽

Ivana Nikolic ◽

Petar Canovic ◽

...

Keyword(s):

Chronic Lymphocytic Leukemia ◽

Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Lymphocytic Leukemia ◽

Blood Lymphocytes ◽

Teucrium Polium

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Chromosome banding analysis and genomic microarrays are both useful but not equivalent methods for genomic complexity risk stratification in chronic lymphocytic leukemia patients

Haematologica ◽

10.3324/haematol.2020.274456 ◽

2021 ◽

Author(s):

Silvia Ramos-Campoy ◽

Anna Puiggros ◽

Sílvia Beà ◽

Sandrine Bougeon ◽

María José Larráyoz ◽

...

Keyword(s):

Chronic Lymphocytic Leukemia ◽

Risk Stratification ◽

Chromosome Banding ◽

Risk Groups ◽

Lymphocytic Leukemia ◽

Genomic Complexity ◽

Genome Complexity ◽

Banding Analysis ◽

Chromosome Banding Analysis ◽

Prognostic Stratification

Genome complexity has been associated with poor outcome in patients with chronic lymphocytic leukemia (CLL). Previous cooperative studies established five abnormalities as the cut-off that best predicts an adverse evolution by chromosome banding analysis (CBA) and genomic microarrays (GM). However, data comparing risk stratification by both methods are scarce. Herein, we assessed a cohort of 340 untreated CLL patients highly enriched in cases with complex karyotype (CK, 46.5%) with parallel CBA and GM studies. Abnormalities found by both techniques were compared. Prognostic stratification in three risk groups based on genomic complexity [0-2, 3-4 and ≥5 abnormalities] was also analyzed. No significant differences in the percentage of patients classified into each category were detected, but only a moderate agreement was observed between methods when focusing in individual cases (κ=0.507; p

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HLA-DR-associated invariant chain is highly expressed in chronic lymphocytic leukemia

Blood ◽

10.1182/blood.v68.2.372.bloodjournal682372 ◽

1986 ◽

Vol 68 (2) ◽

pp. 372-377

Author(s):

F Narni ◽

J Kudo ◽

W Mars ◽

B Calabretta ◽

DL Florine ◽

...

Keyword(s):

Chronic Lymphocytic Leukemia ◽

Peripheral Blood ◽

Leukemic Cell ◽

Peripheral Blood Lymphocytes ◽

Lymphocytic Leukemia ◽

Invariant Chain ◽

Blood Lymphocytes ◽

Normal Tissues ◽

Hla Dr ◽

Leukocyte Populations

Total RNA extracted from peripheral blood lymphocytes of a patient with B-cell chronic lymphocytic leukemia (CLL) and the poly (A+) RNA was purified. A cDNA library was constructed and approximately 4,000 clones were screened in order to identify genes preferentially expressed in CLL. A relatively low repetition frequency characterizes the majority of the abundant mRNA species present in CLL lymphocytes. One clone, corresponding to the mRNA encoding the HLA-DR-associated invariant chain, was selected and its expression was examined in different leukemic cell populations and in normal tissues. DNA-RNA hybridization studies showed that the invariant chain mRNA (In-mRNA) is detectable in RNA preparations from human blood cells and their precursors, whereas no In-mRNA is found in several other tissues examined. Among various normal and leukemic leukocyte populations, the highest levels of In- mRNA are found in CLL. Therefore, a role of In-chain mRNA as a marker of CLL is proposed. Our data support a relationship between high levels of invariant chain mRNA and the out of cycle condition of CLL peripheral blood lymphocytes.

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