Prevalence ofChlamydia trachomatis,Neisseria gonorrhoeae,Mycoplasma genitalium,Ureaplasma urealyticumin Chronic Prostatitis Category IIIa and IIIb Patients Using Polymerase Chain Reaction

2013 ◽  
Vol 8 (2) ◽  
pp. 102 ◽  
Author(s):  
In-Chang Cho ◽  
Yoo Seok Kim ◽  
Sung Bin Kim ◽  
Soon Ki Kim ◽  
Gyeong In Lee ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Neisseria Gonorrhoeae ◽  
Chronic Prostatitis ◽  
Mycoplasma Genitalium ◽  
Chain Reaction ◽  
Ofchlamydia Trachomatis ◽  
Polymerase Chain

UriSwab: an effective transport medium for nucleic acid detection of Chlamydia trachomatis, Mycoplasma genitalium and Neisseria gonorrhoeae

Sexual Health ◽  
2017 ◽  
Vol 14 (6) ◽  
pp. 502 ◽  
Author(s):  
Anna-Maria G. Costa ◽  
Suzanne M. Garland ◽  
Rebecca Guy ◽  
Handan Wand ◽  
Sepehr N. Tabrizi
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Mixed Model ◽  
Mycoplasma Genitalium ◽  
Urine Samples ◽  
Sample Type ◽  
Chain Reaction ◽  
Increasing Trend ◽  
Polymerase Chain

Background Patient self-sampling allows for remote collection and return to clinic or laboratory by post. Urine samples, although convenient, are challenging to post. This study evaluated UriSwab (Copan, Brescia, Italy) as a collection and transport vessel for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Mycoplasma genitalium (MG) detection by polymerase chain reaction, compared with flocked swab and neat urine. Methods: Five replicates of each specimen type were prepared from previously characterised urine samples (n = 330), stored at room temperature (RT) or 37°C, then extracted on day 1, 3, 7, 10 and 16 (VERSANT kPCR Sample Prep System, Siemens, Munich, Germany). Crossing thresholds (Cq) from CT and NG detection (VERSANT CT/GC DNA 1.0 assay kit, Siemens) and MG detection (real-time polymerase chain reaction assay) were compared using logistic regression, stratified by sample type, temperature and analyte. Mixed-model statistical techniques were used to assess correlation between repeated observations. Results: UriSwab showed an increasing trend in Cq values at RT and 37°C for CT and NG, and RT for MG (all P < 0.01). UriSwab was not statistically significantly different to neat urine, except CT at RT (0.83, 95% confidence interval: 0.51–1.15). Flocked swab similarly showed increasing Cq values at 37°C for CT, a significant decreasing trend at RT for MG and increasing trend at 37°C for MG. Flocked swab was not statistically significantly different from neat urine at RT and 37°C for CT and MG. Conclusion: UriSwab allows transport of urine for CT, NG and MG detection regardless of storage time or temperature, suggesting that CT and NG are stable for up to 16 days and MG up to 10 days.

scholarly journals Detection of Mycoplasma genitalium from Male Patients with Non-Gonococcal Urethritis by Polymerase Chain Reaction

1994 ◽  
Vol 68 (11) ◽  
pp. 1376-1380
Author(s):  
Hisao KOMEDA ◽  
Takashi DEGUCHI ◽  
Mitsuru YASUDA ◽  
Kouji TADA ◽  
Hideki IWATA ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycoplasma Genitalium ◽  
Chain Reaction ◽  
Gonococcal Urethritis ◽  
Male Patients ◽  
Polymerase Chain

scholarly journals Adult gonococcal keratoconjunctivitis: early detection is key

2020 ◽  
Vol 17 (1) ◽  
pp. 30-34
Author(s):  
Daniel Lai ◽  
Keith Ong
Keyword(s):  
Polymerase Chain Reaction ◽  
Early Detection ◽  
Neisseria Gonorrhoeae ◽  
Ophthalmic Solution ◽  
Careful Monitoring ◽  
Corneal Ulceration ◽  
Chain Reaction ◽  
Bacterial Conjunctivitis ◽  
Polymerase Chain ◽  
The Right

We describe a case of a 52-year-old male presenting with severe mucopurulent conjunctivitis of the right eye. Corneal ulceration and associated anterior chamber activity was noted later in the course of the disease. Neisseria gonorrhoeae was positive on polymerase chain reaction (PCR) testing earlier than traditional microscopy and culture. He was successfully treated with ceftriaxone 500 mg intravenously and azithromycin 1 g orally as single doses in addition to ofloxacin ophthalmic solution 0.3% hourly to the right eye. This case highlights the need to consider the possibility of gonococcus in cases of suspected bacterial conjunctivitis, careful monitoring for corneal involvement and the importance of early detection with PCR.

scholarly journals Identification of Neisseria gonorrhoeae isolates with a recombinant porA gene in Scotland, United Kingdom, 2010 to 2011

2012 ◽  
Vol 17 (9) ◽  
Author(s):  
K Eastick ◽  
A Winter ◽  
S Jamdar
Keyword(s):  
Polymerase Chain Reaction ◽  
United Kingdom ◽  
Neisseria Gonorrhoeae ◽  
Sequence Type ◽  
The Other ◽  
Chain Reaction ◽  
Polymerase Chain

Three isolates of Neisseria gonorrhoeae have been identified in Scotland in 2010 and 2011, which lack sequences in the porA pseudogene commonly used as the target for confirmatory gonorrhoea polymerase chain reaction assays. Two isolates were clustered temporally and geographically and have the same sequence type and porA sequence. A similar strain was reported in Australia during early 2011. The other Scottish isolate was identified separately and is different in sequence type and porA sequence.

scholarly journals Detection of Neisseria gonorrhoeae in First-Voided Urine Sediments from Male Urethritis Patients by Polymerase Chain Reaction

1992 ◽  
Vol 66 (9) ◽  
pp. 1209-1212 ◽  
Author(s):  
Hisao KOMEDA ◽  
Takashi DEGUCHI ◽  
Hiroyuki YAMAMOTO ◽  
Hideki IWATA ◽  
Yasuhisa ITO ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Neisseria Gonorrhoeae ◽  
Chain Reaction ◽  
Polymerase Chain
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