scholarly journals In vivo Ca2+ dynamics induced by Ca2+ injection in individual rat skeletal muscle fibers

2017 ◽  
Vol 5 (5) ◽  
pp. e13180 ◽  
Author(s):  
Mario Wakizaka ◽  
Hiroaki Eshima ◽  
Yoshinori Tanaka ◽  
Hideki Shirakawa ◽  
David C. Poole ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
Rat Skeletal Muscle ◽  
Skeletal Muscle Fibers

Ischemia/reperfusion-induced changes in intracellular free Ca2+ levels in rat skeletal muscle fibers – an in vivo study

2000 ◽  
Vol 440 (2) ◽  
pp. 302-308 ◽  
Author(s):  
Tamás Ivanics ◽  
Zsuzsa Miklós ◽  
Zoltán Ruttner ◽  
Sándor Bátkai ◽  
Dick W. Slaaf ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Ischemia Reperfusion ◽  
Muscle Fibers ◽  
In Vivo Study ◽  
Rat Skeletal Muscle ◽  
Skeletal Muscle Fibers ◽  
Induced Changes

scholarly journals ROS-Mediated Decline in Maximum Ca2+-Activated Force in Rat Skeletal Muscle Fibers following In Vitro and In Vivo Stimulation

PLoS ONE ◽  
2012 ◽  
Vol 7 (5) ◽  
pp. e35226 ◽  
Author(s):  
Travis L. Dutka ◽  
Esther Verburg ◽  
Noni Larkins ◽  
Kristin H. Hortemo ◽  
Per K. Lunde ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
Rat Skeletal Muscle ◽  
Skeletal Muscle Fibers

Effects of Creatine Supplementation During Resistance Training on Myosin Heavy Chain (MHC) Expression in Rat Skeletal Muscle Fibers

2010 ◽  
Vol 24 (1) ◽  
pp. 88-96 ◽  
Author(s):  
Andreo F Aguiar ◽  
Danilo H Aguiar ◽  
Alan DS Felisberto ◽  
Fernanda R Carani ◽  
Rachel C Milanezi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Resistance Training ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Muscle Fibers ◽  
Creatine Supplementation ◽  
Rat Skeletal Muscle ◽  
Skeletal Muscle Fibers

Glycogen Depletion in Rat Skeletal Muscle Fibers During Exercise

1975 ◽  
pp. 397-401 ◽  
Author(s):  
R. B. Armstrong ◽  
C. W. Saubert ◽  
W. L. Sembrowich ◽  
R. E. Shepherd ◽  
P. D. Gollnick
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
Glycogen Depletion ◽  
Rat Skeletal Muscle ◽  
Skeletal Muscle Fibers

scholarly journals Defective Acetylcholine Receptor Subunit Switch Precedes Atrophy of Slow-Twitch Skeletal Muscle Fibers Lacking ERK1/2 Kinases in Soleus Muscle

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Shuo Wang ◽  
Bonnie Seaberg ◽  
Ximena Paez-Colasante ◽  
Mendell Rimer
Keyword(s):  
Skeletal Muscle ◽  
Acetylcholine Receptor ◽  
Soleus Muscle ◽  
Muscle Fibers ◽  
Neuromuscular Synapse ◽  
Fiber Morphology ◽  
Skeletal Muscle Fibers ◽  
Slow Twitch

Abstract To test the role of extracellular-signal regulated kinases 1 and 2 (ERK1/2) in slow-twitch, type 1 skeletal muscle fibers, we studied the soleus muscle in mice genetically deficient for myofiber ERK1/2. Young adult mutant soleus was drastically wasted, with highly atrophied type 1 fibers, denervation at most synaptic sites, induction of “fetal” acetylcholine receptor gamma subunit (AChRγ), reduction of “adult” AChRε, and impaired mitochondrial biogenesis and function. In weanlings, fiber morphology and mitochondrial markers were mostly normal, yet AChRγ upregulation and AChRε downregulation were observed. Synaptic sites with fetal AChRs in weanling muscle were ~3% in control and ~40% in mutants, with most of the latter on type 1 fibers. These results suggest that: (1) ERK1/2 are critical for slow-twitch fiber growth; (2) a defective γ/ε-AChR subunit switch, preferentially at synapses on slow fibers, precedes wasting of mutant soleus; (3) denervation is likely to drive this wasting, and (4) the neuromuscular synapse is a primary subcellular target for muscle ERK1/2 function in vivo.

Excitation-contraction latencies in postnatal rat skeletal muscle fibers

1970 ◽  
Vol 29 (1) ◽  
pp. 142-151 ◽  
Author(s):  
Edward R. Chaplin ◽  
George W. Nell ◽  
Sheppard M. Walker
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
Rat Skeletal Muscle ◽  
Skeletal Muscle Fibers

Characteristics of phosphate-induced Ca2+ efflux from the SR in mechanically skinned rat skeletal muscle fibers

2000 ◽  
Vol 278 (1) ◽  
pp. C126-C135 ◽  
Author(s):  
Adrian M. Duke ◽  
Derek S. Steele
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Muscle Fibers ◽  
Cyclopiazonic Acid ◽  
Creatine Phosphate ◽  
Ruthenium Red ◽  
Rat Skeletal Muscle ◽  
Atpase Inhibitor ◽  
Skeletal Muscle Fibers ◽  
Efflux Pathway

The effects of Pi on sarcoplasmic reticulum (SR) Ca2+ regulation were studied in mechanically skinned rat skeletal muscle fibers. Brief application of caffeine was used to assess the SR Ca2+ content, and changes in concentration of Ca2+([Ca2+]) within the cytosol were detected with fura 2 fluorescence. Introduction of Pi (1–40 mM) induced a concentration-dependent Ca2+ efflux from the SR. In solutions lacking creatine phosphate (CP), the amplitude of the Pi-induced Ca2+ transient approximately doubled. A similar potentiation of Pi-induced Ca2+ release occurred after inhibition of creatine kinase (CK) with 2,4-dinitrofluorobenzene. In the presence of ruthenium red or ryanodine, caffeine-induced Ca2+ release was almost abolished, whereas Pi-induced Ca2+ release was unaffected. However, introduction of the SR Ca2+ ATPase inhibitor cyclopiazonic acid effectively abolished Pi-induced Ca2+ release. These data suggest that Pi induces Ca2+ release from the SR by reversal of the SR Ca2+ pump but not via the SR Ca2+ channel under these conditions. If this occurs in intact skeletal muscle during fatigue, activation of a Ca2+efflux pathway by Pi may contribute to the reported decrease in net Ca2+ uptake and increase in resting [Ca2+].

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