Investigation of the low-dose γ-irradiation effect on the spontaneous and high-dose radiation-induced level of cytogenetic damage in mouse bone marrow cells in vivo

Genistein, a major phytoestrogen of soy, is considered a potential drug for prevention and treatment of postmenopausal osteoporosis. The aim of the present study was to compare the effects of genistein, estradiol and raloxifene on the skeletal system in vivo and in vitro. Genistein (5 mg/kg), estradiol (0.1 mg/kg) or raloxifene hydrochloride (5 mg/kg) were administered daily by a stomach tube to mature ovariectomized Wistar rats for 4 weeks. Bone mass, mineral and calcium content, macrometric parameters and mechanical properties were examined. Also the effects of genistein, estradiol and raloxifene (10(-9)-10(-7) M) on the formation of osteoclasts from neonatal mouse bone marrow cells and the activity of osteoblasts isolated from neonatal mouse calvariae were compared. In vivo, estrogen deficiency resulted in the impairment of bone mineralization and bone mechanical properties. Raloxifene but not estradiol or genistein improved bone mineralization. Estradiol fully normalized the bone mechanical properties, whereas genistein augmented the deleterious effect of estrogen-deficiency on bone strength. In vitro, genistein, estradiol and raloxifene inhibited osteoclast formation from mouse bone marrow cells, decreasing the ratio of RANKL mRNA to osteoprotegerin mRNA expression in osteoblasts. Genistein, but not estradiol or raloxifene, decreased the ratio of alkaline phosphatase mRNA to ectonucleotide pyrophosphatase phosphodiesterase 1 mRNA expression in osteoblasts. This difference may explain the lack of genistein effect on bone mineralization observed in ovariectomized rats in the in vivo study. Concluding, our experiments demonstrated profound differences between the activities of genistein, estradiol and raloxifene towards the osseous tissue in experimental conditions.

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Retraction: Stanimirovic Z, Aleksic N, Kulic M, Maletic M. Fumagillin-induced chromosome aberrations in mouse bone-marrow cells, Arch Biol Sci. 2010;62(1):47-55, DOI: 10.2298/ABS1001047S

Archives of Biological Sciences ◽

10.2298/abs150320032e ◽

2015 ◽

Vol 67 (2) ◽

pp. 747-747

Author(s):

E Editorial

Keyword(s):

Biological Sciences ◽

Bone Marrow ◽

Chromosome Aberrations ◽

Bone Marrow Cells ◽

Mouse Bone Marrow ◽

Genotoxic Effects ◽

Retracted Article ◽

Mouse Bone Marrow Cells ◽

Marrow Cells

This is a notice of retraction of the article: Fumagillin-induced chromosome aberrations in mouse bone-marrow cells, published in the Archives of Biological Sciences in 2010, Vol. 62, Issue 1. The Editor-in-Chief has been informed that this paper plagiarizes an earlier paper: Kulic M, Aleksic N, Stanimirovic Z, Ristic S, Medenica S. Examination of genotoxic effects of fumagillin in vivo. Genetika. 2009;41(3):329-38. The results presented in the article that is being retracted partially overlap with the results in the original article without appropriate justification, permission or crossreferencing. Further, Figs. 1 and 2 in the retracted article also appear in: Stanimirovic Z, Stevanovic J, Kulic M, Stojic V. Frequency of chromosomal aberrations in the evaluation of genotoxic potential of dicyclohexylamine (fumagillin) in vivo. Acta Veterinaria. 2006;56(4):353-66. and in Stanimirovic Z, Stevanovic J, Bajic V, Radovic I. Evaluation of genotoxic effects of fumagillin by cytogenetic tests in vivo. Mutat Res. 2007;628(1):1-10. These figures (in the retracted article) have been subsequently tampered with and therefore present falsified results. After confirmation of this fact, the Editor-in-Chief of the Archives of Biological Sciences has decided to retract the paper immediately. We apologize to the readers of the journal that it took so many years to notice this error and to retract the paper. We request readers of the journal to directly get in touch with the editorial office and the editors of the journal for similar cases in the future, so that they can be handled promptly. Link to the retracted article <a href="http://dx.doi.org/10.2298/ABS1001047S">10.2298/ABS1001047S</a>

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