Maintenance of pancreatic endocrine cells of the neonatal rat: IV. The effect of 3-amino-3-deoxyglucose.

To evaluate the capacity of pancreatic endocrine cells to reassociate in vitro according to the characteristic topographical pattern observed in the islets of Langerhans in situ, we cultured cells dissociated from neonatal rat pancreas within a three-dimensional collagen matrix. Cell monolayers grown on the surface of collagen gels were covered with a second layer of collagen. This induced the monolayers of endocrine cells to reorganize into smooth-contoured, three-dimensional aggregates, in which non-B cells (identified by electron microscopy and immunofluorescence) had a preferential distribution at the periphery, whereas B cells were concentrated in a central position. These results show that cultured pancreatic endocrine cells have the capacity to reassociate into islet-like organoids in vitro, and that collagen matrices may have a permissive effect on the expression of this potential.

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Long-Term Culture of Neonatal Rat Pancreatic Endocrine Cells as Model for Insulin-Secretion and Ion-Channel Studies

Diabetes ◽

10.2337/diab.39.11.1353 ◽

1990 ◽

Vol 39 (11) ◽

pp. 1353-1360 ◽

Cited By ~ 5

Author(s):

J.-L. Schwartz ◽

G. A. R. Mealing ◽

J. F. Whitfield ◽

J. T. Braaten

Keyword(s):

Insulin Secretion ◽

Ion Channel ◽

Endocrine Cells ◽

Neonatal Rat ◽

Long Term Culture ◽

Pancreatic Endocrine Cells

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The distribution of endocrine cell progenitors in the gut of chick embryos

Development ◽

10.1242/dev.82.1.131 ◽

1984 ◽

Vol 82 (1) ◽

pp. 131-145

Author(s):

B. B. Rawdon ◽

Beverley Kramer ◽

Ann Andrew

Keyword(s):

Gastrointestinal Tract ◽

Progenitor Cells ◽

Digestive Tract ◽

Pancreatic Polypeptide ◽

Endocrine Cell ◽

Endocrine Cells ◽

Chick Embryos ◽

Intact Embryo ◽

Pancreatic Endocrine Cells ◽

Gut Endocrine Cells

The aim of this experiment was to find out whether or not, at early stages of development, progenitors of the various types of gut endocrine cells are localized to one or more specific regions of the gastrointestinal tract. Transverse strips of blastoderm two to four somites in length were excised between the levels of somites 5 and 27 in chick embryos at 5- to 24-somite stages and were cultured as chorioallantoic grafts. The distribution of endocrine cells in the grafts revealed confined localization of progenitor cells only in the case of insulinimmunoreactive cells. Theprogenitors of cells with somatostatin-, pancreatic polypeptide-, glucagon-, secretin-, gastrin/CCK-, motilin-, neurotensin- and serotonin-like immunoreactivity were distributed along the length of the presumptive gut at the time of explantation; indeed, in many cases they were more widespread than are their differentiated progeny in normal gut of the same age. This finding indicates that conditions in grafts must differ from those that operate in the intact embryo. Also it may explain the occurrence of ectopic gut or pancreatic endocrine cells in tumours of the digestive tract.

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An immunohistochemical study of pancreatic endocrine cells in SKH-1 hairless mice

European Journal of Histochemistry ◽

10.4081/1684 ◽

2010 ◽

Vol 46 (3) ◽

pp. 229 ◽

Cited By ~ 4

Author(s):

SK Ku ◽

HS Lee ◽

JH Lee

Keyword(s):

Endocrine Cells ◽

Immunohistochemical Study ◽

Hairless Mice ◽

Pancreatic Endocrine Cells

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Effect of long-term culture on the expression of antigens and adhesion molecule in single porcine pancreatic endocrine cells

Xenotransplantation ◽

10.1111/j.1399-3089.2005.00232.x ◽

2005 ◽

Vol 12 (4) ◽

pp. 327-332 ◽

Cited By ~ 5

Author(s):

Kazuya Edamura ◽

Koko Nasu ◽

Yukiko Iwami ◽

Ryohei Nishimura ◽

Hiroyuki Ogawa ◽

...

Keyword(s):

Adhesion Molecule ◽

Endocrine Cells ◽

Long Term Culture ◽

Pancreatic Endocrine Cells

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Epigenetic-Mediated Reprogramming of Pancreatic Endocrine Cells

Antioxidants and Redox Signaling ◽

10.1089/ars.2014.6103 ◽

2015 ◽

Vol 22 (16) ◽

pp. 1483-1495 ◽

Cited By ~ 2

Author(s):

Prabhu Mathiyalagan ◽

Samuel T. Keating ◽

Keith Al-Hasani ◽

Assam El-Osta

Keyword(s):

Endocrine Cells ◽

Pancreatic Endocrine Cells

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REST is a major negative regulator of endocrine differentiation during pancreas organogenesis

10.1101/2021.03.17.435876 ◽

2021 ◽

Author(s):

Meritxell Rovira ◽

Goutham Atla ◽

Miguel Angel Maestro ◽

Vane Grau ◽

Javier García-Hurtado ◽

...

Keyword(s):

Endocrine Cells ◽

Negative Regulator ◽

Β Cell ◽

Knock Out ◽

Endocrine Differentiation ◽

Conditional Allele ◽

Embryonic Pancreas ◽

Pancreatic Endocrine Cells ◽

Knock Out Mice

SUMMARYUnderstanding genomic regulatory mechanisms of pancreas differentiation is relevant to the pathophysiology of diabetes mellitus, and to the development of replacement therapies. Numerous transcription factors promote β cell differentiation, although less is known about negative regulators. Earlier epigenomic studies suggested that the transcriptional repressor REST could be a suppressor of endocrine gene programs in the embryonic pancreas. However, pancreaticRestknock-out mice failed to show increased numbers of endocrine cells, suggesting that REST is not a major regulator of endocrine differentiation. Using a different conditional allele that enables profound REST inactivation, we now observe a marked increase in the formation of pancreatic endocrine cells. REST inhibition also promoted endocrinogenesis in zebrafish and mouse early postnatal ducts, and induced β-cell specific genes in human adult duct-derived organoids. Finally, we define REST genomic programs that suppress pancreatic endocrine differentiation. These results establish a crucial role of REST as a negative regulator of pancreatic endocrine differentiation.

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