GTP cyclohydrolase I from Escherichia coli. Molecular cloning and crystallization

1990 ◽  
pp. 312-315
Keyword(s):  
Escherichia Coli ◽  
Molecular Cloning ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase

Preparation of Isotope-labeled Dihydroneopterin 3'-Triphosphate with High Specific Activity

Pteridines ◽  
1990 ◽  
Vol 2 (3) ◽  
pp. 169-174 ◽  
Author(s):  
Gerd Katzenmeier ◽  
Bruno Schwarzkopf ◽  
Quang Le Van ◽  
Cornelia Schmid ◽  
Adelbert Bacher
Keyword(s):  
Escherichia Coli ◽  
Adenylate Kinase ◽  
Specific Activity ◽  
High Specific Activity ◽  
Starting Material ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase ◽  
Guanylate Kinase ◽  
Phosphoribosyl Transferase ◽  
Phosphoribosylpyrophosphate Synthetase

SummaryIsotope-labeled dihydroneopterin 3'-triphosphate with 3H at positions C-1' and C-2', respectively, has been prepared from isotope-labeled glucose as starting material. Glucose was first converted enzymatically to ribose 5-phosphate. GMP was subsequently obtained by the action of phosphoribosylpyrophosphate synthetase and guanosine phosphoribosyl transferase. It was subsequently phosphorylated to GTP in two steps using adenylate kinase and guanylate kinase. Dihydroneopterin triphosphate was prepared from GTP by the action of recombinant GTP-cyclohydrolase I from Escherichia coli. The method allows the incorporation of 3H and 14C isotope labels into any desired position of dihydroneopterin triphosphate. Rapid purfication procedures for phosphoribosylpyrophosphate synthetase and guanosine phosphoribosyl transferase as well as HPLC assays for their determinations are described.

Crystallization and preliminary crystallographic characterization of GTP cyclohydrolase I from Escherichia coli

1992 ◽  
Vol 226 (4) ◽  
pp. 1279-1281 ◽  
Author(s):  
Cornelia Schmid ◽  
Rudolf Ladenstein ◽  
Hartmut Luecke ◽  
Robert Huber ◽  
Adelbert Bacher
Keyword(s):  
Escherichia Coli ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase

scholarly journals Production of Sepiapterin in Escherichia coli by Coexpression of Cyanobacterial GTP Cyclohydrolase I and Human 6-Pyruvoyltetrahydropterin Synthase

2002 ◽  
Vol 68 (6) ◽  
pp. 3138-3140 ◽  
Author(s):  
Hyun Joo Woo ◽  
Jee Yun Kang ◽  
Yong Kee Choi ◽  
Young Shik Park
Keyword(s):  
Escherichia Coli ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aldose Reductase ◽  
High Performance ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase ◽  
E Coli ◽  
Sepiapterin Reductase ◽  
Pcc 6803

ABSTRACT Synechocystis sp. strain PCC 6803 GTP cyclohydrolase I and human 6-pyruvoyltetrahydropterin synthase were coexpressed in Escherichia coli. The E. coli transformant produced sepiapterin, which was identified by high-performance liquid chromatography and enzymatically converted to dihydrobiopterin by sepiapterin reductase. Aldose reductase, another indispensable enzyme for sepiapterin production, may be endogenous in E. coli.

scholarly journals Location of the gene coding for GTP cyclohydrolase I on the physical map of Escherichia coli.:

1993 ◽  
Vol 175 (5) ◽  
pp. 1553 ◽  
Author(s):  
H Ritz ◽  
G Keller ◽  
G Richter ◽  
G Katzenmeier ◽  
A Bacher
Keyword(s):  
Escherichia Coli ◽  
Physical Map ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase ◽  
Gene Coding

scholarly journals Allosteric characteristics of GTP cyclohydrolase I from Escherichia coli

1992 ◽  
Vol 210 (2) ◽  
pp. 561-568 ◽  
Author(s):  
Gabriele SCHOEDON ◽  
Udo REDWEIK ◽  
Gerhard FRANK ◽  
Richard G. H. COTTON ◽  
Nenad BLAU
Keyword(s):  
Escherichia Coli ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase

Structure and mechanism of GTP cyclohydrolase I of Escherichia coli

1996 ◽  
Vol 24 (1) ◽  
pp. 37S-37S ◽  
Author(s):  
Herbert Nar ◽  
Robert Huber ◽  
Winfried Meining ◽  
Andreas Bracher ◽  
Markus Fischer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase

Studies on GTP Cyclohydrolase I of Escherichia Coli

1993 ◽  
pp. 157-162 ◽  
Author(s):  
Cornelia Schmid ◽  
Winfried Meining ◽  
Sevil Weinkauf ◽  
Luis Bachmann ◽  
Harald Ritz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase

scholarly journals Biochemical Characterization of Oligomerization of Escherichia coli GTP Cyclohydrolase I

BMB Reports ◽  
2002 ◽  
Vol 35 (3) ◽  
pp. 255-261 ◽  
Author(s):  
Soo-Jin Lee ◽  
Chi-Young Ahn ◽  
Eung-Sik Park ◽  
Deog-Su Hwang ◽  
Jeong-Bin Yim
Keyword(s):  
Escherichia Coli ◽  
Biochemical Characterization ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase

scholarly journals Location of the gene coding for GTP cyclohydrolase I on the physical map of Escherichia coli.

1993 ◽  
Vol 175 (5) ◽  
pp. 1553
Author(s):  
H Ritz ◽  
G Keller ◽  
G Richter ◽  
G Katzenmeier ◽  
A Bacher
Keyword(s):  
Escherichia Coli ◽  
Physical Map ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase ◽  
Gene Coding
Sign in / Sign up

Export Citation Format

Share Document