Production of Sepiapterin in Escherichia coli by Coexpression of Cyanobacterial GTP Cyclohydrolase I and Human 6-Pyruvoyltetrahydropterin Synthase
2002 ◽
Vol 68
(6)
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pp. 3138-3140
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Keyword(s):
E Coli
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ABSTRACT Synechocystis sp. strain PCC 6803 GTP cyclohydrolase I and human 6-pyruvoyltetrahydropterin synthase were coexpressed in Escherichia coli. The E. coli transformant produced sepiapterin, which was identified by high-performance liquid chromatography and enzymatically converted to dihydrobiopterin by sepiapterin reductase. Aldose reductase, another indispensable enzyme for sepiapterin production, may be endogenous in E. coli.
2000 ◽
Vol 740
(1)
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pp. 35-42
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1990 ◽
Vol 190
(1)
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pp. 120-128
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pp. 125-131
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pp. 43-51
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2018 ◽
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1985 ◽
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1998 ◽
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pp. 127-135
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