Structure and Function of L-Lactate Dehydrogenases from Thermophilic and Mesophilic Bacteria. I) Isolation and Characterization of Lactate Dehydrogenases from Thermophilic and Mesophilic Bacilli

1979 ◽  
Vol 360 (2) ◽  
pp. 795-808 ◽  
Author(s):  
Hans-Peter SCHÄR ◽  
Herbert ZUBER
Keyword(s):  
Structure And Function ◽  
Mesophilic Bacteria ◽  
Isolation And Characterization ◽  
And Function ◽  
Lactate Dehydrogenases

scholarly journals Conservation of dishevelled structure and function between flies and mice: isolation and characterization of Dvl2

1996 ◽  
Vol 58 (1-2) ◽  
pp. 15-26 ◽  
Author(s):  
J. Klingensmith ◽  
Y. Yang ◽  
J.D. Axelrod ◽  
D.R. Beier ◽  
N. Perrimon ◽  
...  
Keyword(s):  
Structure And Function ◽  
Isolation And Characterization ◽  
And Function

scholarly journals Isolation and Characterization of Nanobodies against a Zinc-Transporting P-Type ATPase

Antibodies ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 39 ◽  
Author(s):  
Elena Longhin ◽  
Christina Grønberg ◽  
Qiaoxia Hu ◽  
Annette Duelli ◽  
Kasper Andersen ◽  
...  
Keyword(s):  
Molecular Level ◽  
Structure And Function ◽  
Structural Studies ◽  
Catalytic Function ◽  
Isolation And Characterization ◽  
Key Players ◽  
Lipid Transporters ◽  
And Function ◽  
P Type

P-type ATPases form a large and ubiquitous superfamily of ion and lipid transporters that use ATP (adenosine triphosphate) to carry out their function. The IB subclass (PIB-ATPases) allows flux of heavy metals and are key players in metal detoxification, critical for human health, crops, and survival of pathogens. Nevertheless, PIB-ATPases remain poorly understood at a molecular level. In this study, nanobodies (Nbs) are selected against the zinc-transporting PIB-ATPase ZntA from Shigella sonnei (SsZntA), aiming at developing tools to assist the characterization of the structure and function of this class of transporters. We identify six different Nbs that bind detergent stabilized SsZntA. We further assess the effect of the Nbs on the catalytic function of SsZntA, and find that five nanobodies associate without affecting the function, while one nanobody significantly reduces the ATPase activity. This study paves the way for more refined mechanistical and structural studies of zinc-transporting PIB-ATPases.

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