scholarly journals Influence of dissolution products of a novel Ca-enriched silicate bioactive glass-ceramic on VEGF release from bone marrow stromal cells

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Preethi Balasubramanian ◽  
Rainer Detsch ◽  
Leticia Esteban-Tejeda ◽  
Alina Grünewald ◽  
José S. Moya ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bioactive Glass ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Healing Process ◽  
Cell Activity ◽  
Marrow Stromal Cells ◽  
Ion Release ◽  
Vegf Release

AbstractThis study evaluated the influence of ionic dissolution products of a novel Ca-enriched silicate bioactive glass compared to commercial available hydroxyapaptite samples (Endobonr) on cell activity and vascular endothelial growth factor (VEGF) release in vitro. Bone marrow stromal cells (ST-2) were cultivated with the supernatant of granules of different sizes and at different concentrations (0-1 wt/vol % of granules) for 48 h. In addition to in vitro studies, Ca-ion release from all as cell morphology observation revealed no cytotoxic effect of the released products from all tested materials. It was found that supernatants from granules in concentrations of 1 wt/vol %enhanced the VEGF release from ST2 cells, which is important as a marker of the vascularisation ability of the glass during the bone healing process.

scholarly journals A Method for Measurement of Drug Sensitivity of Myeloma Cells Co-Cultured with Bone Marrow Stromal Cells

2013 ◽  
Vol 18 (6) ◽  
pp. 637-646 ◽  
Author(s):  
Kristine Misund ◽  
Katarzyna A. Baranowska ◽  
Toril Holien ◽  
Christoph Rampa ◽  
Dionne C. G. Klein ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Survival ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Large Scale ◽  
Stromal Cell ◽  
Marrow Stromal Cells ◽  
Cell Nuclei ◽  
Myeloma Cells

The tumor microenvironment can profoundly affect tumor cell survival as well as alter antitumor drug activity. However, conventional anticancer drug screening typically is performed in the absence of stromal cells. Here, we analyzed survival of myeloma cells co-cultured with bone marrow stromal cells (BMSC) using an automated fluorescence microscope platform, ScanR. By staining the cell nuclei with DRAQ5, we could distinguish between BMSC and myeloma cells, based on their staining intensity and nuclear shape. Using the apoptotic marker YO-PRO-1, the effects of drug treatment on the viability of the myeloma cells in the presence of stromal cells could be measured. The method does not require cell staining before incubation with drugs, and less than 5000 cells are required per condition. The method can be used for large-scale screening of anticancer drugs on primary myeloma cells. This study shows the importance of stromal cell support for primary myeloma cell survival in vitro, as half of the cell samples had a marked increase in their viability when cultured in the presence of BMSC. Stromal cell–induced protection against common myeloma drugs is also observed with this method.

In vitro differentiation of human bone marrow stromal cells into neural precursor cells using small molecules

2021 ◽  
Vol 363 ◽  
pp. 109340
Author(s):  
Abeer Sallam ◽  
Thangirala Sudha ◽  
Noureldien H.E. Darwish ◽  
Samar Eghotny ◽  
Abeer E-Dief ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Small Molecules ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Precursor Cells ◽  
Marrow Stromal Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
In Vitro Differentiation

scholarly journals Combination of hTERT and bmi-1, E6, or E7 Induces Prolongation of the Life Span of Bone Marrow Stromal Cells from an Elderly Donor without Affecting Their Neurogenic Potential

2005 ◽  
Vol 25 (12) ◽  
pp. 5183-5195 ◽  
Author(s):  
Taisuke Mori ◽  
Tohru Kiyono ◽  
Hideaki Imabayashi ◽  
Yukiji Takeda ◽  
Kohei Tsuchiya ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Life Span ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Murine Bone Marrow ◽  
Differentiated Cells ◽  
E6 And E7 ◽  
Old Donor

ABSTRACT Murine bone marrow stromal cells differentiate not only into mesodermal derivatives, such as osteocytes, chondrocytes, adipocytes, skeletal myocytes, and cardiomyocytes, but also into neuroectodermal cells in vitro. Human bone marrow stromal cells are easy to isolate but difficult to study because of their limited life span. To overcome this problem, we attempted to prolong the life span of bone marrow stromal cells and investigated whether bone marrow stromal cells modified with bmi-1, hTERT, E6, and E7 retained their differentiated capability, or multipotency. In this study, we demonstrated that the life span of bone marrow stromal cells derived from a 91-year-old donor could be extended and that the stromal cells with an extended life span differentiated into neuronal cells in vitro. We examined the neuronally differentiated cells morphologically, physiologically, and biologically and compared the gene profiles of undifferentiated and differentiated cells. The neuronally differentiated cells exhibited characteristics similar to those of midbrain neuronal progenitors. Thus, the results of this study support the possible use of autologous-cell graft systems to treat central nervous system diseases in geriatric patients.

scholarly journals Effect of Fibrin Formulation on Initial Strength of Tendon Repair and Migration of Bone Marrow Stromal Cells in Vitro

2015 ◽  
Vol 97 (21) ◽  
pp. 1792-1798 ◽  
Author(s):  
Kosuke Uehara ◽  
Chunfeng Zhao ◽  
Anne Gingery ◽  
Andrew R. Thoreson ◽  
Kai-Nan An ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Tendon Repair ◽  
Marrow Stromal Cells ◽  
Initial Strength ◽  
And Migration
Sign in / Sign up

Export Citation Format

Share Document