C-heterochromatin and NORs distribution in karyotypes of three vespertilionid bat species from Turkey

Biologia ◽  
2015 ◽  
Vol 70 (3) ◽  
Author(s):  
Atilla Arslan ◽  
Jan Zima ◽  
Irfan Albayrak ◽  
Tarkan Yorulmaz ◽  
Emine Arslan
Keyword(s):  
Moderate Amount ◽  
C Banding ◽  
Banding Analysis ◽  
Autosomal Pair ◽  
Chromosomal Banding ◽  
Eptesicus Serotinus

AbstractThe chromosomal banding analysis of the karyotypes of Turkish populations of Eptesicus serotinus, Nyctalus lasiopterus and Barbastellus barbastellus was performed with the use of C-banding and Ag-NOR staining. The results obtained in E. serotinus and N. lasiopterus were congruent with previous data reported from other regions. The karyotype of E. serotinus (2n = 50, NF = 52) contained a moderate amount of centromeric C-heterochromatin and a single NOR was localized in an acrocentric autosomal pairs. The karyotype of N. lasiopterus (2n = 42, NF = 54) contained a higher amount of centromeric C-heterochromatin and the NORs were localized in two autosomal pairs. The karyotype of B. barbastellus was standard in its general characteristics (2n = 32, NF = 54, low amount of C-heterochromatin) but the NOR was localized in only one acrocentric autosomal pair. In studies from other regions, the NORs were recognized in all five acrocentric autosomal pairs of the complement of B. barbastellus.

scholarly journals Karyotypic diversity among three species of the genus Astyanax (Characiformes: Characidae)

2016 ◽  
Vol 76 (2) ◽  
pp. 360-366 ◽  
Author(s):  
P. B. Nishiyama ◽  
M. M. R. Vieira ◽  
F. E. Porto ◽  
L. A. Borin ◽  
A. L. B. Portela-Castro ◽  
...  
Keyword(s):  
Diploid Number ◽  
Fish Fauna ◽  
Distinct Pattern ◽  
Supernumerary Chromosome ◽  
C Banding ◽  
Incertae Sedis ◽  
Banding Analysis ◽  
Karyotypic Diversity ◽  
Karyotype Formula

Abstract The group Incertae sedis within the Characidae family currently includes 88 genera, previously included in the subfamily Tetragonopterinae. Among them is the genus Astyanax comprising a group of species with similar morphology and widely distributed in the Neotropics. Thus, the present study aimed to analyze the karyotype diversity in Astyanax species from different watersheds by conventional Giemsa staining, C-banding and fluorescence in situ hybridization (FISH rDNA 18S) probe.specimens of Astyanax aff. paranae belonging to the “scabripinnis complex”, Astyanax asunsionensis and Astyanax aff. bimaculatus were analyzed”. Two sympatric karyomorphs were observed in Astyanax.aff paranae, one of them having2n=48andthe other one with 2n=50 chromosomes. Other population of this same species also presented 2n=50 chromosomes, but differing in the karyotype formula and with macro supernumerary chromosome found in 100% of the cells in about 80%of females analyzed. Two population of A. asuncionensis and one population of Astyanax. aff. bimaculatus, also showed a diploid number of 50 chromosomes, but also differing in their karyotype formulas. Therefore, A. asuncionensis was also characterized by intraspecific chromosome diversity. The C-banding analysis was able to demonstrate a distinctable to demonstrate a distinct pattern of heterochromatin differing A. asuncionensis from Astyanax aff. paranae and Astyanax aff. bimaculatus. The supernumerary chromosome of Astyanax aff. paranae proved completely heterochromatic. Only Astyanax.aff. bimaculatus multiple showed multiple sites of nucleolar organizing regions. The other species were characterized by having a simple system of NOR. These data contributes to the know ledge of the existing biodiversity in our fish fauna, here highlighted by the inter- and intraspecific chromosomal diversity in the genus Astyanax.

Mapping of the leaf rust resistance gene Lr3 on chromosome 6B of Sinvalocho MA wheat

Genome ◽  
1998 ◽  
Vol 41 (5) ◽  
pp. 686-690 ◽  
Author(s):  
F Sacco ◽  
E Y Suárez ◽  
T Naranjo
Keyword(s):  
Leaf Rust ◽  
Rust Resistance ◽  
Centromeric Region ◽  
Leaf Rust Resistance ◽  
Rflp Markers ◽  
Leaf Rust Resistance Gene ◽  
C Banding ◽  
Key Words Wheat ◽  
Naturally Occurring ◽  
Banding Analysis

The Lr3 gene for resistance to race 66 of Puccinia recondita present in hexaploid wheat cv. Sinvalocho MA was mapped on chromosome 6B, using intervarietal polymorphic RFLP loci and the Amp-B1 isozyme gene as a centromere marker. The RFLP markers were located mainly in two subregions of chromosome 6BL. Six RFLP loci clustered in the centromeric region and one other, Xmwg798, cosegregated with the Lr3 gene. C-banding analysis of the leaf rust resistant standard 'Sinvalocho MA' line and three naturally occurring susceptible lines of 'Sinvalocho MA' revealed a terminal deletion on 6BL that covered 20% of its length in one susceptible line. Because Xmwg798 was missing in this line, both Xmwg798 and Lr3 were allocated to the deleted segment. Distorted segregations were observed for the proximal markers, suggesting a selection against gametes carrying the centromeric region of 'Sinvalocho MA'.Key words: wheat, RFLP, Lr3 gene, chromosome 6B, C-banding.

Location of a Triticum speltoides chromosome segment conferring resistance to leaf rust in Triticum aestivum

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 892-897 ◽  
Author(s):  
J. Dvořák ◽  
D. R. Knott
Keyword(s):  
Triticum Aestivum ◽  
Leaf Rust ◽  
Wheat Chromosome ◽  
Leaf Rust Resistance ◽  
Leaf Rust Resistance Gene ◽  
Backcross Generation ◽  
Transfer Lines ◽  
C Banding ◽  
Banding Analysis ◽  
Speltoides Chromosome

A leaf rust resistant line, 2-9-2, was selected in the fourth backcross generation to Triticum aestivum of an interspecific hybrid, T. aestivum × Triticum speltoides. The resistance segregated independently of T. speltoides leaf rust resistance gene Lr28, previously shown to be incorporated into wheat chromosome 1B in two other transfer lines. Monosomic and telosomic analyses showed that the gene in line 2-9-2, Lr36, was incorporated into the short arm of chromosome 6B. C-banding analysis showed that the homoeologous crossing-over occurred distally to an interstitial C-band in the satellite and linkage analysis showed Lr36 to be tightly linked to the telomeric C-band.Key words: C-banding, physical mapping, linkage, wheat, chromosome 6B, introgression.

C-Banded Karyotype and Cytotaxonomy of Mertensiella caucasica (WAGA, 1876) (Caudata: Salamandridae)

1982 ◽  
Vol 3 (4) ◽  
pp. 303-307 ◽  
Author(s):  
Matilde Ragghianti ◽  
Stefania Bucci-Innocenti ◽  
Giorgio Mancino
Keyword(s):  
Chromosome Number ◽  
Banding Pattern ◽  
Related Form ◽  
C Banding ◽  
Structural Character ◽  
Chromosomal Banding

AbstractChromosome number, karyotype and chromosomal banding pattern of Mertensiella caucasica were studied in differentially stained mitotic preparations. The main feature of the C-banded appearance concerns the centromere regions, which are heavily stained by the Giemsa and are outlined by proximal C-bands. This morpho-structural character seems to be peculiar of the genome of M. caucasica, when compared with the C-banding pattern shown by the chromosomes of Salamandra, considered the most closely related form. Therefore, a preliminary cytotaxonomic survey seems to support the opinion that the Caucasian salamander can be referred to a well defined genus Mertensiella rather than a subgenus of Salamandra.

scholarly journals Giemsa C-banding Analysis of Sternbergi lutea (L.) Ker-Gawl. ex Sprengel and S. sicula Tineo ex Guss. from Turkey.

CYTOLOGIA ◽  
1997 ◽  
Vol 62 (1) ◽  
pp. 1-6 ◽  
Author(s):  
D. Yüzbasioglu ◽  
F. Ünal ◽  
H. Duman
Keyword(s):  
C Banding ◽  
Banding Analysis

scholarly journals Comparative C-banding and Fluorescent-banding Analysis of Seven Species of Drosera (Droseraceae).

CYTOLOGIA ◽  
1996 ◽  
Vol 61 (4) ◽  
pp. 383-394 ◽  
Author(s):  
Shamimul Alam Sheikh ◽  
Katsuhiko Kondo
Keyword(s):  
Fluorescent Banding ◽  
C Banding ◽  
Banding Analysis

Chromosome structure of Triticum timopheevii relative to T. turgidum

Genome ◽  
2000 ◽  
Vol 43 (6) ◽  
pp. 923-930 ◽  
Author(s):  
S Rodríguez ◽  
E Perera ◽  
B Maestra ◽  
M Díez ◽  
T Naranjo
Keyword(s):  
Chromosome Structure ◽  
Triticum Turgidum ◽  
Chromosomal Rearrangements ◽  
In Situ Hybridisation ◽  
Triticum Timopheevii ◽  
C Banding ◽  
Genomic In Situ Hybridisation ◽  
Banding Analysis ◽  
Wild Accessions ◽  
Species Specific

The chromosome structure of four different wild populations and a cultivated line of Triticum timopheevii (2n = 28, AtAtGG) relative to Triticum turgidum (2n = 28, AABB) was studied, using genomic in situ hybridisation (GISH) and C-banding analysis of meiotic configurations in interspecific hybrids. Two wild accessions and the cultivated line showed the standard C-banding karyotype. The other two accessions are homozygous for translocation 5At/3G and translocations 1G/2G and 5G/6G. GISH analysis revealed that all the T. timopheevii accessions carry intergenome translocations 6At/1G and 1G/4G and identified the position of the breakpoint in translocation 5At/3G. C-banding analysis of pairing at metaphase I in the hybrids with T. turgidum provides evidence that four species-specific translocations (6AtS/1GS, 1GS/4GS, 4GS/4AtL, and 4AtL/3AtL) exist in T. timopheevii, and that T. timopheevii and T. turgidum differ in the pericentric inversion of chromosome 4A. Bridge plus acentric fragment configurations involving 4AL and 4AtL were identified in cells at anaphase I. This result suggests that the paracentric inversion of 4AL from T. turgidum does not exist in T. timopheevii. Both tetraploid species have undergone independent and distinct evolutionary chromosomal rearrangements. The position, intercalary or subdistal, of the breakpoints in species-specific translocations and inversions contrasts with the position, at or close to the centromere, of intraspecific translocations. Different mechanisms for intraspecific and species-specific chromosome rearrangements are suggested.Key words: Triticum timopheevii, chromosome pairing, translocation, evolution, C-banding, GISH.

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