Comparative C-banding and Fluorescent-banding Analysis of Seven Species of Drosera (Droseraceae).

Abstract The group Incertae sedis within the Characidae family currently includes 88 genera, previously included in the subfamily Tetragonopterinae. Among them is the genus Astyanax comprising a group of species with similar morphology and widely distributed in the Neotropics. Thus, the present study aimed to analyze the karyotype diversity in Astyanax species from different watersheds by conventional Giemsa staining, C-banding and fluorescence in situ hybridization (FISH rDNA 18S) probe.specimens of Astyanax aff. paranae belonging to the “scabripinnis complex”, Astyanax asunsionensis and Astyanax aff. bimaculatus were analyzed”. Two sympatric karyomorphs were observed in Astyanax.aff paranae, one of them having2n=48andthe other one with 2n=50 chromosomes. Other population of this same species also presented 2n=50 chromosomes, but differing in the karyotype formula and with macro supernumerary chromosome found in 100% of the cells in about 80%of females analyzed. Two population of A. asuncionensis and one population of Astyanax. aff. bimaculatus, also showed a diploid number of 50 chromosomes, but also differing in their karyotype formulas. Therefore, A. asuncionensis was also characterized by intraspecific chromosome diversity. The C-banding analysis was able to demonstrate a distinctable to demonstrate a distinct pattern of heterochromatin differing A. asuncionensis from Astyanax aff. paranae and Astyanax aff. bimaculatus. The supernumerary chromosome of Astyanax aff. paranae proved completely heterochromatic. Only Astyanax.aff. bimaculatus multiple showed multiple sites of nucleolar organizing regions. The other species were characterized by having a simple system of NOR. These data contributes to the know ledge of the existing biodiversity in our fish fauna, here highlighted by the inter- and intraspecific chromosomal diversity in the genus Astyanax.

Download Full-text

C-heterochromatin and NORs distribution in karyotypes of three vespertilionid bat species from Turkey

Biologia ◽

10.1515/biolog-2015-0044 ◽

2015 ◽

Vol 70 (3) ◽

Author(s):

Atilla Arslan ◽

Jan Zima ◽

Irfan Albayrak ◽

Tarkan Yorulmaz ◽

Emine Arslan

Keyword(s):

Moderate Amount ◽

C Banding ◽

Banding Analysis ◽

Autosomal Pair ◽

Chromosomal Banding ◽

Eptesicus Serotinus

AbstractThe chromosomal banding analysis of the karyotypes of Turkish populations of Eptesicus serotinus, Nyctalus lasiopterus and Barbastellus barbastellus was performed with the use of C-banding and Ag-NOR staining. The results obtained in E. serotinus and N. lasiopterus were congruent with previous data reported from other regions. The karyotype of E. serotinus (2n = 50, NF = 52) contained a moderate amount of centromeric C-heterochromatin and a single NOR was localized in an acrocentric autosomal pairs. The karyotype of N. lasiopterus (2n = 42, NF = 54) contained a higher amount of centromeric C-heterochromatin and the NORs were localized in two autosomal pairs. The karyotype of B. barbastellus was standard in its general characteristics (2n = 32, NF = 54, low amount of C-heterochromatin) but the NOR was localized in only one acrocentric autosomal pair. In studies from other regions, the NORs were recognized in all five acrocentric autosomal pairs of the complement of B. barbastellus.

Download Full-text

Mapping of the leaf rust resistance gene Lr3 on chromosome 6B of Sinvalocho MA wheat

Genome ◽

10.1139/g98-067 ◽

1998 ◽

Vol 41 (5) ◽

pp. 686-690 ◽

Cited By ~ 24

Author(s):

F Sacco ◽

E Y Suárez ◽

T Naranjo

Keyword(s):

Leaf Rust ◽

Rust Resistance ◽

Centromeric Region ◽

Leaf Rust Resistance ◽

Rflp Markers ◽

Leaf Rust Resistance Gene ◽

C Banding ◽

Key Words Wheat ◽

Naturally Occurring ◽

Banding Analysis

The Lr3 gene for resistance to race 66 of Puccinia recondita present in hexaploid wheat cv. Sinvalocho MA was mapped on chromosome 6B, using intervarietal polymorphic RFLP loci and the Amp-B1 isozyme gene as a centromere marker. The RFLP markers were located mainly in two subregions of chromosome 6BL. Six RFLP loci clustered in the centromeric region and one other, Xmwg798, cosegregated with the Lr3 gene. C-banding analysis of the leaf rust resistant standard 'Sinvalocho MA' line and three naturally occurring susceptible lines of 'Sinvalocho MA' revealed a terminal deletion on 6BL that covered 20% of its length in one susceptible line. Because Xmwg798 was missing in this line, both Xmwg798 and Lr3 were allocated to the deleted segment. Distorted segregations were observed for the proximal markers, suggesting a selection against gametes carrying the centromeric region of 'Sinvalocho MA'.Key words: wheat, RFLP, Lr3 gene, chromosome 6B, C-banding.

Download Full-text

Location of a Triticum speltoides chromosome segment conferring resistance to leaf rust in Triticum aestivum

Genome ◽

10.1139/g90-134 ◽

1990 ◽

Vol 33 (6) ◽

pp. 892-897 ◽

Cited By ~ 30

Author(s):

J. Dvořák ◽

D. R. Knott

Keyword(s):

Triticum Aestivum ◽

Leaf Rust ◽

Wheat Chromosome ◽

Leaf Rust Resistance ◽

Leaf Rust Resistance Gene ◽

Backcross Generation ◽

Transfer Lines ◽

C Banding ◽

Banding Analysis ◽

Speltoides Chromosome

A leaf rust resistant line, 2-9-2, was selected in the fourth backcross generation to Triticum aestivum of an interspecific hybrid, T. aestivum × Triticum speltoides. The resistance segregated independently of T. speltoides leaf rust resistance gene Lr28, previously shown to be incorporated into wheat chromosome 1B in two other transfer lines. Monosomic and telosomic analyses showed that the gene in line 2-9-2, Lr36, was incorporated into the short arm of chromosome 6B. C-banding analysis showed that the homoeologous crossing-over occurred distally to an interstitial C-band in the satellite and linkage analysis showed Lr36 to be tightly linked to the telomeric C-band.Key words: C-banding, physical mapping, linkage, wheat, chromosome 6B, introgression.

Download Full-text

C-banding analysis on wild Emmer (Triticum dicoccoides K�rn) strains with and without spontaneous reciprocal translocations

Theoretical and Applied Genetics ◽

10.1007/s001220050111 ◽

1996 ◽

Vol 92 (2) ◽

pp. 173-178 ◽

Cited By ~ 2

Author(s):

S. Taketa ◽

T. Kawahara

Keyword(s):

Triticum Dicoccoides ◽

Wild Emmer ◽

Reciprocal Translocations ◽

C Banding ◽

Banding Analysis

Download Full-text

Orcein and Fluorescent Banding Analysis of Two Floral Types of Catharanthus Roseus L.

Dhaka University Journal of Biological Sciences ◽

10.3329/dujbs.v29i2.48765 ◽

2020 ◽

Vol 29 (2) ◽

pp. 219-227

Author(s):

Tanusree Datta ◽

Meghla Saha Pinky ◽

Chandan Kumar Dash ◽

Kazi Nahida Begum

Keyword(s):

Catharanthus Roseus ◽

Differential Staining ◽

Future Research ◽

Interphase Nuclei ◽

Continuous Type ◽

Fluorescent Banding ◽

Banding Analysis ◽

Taxonomical Status ◽

Gradient Type ◽

Combined Data

Two floral types of Catharanthus roseus L. viz. pink and white were studied through differential staining with orcein, CMA and DAPI for cytogenetical characterization and to assist towards updating their taxonomical status and evaluating chromosomal diversity between them. "Simple Chromocenter Type" of interphase nuclei was observed with some darkly stained small heterochromatic regions throughout the nuclei. Most of the prophase chromosomes of Catharanthus roseus (pink and white) were "Continuous Type" and a few were "Gradient Type". Although these two floral types possessed 16 metacentric chromosomes in somatic cells, they showed variation in fluorescent banding pattern considering the modification of GC- and AT-rich repetitive segments. Taking into account all the parameters of both the floral types of C. roseus showed strict symmetric karyotype as well as primitive nature. Therefore, the combined data of differential staining provide information to make comments on their chromosomal status with cytogenetical characterization and also create a baseline for future research. Dhaka Univ. J. Biol. Sci. 29(2): 219-227, 2020 (July)

Download Full-text

Giemsa C-banding Analysis of Sternbergi lutea (L.) Ker-Gawl. ex Sprengel and S. sicula Tineo ex Guss. from Turkey.

CYTOLOGIA ◽

10.1508/cytologia.62.1 ◽

1997 ◽

Vol 62 (1) ◽

pp. 1-6 ◽

Cited By ~ 4

Author(s):

D. Yüzbasioglu ◽

F. Ünal ◽

H. Duman

Keyword(s):

C Banding ◽

Banding Analysis

Download Full-text

Chromosome structure of Triticum timopheevii relative to T. turgidum

Genome ◽

10.1139/g00-062 ◽

2000 ◽

Vol 43 (6) ◽

pp. 923-930 ◽

Cited By ~ 11

Author(s):

S Rodríguez ◽

E Perera ◽

B Maestra ◽

M Díez ◽

T Naranjo

Keyword(s):

Chromosome Structure ◽

Triticum Turgidum ◽

Chromosomal Rearrangements ◽

In Situ Hybridisation ◽

Triticum Timopheevii ◽

C Banding ◽

Genomic In Situ Hybridisation ◽

Banding Analysis ◽

Wild Accessions ◽

Species Specific

The chromosome structure of four different wild populations and a cultivated line of Triticum timopheevii (2n = 28, AtAtGG) relative to Triticum turgidum (2n = 28, AABB) was studied, using genomic in situ hybridisation (GISH) and C-banding analysis of meiotic configurations in interspecific hybrids. Two wild accessions and the cultivated line showed the standard C-banding karyotype. The other two accessions are homozygous for translocation 5At/3G and translocations 1G/2G and 5G/6G. GISH analysis revealed that all the T. timopheevii accessions carry intergenome translocations 6At/1G and 1G/4G and identified the position of the breakpoint in translocation 5At/3G. C-banding analysis of pairing at metaphase I in the hybrids with T. turgidum provides evidence that four species-specific translocations (6AtS/1GS, 1GS/4GS, 4GS/4AtL, and 4AtL/3AtL) exist in T. timopheevii, and that T. timopheevii and T. turgidum differ in the pericentric inversion of chromosome 4A. Bridge plus acentric fragment configurations involving 4AL and 4AtL were identified in cells at anaphase I. This result suggests that the paracentric inversion of 4AL from T. turgidum does not exist in T. timopheevii. Both tetraploid species have undergone independent and distinct evolutionary chromosomal rearrangements. The position, intercalary or subdistal, of the breakpoints in species-specific translocations and inversions contrasts with the position, at or close to the centromere, of intraspecific translocations. Different mechanisms for intraspecific and species-specific chromosome rearrangements are suggested.Key words: Triticum timopheevii, chromosome pairing, translocation, evolution, C-banding, GISH.

Download Full-text