The C-banding analysis of the diploid male and female honeybee (Apis mellifera).

A dioecious grass Sohnsia filifolia (Fourn.) Airy Shaw (Syn. Calamochloa filifolia Fourn.) from Mexico has been found to have 2n = 20 chromosomes in both male and female plants. The staminate plants have one chromosome much longer than the other chromosomes of the complement. One pistillate plant was found to have 30 chromosomes, among which the largest chromosome is quite similar to the largest component of the diploid male plant. The longest chromosome has been designated as the Y chromosome. An XY-mechanism of the Drosophilia type has been suggested for the sex determination system in this species. One small supernumerary chromosome was observed in the microsporocytes of some male plants, but was absent in roots.

Download Full-text

Karyotypic diversity among three species of the genus Astyanax (Characiformes: Characidae)

Brazilian Journal of Biology ◽

10.1590/1519-6984.15414 ◽

2016 ◽

Vol 76 (2) ◽

pp. 360-366 ◽

Cited By ~ 2

Author(s):

P. B. Nishiyama ◽

M. M. R. Vieira ◽

F. E. Porto ◽

L. A. Borin ◽

A. L. B. Portela-Castro ◽

...

Keyword(s):

Diploid Number ◽

Fish Fauna ◽

Distinct Pattern ◽

Supernumerary Chromosome ◽

C Banding ◽

Incertae Sedis ◽

Banding Analysis ◽

Karyotypic Diversity ◽

Karyotype Formula

Abstract The group Incertae sedis within the Characidae family currently includes 88 genera, previously included in the subfamily Tetragonopterinae. Among them is the genus Astyanax comprising a group of species with similar morphology and widely distributed in the Neotropics. Thus, the present study aimed to analyze the karyotype diversity in Astyanax species from different watersheds by conventional Giemsa staining, C-banding and fluorescence in situ hybridization (FISH rDNA 18S) probe.specimens of Astyanax aff. paranae belonging to the “scabripinnis complex”, Astyanax asunsionensis and Astyanax aff. bimaculatus were analyzed”. Two sympatric karyomorphs were observed in Astyanax.aff paranae, one of them having2n=48andthe other one with 2n=50 chromosomes. Other population of this same species also presented 2n=50 chromosomes, but differing in the karyotype formula and with macro supernumerary chromosome found in 100% of the cells in about 80%of females analyzed. Two population of A. asuncionensis and one population of Astyanax. aff. bimaculatus, also showed a diploid number of 50 chromosomes, but also differing in their karyotype formulas. Therefore, A. asuncionensis was also characterized by intraspecific chromosome diversity. The C-banding analysis was able to demonstrate a distinctable to demonstrate a distinct pattern of heterochromatin differing A. asuncionensis from Astyanax aff. paranae and Astyanax aff. bimaculatus. The supernumerary chromosome of Astyanax aff. paranae proved completely heterochromatic. Only Astyanax.aff. bimaculatus multiple showed multiple sites of nucleolar organizing regions. The other species were characterized by having a simple system of NOR. These data contributes to the know ledge of the existing biodiversity in our fish fauna, here highlighted by the inter- and intraspecific chromosomal diversity in the genus Astyanax.

Download Full-text

C-heterochromatin and NORs distribution in karyotypes of three vespertilionid bat species from Turkey

Biologia ◽

10.1515/biolog-2015-0044 ◽

2015 ◽

Vol 70 (3) ◽

Author(s):

Atilla Arslan ◽

Jan Zima ◽

Irfan Albayrak ◽

Tarkan Yorulmaz ◽

Emine Arslan

Keyword(s):

Moderate Amount ◽

C Banding ◽

Banding Analysis ◽

Autosomal Pair ◽

Chromosomal Banding ◽

Eptesicus Serotinus

AbstractThe chromosomal banding analysis of the karyotypes of Turkish populations of Eptesicus serotinus, Nyctalus lasiopterus and Barbastellus barbastellus was performed with the use of C-banding and Ag-NOR staining. The results obtained in E. serotinus and N. lasiopterus were congruent with previous data reported from other regions. The karyotype of E. serotinus (2n = 50, NF = 52) contained a moderate amount of centromeric C-heterochromatin and a single NOR was localized in an acrocentric autosomal pairs. The karyotype of N. lasiopterus (2n = 42, NF = 54) contained a higher amount of centromeric C-heterochromatin and the NORs were localized in two autosomal pairs. The karyotype of B. barbastellus was standard in its general characteristics (2n = 32, NF = 54, low amount of C-heterochromatin) but the NOR was localized in only one acrocentric autosomal pair. In studies from other regions, the NORs were recognized in all five acrocentric autosomal pairs of the complement of B. barbastellus.

Download Full-text

Mapping of the leaf rust resistance gene Lr3 on chromosome 6B of Sinvalocho MA wheat

Genome ◽

10.1139/g98-067 ◽

1998 ◽

Vol 41 (5) ◽

pp. 686-690 ◽

Cited By ~ 24

Author(s):

F Sacco ◽

E Y Suárez ◽

T Naranjo

Keyword(s):

Leaf Rust ◽

Rust Resistance ◽

Centromeric Region ◽

Leaf Rust Resistance ◽

Rflp Markers ◽

Leaf Rust Resistance Gene ◽

C Banding ◽

Key Words Wheat ◽

Naturally Occurring ◽

Banding Analysis

The Lr3 gene for resistance to race 66 of Puccinia recondita present in hexaploid wheat cv. Sinvalocho MA was mapped on chromosome 6B, using intervarietal polymorphic RFLP loci and the Amp-B1 isozyme gene as a centromere marker. The RFLP markers were located mainly in two subregions of chromosome 6BL. Six RFLP loci clustered in the centromeric region and one other, Xmwg798, cosegregated with the Lr3 gene. C-banding analysis of the leaf rust resistant standard 'Sinvalocho MA' line and three naturally occurring susceptible lines of 'Sinvalocho MA' revealed a terminal deletion on 6BL that covered 20% of its length in one susceptible line. Because Xmwg798 was missing in this line, both Xmwg798 and Lr3 were allocated to the deleted segment. Distorted segregations were observed for the proximal markers, suggesting a selection against gametes carrying the centromeric region of 'Sinvalocho MA'.Key words: wheat, RFLP, Lr3 gene, chromosome 6B, C-banding.

Download Full-text

On sex determination in the Turkish desert woodlouse Hemilepistus elongatus (Crustacea, Isopoda, Oniscidea): searching for sex chromosomes and for sex-specific differences in simple DNA repeats

Genome ◽

10.1139/g96-103 ◽

1996 ◽

Vol 39 (4) ◽

pp. 818-821 ◽

Cited By ~ 3

Author(s):

G. Röder ◽

K. E. Linsenmair ◽

I. Nanda ◽

M. Schmid

Keyword(s):

Chromosome Number ◽

Sex Determination ◽

Sex Chromosomes ◽

Genomic Dna ◽

Constitutive Heterochromatin ◽

Dna Repeats ◽

Male And Female ◽

General Morphology ◽

C Banding ◽

Heteromorphic Sex Chromosomes

The karyotype of male and female Hemilepistus elongatus was investigated by means of C-banding. The diploid chromosome number in both sexes is 2n = 50. By scrutinizing general morphology and localization of the constitutive heterochromatin, no heteromorphic sex chromosomes were found. All chromosome pairs in males are well paired during diakinesis. Hybridization of genomic DNA with (GACA)4 and (GATA)4 oligonucleotides revealed no sex-specific patterns. Key words : karyotype, C-banding, sex determination, simple DNA-repeats, Isopoda.

Download Full-text

Location of a Triticum speltoides chromosome segment conferring resistance to leaf rust in Triticum aestivum

Genome ◽

10.1139/g90-134 ◽

1990 ◽

Vol 33 (6) ◽

pp. 892-897 ◽

Cited By ~ 30

Author(s):

J. Dvořák ◽

D. R. Knott

Keyword(s):

Triticum Aestivum ◽

Leaf Rust ◽

Wheat Chromosome ◽

Leaf Rust Resistance ◽

Leaf Rust Resistance Gene ◽

Backcross Generation ◽

Transfer Lines ◽

C Banding ◽

Banding Analysis ◽

Speltoides Chromosome

A leaf rust resistant line, 2-9-2, was selected in the fourth backcross generation to Triticum aestivum of an interspecific hybrid, T. aestivum × Triticum speltoides. The resistance segregated independently of T. speltoides leaf rust resistance gene Lr28, previously shown to be incorporated into wheat chromosome 1B in two other transfer lines. Monosomic and telosomic analyses showed that the gene in line 2-9-2, Lr36, was incorporated into the short arm of chromosome 6B. C-banding analysis showed that the homoeologous crossing-over occurred distally to an interstitial C-band in the satellite and linkage analysis showed Lr36 to be tightly linked to the telomeric C-band.Key words: C-banding, physical mapping, linkage, wheat, chromosome 6B, introgression.

Download Full-text

Flagellar sensilla in male and female European beewolves, Philanthus triangulum F. (Hymenoptera: Sphecidae)

Entomologica Fennica ◽

10.33338/ef.84192 ◽

2003 ◽

Vol 14 (4) ◽

Cited By ~ 3

Author(s):

Gudrun Herzner ◽

Thomas Schmitt ◽

K. Eduard Linsenmair ◽

Erhard Strohm

Keyword(s):

Apis Mellifera ◽

Functional Significance ◽

Antennal Sensilla ◽

Male And Female ◽

Philanthus Triangulum ◽

Sensilla Basiconica ◽

Different Types ◽

The Difference

We investigated the morphology of the antennal sensilla of a sphecid wasp, the European beewolf Philanthus triangulum, to provide an inventory for the species and to compare the sensillar equipment between the sexes. The density of sensilla increased from the base to the tip of the antennae. We distinguished nine different types of sensilla. One type has not yet been described in Hymenoptera. The large sensilla basiconica occurred only on the antennae of female beewolves. We discuss the functional significance of the difference between the sexes and compare our results with data from other sphecids and the honeybee Apis mellifera.

Download Full-text