Impact of specimen mixing methods on presepsin point-of-care test results using whole blood

2016 ◽  
Vol 54 (5) ◽  
Author(s):  
Ji Yeon Ham ◽  
Kyung Eun Song
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Test Results ◽  
Point Of Care Test

scholarly journals Fecal viral DNA shedding following clinical panleukopenia virus infection in shelter kittens: a prospective, observational study

2021 ◽  
pp. 1098612X2110230
Author(s):  
Kyrsten J Janke ◽  
Linda S Jacobson ◽  
Jolene A Giacinti ◽  
J Scott Weese
Keyword(s):  
Small Sample Size ◽  
Point Of Care ◽  
Small Sample ◽  
Enteric Pathogens ◽  
Test Results ◽  
Viral Dna ◽  
Virus Shedding ◽  
Feline Panleukopenia Virus ◽  
Viral Shedding ◽  
Point Of Care Test

Objectives The aims of this study were to determine the magnitude and duration of fecal viral DNA shedding after diagnosis of feline panleukopenia (FP) in a group of shelter cats using quantitative real-time PCR (qPCR); to assess the utility of a negative point-of-care test or the resolution of diarrhea and systemic signs as proxy measures for qPCR positivity; and to investigate patterns of additional enteric pathogens in relation to feline panleukopenia viral shedding duration. Methods Feline panleukopenia virus (FPV) infection in clinically affected shelter cats was confirmed by a commercial qPCR test. Observations were made on days 0, 3, 7, 14 and 21 post-diagnosis. Fecal flotation, FPV qPCR and the canine parvovirus IDEXX SNAP Parvo ELISA (SNAP) test were performed on fecal samples. Results Forty cats and kittens with confirmed panleukopenia were initially enrolled. Sixteen kittens were sampled until day 14, and 12 were followed to day 21. Median DNA viral copy numbers fell below the diagnostic cut-off by day 7, with 13/16, 6/16, 1/16 and 0/12 testing PCR-positive on days 3, 7, 14 and 21, respectively. The SNAP test was positive in 12/16 kittens on day 0 and only 3/16 on day 3. SNAP test results, diarrhea and systemic signs were inconsistent in relation to qPCR positivity post-diagnosis. Additional enteric pathogens were common. The presence of additional pathogen types was suggestive of a longer PCR shedding duration, but this was not tested statistically owing to the small sample size. Conclusions and relevance These findings suggest that cats should be isolated for at least 14 days after a diagnosis of FP, but that release from isolation after this point is reasonable, in association with a multifaceted infection control strategy. The study findings did not support using SNAP test results, diarrhea or systemic signs as proxy measures for virus shedding.

scholarly journals Comparison between B·R·A·H·M·S PCT direct, a new sensitive point-of-care testing device for rapid quantification of procalcitonin in emergency department patients and established reference methods – a prospective multinational trial

2016 ◽  
Vol 54 (4) ◽  
Author(s):  
Alexander Kutz ◽  
Pierre Hausfater ◽  
Michael Oppert ◽  
Murat Alan ◽  
Eva Grolimund ◽  
...  
Keyword(s):  
Emergency Department ◽  
Diagnostic Accuracy ◽  
Whole Blood ◽  
Point Of Care ◽  
Venous Blood ◽  
Reference Method ◽  
Point Of Care Test ◽  
Emergency Department Patients ◽  
Work Up

AbstractProcalcitonin (PCT) is increasingly being used for the diagnostic and prognostic work up of patients with suspected infections in the emergency department (ED). Recently, B·R·A·H·M·S PCT direct, the first high sensitive point-of-care test (POCT), has been developed for fast PCT measurement on capillary or venous blood samples.This is a prospective, international comparison study conducted in three European EDs. Consecutive patients with suspicion of bacterial infection were included. Duplicate determination of PCT was performed in capillary (fingertip) and venous whole blood (EDTA), and compared to the reference method. The diagnostic accuracy was evaluated by correlation and concordance analyses.Three hundred and three patients were included over a 6-month period (60.4% male, median age 65.2 years). The correlation between capillary or venous whole blood and the reference method was excellent: rThis study found a high diagnostic accuracy and a faster time to result of B·R·A·H·M·S PCT direct in the ED setting, allowing shortening time to therapy and a more wide-spread use of PCT.

Multicentre evaluation of a new point-of-care test for the determination of CK-MB in whole blood

2008 ◽  
Vol 46 (5) ◽  
Author(s):  
Evangelos Giannitsis ◽  
Hannsjörg Baum ◽  
Thomas Bertsch ◽  
Martin Juchum ◽  
Margit Müller-Bardorff ◽  
...  
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Point Of Care Test

scholarly journals Rapid, inexpensive, fingerstick, whole-blood, sensitive, specific, point-of-care test for anti-Toxoplasma antibodies

2018 ◽  
Vol 12 (8) ◽  
pp. e0006536 ◽  
Author(s):  
Joseph Lykins ◽  
Xuan Li ◽  
Pauline Levigne ◽  
Ying Zhou ◽  
Kamal El Bissati ◽  
...  
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Specific Point ◽  
Point Of Care Test

scholarly journals Accuracy and Precision of a Point-of-Care HbA1c Test

2019 ◽  
Vol 14 (5) ◽  
pp. 883-889
Author(s):  
William D. Arnold ◽  
Kenneth Kupfer ◽  
Randie R. Little ◽  
Meera Amar ◽  
Barry Horowitz ◽  
...  
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Venous Blood ◽  
Reference Laboratory ◽  
Test Results ◽  
Blood Samples ◽  
Accuracy And Precision ◽  
The Mean ◽  
Whole Blood Samples ◽  
Highly Correlated

Background: Point-of-care (POC) hemoglobin A1c (HbA1c) testing has advantages over laboratory testing, but some questions have remained regarding the accuracy and precision of these methods. The accuracy and the precision of the POC Afinion™ HbA1c Dx test were investigated. Methods: Samples spanning the assay range were collected from prospectively enrolled subjects at three clinical sites. The accuracy of the POC test using fingerstick and venous whole blood samples was estimated via correlation and bias with respect to values obtained by an NGSP secondary reference laboratory (SRL). The precision of the POC test using fingerstick samples was estimated from duplicate results by calculating the coefficient of variation (CV) and standard deviation (SD), and separated into its components using analysis of variance (ANOVA). The precision of the POC test using venous blood was evaluated from samples run in four replicates on each of three test cartridge lots, twice per day for 10 consecutive days. The SD and CV by study site and overall were calculated. Results: Across the assay range, POC test results from fingerstick and venous whole blood samples were highly correlated with results from the NGSP SRL ( r = .99). The mean bias was −0.021% HbA1c (−0.346% relative) using fingerstick samples and −0.005% HbA1c (−0.093% relative) using venous samples. Imprecision ranged from 0.62% to 1.93% CV for fingerstick samples and 1.11% to 1.69% CV for venous samples. Conclusions: The results indicate that the POC test evaluated here is accurate and precise using both fingerstick and venous whole blood.

scholarly journals Evaluation of a Rapid Whole Blood ELISA for Quantification of Troponin I in Patients with Acute Chest Pain

1999 ◽  
Vol 45 (10) ◽  
pp. 1789-1796 ◽  
Author(s):  
Christopher Heeschen ◽  
Britta U Goldmann ◽  
Lukas Langenbrink ◽  
Guido Matschuck ◽  
Christian W Hamm
Keyword(s):  
Chest Pain ◽  
Whole Blood ◽  
Troponin I ◽  
Point Of Care ◽  
Acute Chest Pain ◽  
Test System ◽  
Healthy Population ◽  
Roc Curve Analysis ◽  
Prognostic Information ◽  
Point Of Care Test

Abstract Background: Troponin I (cTnI) provides important prognostic information in patients with chest pain. We wished to evaluate a rapid, whole-blood analyzer for quantitative point-of-care testing. Methods: A quantitative point-of-care test system (Stratus CS®; Dade-Behring) for cTnI with an incorporated centrifuge was evaluated in 412 patients with chest pain less than 12 h. Results: Results were available within 15 min. CVs were 4.5% at 0.1 μg/L, 4.2% at 0.25 μg/L, and 6.5% at 0.82 μg/L. The detection limit was 0.01 μg/L. The 97.5% percentile in a healthy population was 0.08 μg/L. Based on ROC curve analysis, a threshold of 0.15 μg/L was calculated for the detection of acute myocardial infarction (AMI). With it, sensitivity for the detection of patients with AMI (n = 62) was 63% at arrival and 98% after 4 h (Stratus II®, 48% and 85%, respectively; P <0.01). In 42% of patients with unstable angina (n = 121), cTnI was ≥0.08 μg/L (Stratus II, 28%; P <0.01). During 30 days, death or AMI occurred in 25.5% of these cTnI-positive vs 2.9% of cTnI-negative patients (Stratus II, 29.4% vs 5.8%). Conclusion: The Stratus CS provided better analytical performance and comparable or better prognostic information than the Stratus II.

Clinical Evaluation of a New Point-of-Care System for Chemistry Panel Testing

2019 ◽  
Vol 4 (2) ◽  
pp. 180-192
Author(s):  
Sara A Love ◽  
Karen Schulz ◽  
Penney Stack ◽  
Sharifa Abdirizak ◽  
Maniphone Souriyayong ◽  
...  
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Isotope Dilution Mass Spectrometry ◽  
Method Comparison ◽  
Test Results ◽  
Disease Epidemiology ◽  
Continuous Quality ◽  
Panel Testing ◽  
Point Of Care System ◽  
Blood Specimens

Abstract Background GEM Premier ChemSTAT™ is a point-of-care (POC) system that measures Na+, K+, Ca++, Cl−, glucose, hematocrit, creatinine, blood urea nitrogen (BUN), tCO2, pH, pCO2, and lactate from a single whole blood specimen, providing rapid results in POC settings such as the emergency department (ED). Accurate measurements of creatinine in whole blood and reporting of estimated glomerular filtration rate (eGFR) can minimize adverse effects of contrast-induced nephropathy. Methods Heparinized whole blood specimens from the ED were analyzed on the ChemSTAT by POC staff. Method comparison was performed against the cobas Integra c501 for creatinine, BUN, and tCO2, and against the GEM Premier 4000 for all other analytes. Precision was conducted with whole blood specimens assayed in triplicate over 6 days. Creatinine results from whole blood and plasma were used for eGFR, by isotope dilution mass spectrometry-traceable Modification of Diet in Renal Disease and Chronic Kidney Disease Epidemiology Collaboration equations, and eGFR concordance was assessed. Results Creatinine, BUN, and tCO2 correlated well with plasma samples on the cobas, and all other analytes correlated well with whole blood specimens on the GEM Premier 4000 across the tested sample ranges. The regression slope was 0.951 to 1.047, along with a correlation coefficient (r) of ≥0.982 for all analytes. The pooled within-sample precision was 0% to 2.5% for all analytes. Conclusions ChemSTAT demonstrated a strong correlation with the comparative methods and excellent precision. The system's analytical performance and continuous quality management make it suitable for use in the ED to provide rapid reliable test results, which could minimize the time to treatment and improve ED efficiency.

Whole Blood Multiple Electrode Aggregometry Is a Reliable Point-of-Care Test of Aspirin-Induced Platelet Dysfunction

2009 ◽  
Vol 109 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Csilla Jámbor ◽  
Christian F. Weber ◽  
Konstanze Gerhardt ◽  
Wulf Dietrich ◽  
Michael Spannagl ◽  
...  
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Platelet Dysfunction ◽  
Multiple Electrode Aggregometry ◽  
Point Of Care Test ◽  
Multiple Electrode
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