scholarly journals Capillary Electrophoresis of Serum Proteins. Reproducibility, Comparison with Agarose Gel Electrophoresis and a Review of the Literature

1996 ◽  
Vol 34 (7) ◽  
Author(s):  
Petal A. H. M. Wijnen ◽  
Marja P. van Dieijen-Visser
Keyword(s):  
Capillary Electrophoresis ◽  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Review Of The Literature

Quantitative analysis of serum proteins separated by capillary electrophoresis

1993 ◽  
Vol 39 (4) ◽  
pp. 689-692 ◽  
Author(s):  
J W Kim ◽  
J H Park ◽  
J W Park ◽  
H J Doh ◽  
G S Heo ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Serum Samples ◽  
Polyclonal Gammopathy ◽  
Quantitative Analyses

Abstract The possibility of open tubular capillary electrophoresis for clinical diagnostic use is examined. Capillary electrophoresis was performed in an untreated 50 microns (i.d.) x 100 cm (65 cm to detector) capillary with detection of absorbance at 200 nm. Conditions for the separation of serum proteins without adsorption to the capillary surface were established. Quantitative analyses of serum samples from 38 patients with liver cirrhosis, nephrotic syndrome, or polyclonal gammopathy by capillary electrophoresis were done and the results were compared with those by conventional agarose gel electrophoresis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. All samples were analyzed in duplicate. We evaluated linearity of response, within-run CV, and the correlation between capillary electrophoresis and agarose gel electrophoresis.

scholarly journals Quantification of Serum Proteins Using Capillary Electrophoresis

1995 ◽  
Vol 32 (5) ◽  
pp. 493-497 ◽  
Author(s):  
M A Jenkins ◽  
M D Guerin
Keyword(s):  
Capillary Electrophoresis ◽  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Clinical Laboratory ◽  
Charged Particles ◽  
Protein Electrophoresis ◽  
Agarose Gel Electrophoresis ◽  
Serum Samples ◽  
Routine Clinical Laboratory ◽  
Sample Dilution

Capillary electrophoresis is a technique that can be automated for the separation of charged particles. By investigating suitable sample dilution and injection time and adhering to a strict washing procedure we have been able to quantify paraproteins in serum samples. This has enabled us to use the technique of capillary electrophoresis for the provision of serum protein electrophoresis in a routine clinical laboratory. We present our findings of 260 serum samples, which included 76 samples with paraproteins analysed by both capillary electrophoresis (EC) and high resolution agarose gel electrophoresis (HRAGE). CE was able to detect all the monoclonal bands detected by HRAGE, and, in particular, better able to detect IgA monoclonal bands occurring in the beta region. The major advantages of CE over HRAGE relate to the automated nature of CE with the elimination of the need for a densitometer.

scholarly journals Analysis of serum proteins by agarose gel and capillary electro phoresis

2013 ◽  
Vol 26 (3) ◽  
pp. 267-272
Keyword(s):  
Capillary Electrophoresis ◽  
Serum Protein ◽  
Gel Electrophoresis ◽  
Protein Fraction ◽  
High Speed ◽  
Serum Proteins ◽  
Attractive Alternative ◽  
Automatic Process ◽  
Agarose Gel ◽  
Two Systems

Electrophoresis is a basic technique used to identify disorders of blood serum protein fractions. Agarose gel is the most frequently used medium for routine protein separations. However, capillary electrophoresis seems to be an attractive alternative to gel electrophoresis. The article presents the results of comparative analysis of two systems (Sebia): Hydrasys designed for electrophoretic separations on agarose gel and Minicap for capillary electrophoresis. The purpose of study was to evaluate comparatively the concentrations of each serum protein fraction obtained by gel and capillary electrophoresis and to analyze the correlations between the results obtained by those two systems depending on the concentrations of each protein fraction. The study was carried out in the group of 98 patients, 46 females and 52 males. Despite slight quantitative differences in certain fractions obtained by both methods, capillary electrophoresis offers a fully automatic process of analysis, high speed and efficiency which proves that capillary electrophoresis is appropriate alternative to gel electrophoresis.

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