scholarly journals Influence of Platelet Aggregation Modulators on Cyclic AMP Production in Human Thrombocytes

Folia Medica ◽  
2018 ◽  
Vol 60 (2) ◽  
pp. 241-247
Author(s):  
Krasimir O. Boyanov ◽  
Ana I. Maneva
Keyword(s):  
Platelet Aggregation ◽  
Cyclic Amp ◽  
Ion Transport ◽  
Redox State ◽  
Platelet Aggregation Inhibitors ◽  
Human Platelets ◽  
Signal Pathways ◽  
Phosphatidylinositol 3 Kinase ◽  
Elisa Kit ◽  
Aggregation Inhibitors

Abstract Background: Cyclic AMP is a powerful inhibitor of platelet aggregation. In the present study we examined the effect of platelet aggregation modulators on cyclic AMP content in human thrombocytes. Of the agents we tested, lactoferrin, wortmannin, quercetin and amiloride are platelet aggregation inhibitors, whereas ouabain is a platelet activator. Aim: To investigate the effect of lactoferrin, wortmannin, quercetin, ouabain and amiloride applied alone and in combination with lactoferrin on cyclic AMP production in human platelets. Materials and methods: ‘Direct cAMP ELISA kit’ was used for cyclic AMP determination. Results: The studied modulators, individually or in combination, stimulate cyclic AMP production in platelets. Conclusions: Wortmannin, quercetin, ouabain and amiloride increase cyclic AMP level in human platelets. Lactoferrin also increases cyclic AMP level, but the effect is statistically insignificant, which shows that lactoferrin does not participate directly in the cyclic AMP signaling. Lactoferrin additionally augments the stimulating action of wortmannin, quercetin, ouabain and amiloride on the cyclic AMP production. This probably shows a synergetic interference of lactoferrin in signal pathways along with phosphatidylinositol 3-kinase (wortmannin), quercetin (control over protein kinases, the redox state of the cell and ion transport), ouabain and amiloride (mechanisms of ion transport and phosphorylation).

Platelet Reactions and Immune Processes

1970 ◽  
Vol 23 (01) ◽  
pp. 110-119 ◽  
Author(s):  
F Jobin ◽  
France Tremblay ◽  
M Morissette
Keyword(s):  
Platelet Aggregation ◽  
Platelet Aggregation Inhibitors ◽  
Human Platelets ◽  
Present Knowledge ◽  
Release Reaction ◽  
Cellular Processes ◽  
Cell Release ◽  
Aggregation Inhibitors ◽  
Platelet Release

SummaryWe have studied the effect of chymotrypsin substrates and inhibitors on the aggregation of human platelets by collagen, latex, and epinephrine :1. We have found that platelet aggregation was inhibited by most chymotrypsin substrates and inhibitors which we studied.2. In general, there was a positive correlation between the effectiveness of the compounds as chymotrypsin substrates or inhibitors on one hand, and as platelet aggregation inhibitors on the other hand. However aromatic amino acid derivatives acetylated on the α-amine group were much less effective with platelets than they are with chymotrypsin.3. Chymotrypsin substrates and inhibitors also inhibit the anaphylactic release of histamine. The view is presented that the platelet release reaction and the mast cell release reaction have several common biological and biochemical features.4. The possible role of platelet esterases in platelet thrombogenetic reactions is discussed in the light of the present knowledge of the role of cell bound esterase in several inflammatory or immune cellular processes.

New Platelet Aggregation Inhibitors

1997 ◽  
Vol 17 (01) ◽  
pp. 43-48 ◽  
Author(s):  
R. Verhaeghe ◽  
J. Vermylen
Keyword(s):  
Platelet Aggregation ◽  
Platelet Aggregation Inhibitors ◽  
Aggregation Inhibitors

scholarly journals Platelet Aggregation Inhibitors in a Bhutanese Medicinal Plant, Shug Chher.

1993 ◽  
Vol 41 (9) ◽  
pp. 1604-1607 ◽  
Author(s):  
Kiyomi KAGAWA ◽  
Katsuya TOKURA ◽  
Kiyohisa UCHIDA ◽  
Hisato KAKUSHI ◽  
Tsutomu SHIKE ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Medicinal Plant ◽  
Platelet Aggregation Inhibitors ◽  
Aggregation Inhibitors

scholarly journals ACE and platelet aggregation inhibitors from Tamarix hohenackeri Bunge (host plant of Herba Cistanches) growing in Xinjiang

2014 ◽  
Vol 10 (38) ◽  
pp. 111 ◽  
Author(s):  
Jing Liao ◽  
Yingzhan Tang ◽  
Chengyu Tan ◽  
Hui Ni ◽  
Xueqin Wu ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Host Plant ◽  
Platelet Aggregation Inhibitors ◽  
Aggregation Inhibitors

scholarly journals Comparative Study on the Effect of Carbenicillin, Inhibitors of Platelet Function and Heparin on Experimental Thrombus Formation

1977 ◽  
Author(s):  
R. Zimmermann ◽  
K. Andrassy ◽  
C. Zeltsch ◽  
D. Lange ◽  
F. Hof
Keyword(s):  
Platelet Aggregation ◽  
Bleeding Time ◽  
Thrombus Formation ◽  
Platelet Aggregation Inhibitors ◽  
Venous System ◽  
Arterial System ◽  
Antithrombotic Activity ◽  
Lower Extent ◽  
Aggregation Inhibitors ◽  
Blood Elements

Previous studies documented an impairment of haemostasis by synthetic penicillins (Thromb. Haem. 34: 115, 1976) and penicillin (Lancet II : 1039, 1976). Therefore the antithrombotic activity of synthetic penicillin. (carbenicillin)(C) was compared with that of aspirin (ASA), dipyridamole (DIPY) and heparin in 150 rabbits. Thrombus formation was induced by standardized endothelial lesions. The dose of C was adjusted to a 4.2 fold prolongation of bleeding time, similar to that seen in clinical patients. Analysis and composition of thrombi was done by measurement of incorporation of labeled blood elements (51cr labeled platelets, 125J-fibrinogen and 59Fe labeled red cells). The ‘specific thrombus/blood ratio’ with values of 19.1 and 50.9 (51cr) in venous and arterial thrombi evidenced the significance of platelets in this model. In the venous system C reduced formation of thrombi by 43%, ASA by 34%, ASA and DIPY by 55% and heparin by 90%. In the arterial system C inhibited thrombus formation by 89%, ASA by 15%, ASA and DIPY by 46% and heparin by 60%. It is concluded, that C effectively prevents thrombus formation in the arterial system and to lower extent in the venous system. The results prove the importance of platelets in arterial thrombogenesis and the efficacy of platelet aggregation inhibitors in preventing thrombi in the arterial system. In comparison with other known antiplatelet drugs it seems, that C is the most effective platelet aggregation inhibitor to date.

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