MICROPROPAGATION OF Helianthus maximiliani (Schrader) BY SHOOT APEX CULTURE / MICROPROPAGACION DE Helianthus maximiliani (Schrader) POR EL CULTIVO DE VAINAS DE LOS VASTAGOS / MICROPROPAGATION DE L’Helianthus maximiliani (Schrader) AU MOYEN DE LA CULTURE DE L’APEX DE LA POUSSE

Helia ◽  
2001 ◽  
Vol 24 (34) ◽  
pp. 63-68 ◽  
Author(s):  
Vasić Dragana ◽  
Škorić Dragan ◽  
Alibert Gilbert ◽  
Miklič Vladimir
Keyword(s):  
Silver Nitrate ◽  
Shoot Apex ◽  
Casein Hydrolysate ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Shoot Apices ◽  
Skoog Medium ◽  
Leaf Mesophyll ◽  
Murashige And Skoog Medium

SUMMARYH.maximiliani was micropropagated using culture of shoot apices on modified Murashige and Skoog medium (DV). Further propagation of in vitro grown plants was done by culture of their nodal segments and shoot tips on the same medium supplemented with phloridzin, silver nitrate and casein hydrolysate (DV'). Rooting was induced by dipping the explants into IBA solution prior culture. Viable protoplasts (90%) were isolated from leaf mesophyll. These protoplasts divided (18%) in culture in agarose droplets.

scholarly journals In Vitro Regeneration of Medicinally Important Shrub Carissa Opaca from Shoot Apices and Nodal Segments

2018 ◽  
Vol 3 ◽  
pp. 23-29
Author(s):  
Ali Ahmad ◽  
Bilal Haider Abbasi ◽  
Muhammad Zia
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Genetic Transformation ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Ex Vitro ◽  
Shoot Apices ◽  
Murashige And Skoog Medium ◽  
Carissa Opaca

The study was aimed to develop efficient shoot regeneration fromex vitroexplants ofCarissa opaca, an imperative medicinal reservoir. Shoot apices and nodal segments were inoculated on MS (Murashige and Skoog) medium containing BAP (6-bezyl amino purine) and Kin (Kinetin) alone and in combination with NAA (naphthalene acetic acid) and GA3(Gibberellic acid). Higher concentrations of both cytokinins were found effective for regeneration from both explants. However, gibberellic acid and NAA addition with cytokinin, no persuading results were achieved. The shoot apices were found more effective inin vitroregeneration than nodal segments.The protocol can be effectively used for in vitro multiplication ofC. opaca, genetic transformation, and secondary metabolite production.

scholarly journals Shoot Proliferation and Rooting in Vitro of Pulmonaria

HortScience ◽  
1998 ◽  
Vol 33 (2) ◽  
pp. 339-341 ◽  
Author(s):  
Dennis P. Stimart ◽  
John C. Mather ◽  
Kenneth R. Schroeder
Keyword(s):  
Shoot Proliferation ◽  
Shoot Tips ◽  
Optimum Level ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Ba Genotype

Expanding shoot tips of Pulmonaria `Roy Davidson' and Pulmonaria saccharata `Margery Fish' were cultured in vitro on a modified Murashige and Skoog medium containing BA to establish proliferating cultures for use in comparing BA concentrations on shoot proliferation and rooting. The optimum level for shoot proliferation was 8.8 μm BA. Greatest rooting was on medium without BA. Genotype and time in culture influenced shoot and root counts. Chemical names used: N6-benzyladenine (BA)

scholarly journals Micropropagation of Yellow Passionfruit by Axillary Bud Proliferation

HortScience ◽  
1997 ◽  
Vol 32 (7) ◽  
pp. 1276-1277 ◽  
Author(s):  
Joao L.C. Faria ◽  
Juan Segura
Keyword(s):  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Apices ◽  
Passiflora Edulis ◽  
Multiple Shoot Formation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium

A protocol for in vitro propagation in yellow passionfruit (Passiflora edulis F. flavicarpa Deg) has been developed. Shoot apices from aseptically grown seedlings were used as initial explants. Multiple shoot formation was obtained by placing the explants on solidified Murashige and Skoog medium containing BA. Regenerated shoots were rooted on media without growth regulators. Following conventional procedures, plantlets were transferred to soil with more than 90% success. Chemical name used: N-(phenylmethyl)-lH-purin-6-amine (BA).

scholarly journals REGENERATION OF CITRUS VIA SHOOT APECIES

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1112a-1112
Author(s):  
Suzanne M.D. Rogers ◽  
Kalyani Dias ◽  
David Byrne
Keyword(s):  
Growth Regulators ◽  
Shoot Tips ◽  
Sour Orange ◽  
Soil Medium ◽  
Skoog Medium ◽  
Murashige And Skoog Medium

Viral damage is a major problem in citrus. As most citrus are asexually propagated, it is necessary to have an alternative way of regenerating virus-free plants from infected plants. Shoot apicies are the most suitable explant material for this purpose because that part of the plant is virus-free. Fifty sour orange shoot tips and 22 Swingle shoot tips, 1 mm - 1.5 mm long, were excised from in vitro germinated seedlings and cultured on semisolid Murashige and Skoog medium, without growth regulators, containing 0.2 % Gelrite. After 8-10 weeks, shoots and leaves developed in 68'% of the sour orange explants, and in 77% of the Swingle explants. Some explants produced roots, after 11-12 weeks, and could be removed from culture and established in soil medium.

Cryopreservation of in vitro-grown shoot tips of strawberry by the vitrification method using aluminium cryo-plates

2011 ◽  
Vol 10 (1) ◽  
pp. 14-19 ◽  
Author(s):  
Shin-ichi Yamamoto ◽  
Kuniaki Fukui ◽  
Tariq Rafique ◽  
Nayyar Iqbal Khan ◽  
Carlos Roman Castillo Martinez ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Sucrose Solution ◽  
Shoot Tips ◽  
New Method ◽  
Fragaria Ananassa ◽  
Alginate Gel ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Strawberry Cultivars

Cryopreservation using an aluminium cryo-plate was successfully applied to in vitro-grown strawberry (Fragaria × ananassa Duch.) shoot tips. The shoots were cold-hardened at 5°C for 3 weeks with an 8-h photoperiod. The shoot tips (1.5–2.0 mm × 0.5–1.0 mm) were dissected from the shoot and pre-cultured at 5°C for 2 d on Murashige and Skoog medium containing 2 M glycerol and 0.3 M sucrose. The pre-cultured shoot tips were placed on the aluminium cryo-plate containing ten wells embedded in alginate gel. Osmoprotection was performed by immersing the cryo-plates in a loading solution (2 M glycerol and 0.8 M sucrose) for 30 min at 25°C. Dehydration was performed by immersing the cryo-plates in plant vitrification solution 2 for 50 min at 25°C. Then, the cryo-plate with shoot tips was transferred into an uncapped cryotube that was held on a cryo-cane and directly immersed into liquid nitrogen (LN). After storage in LN, shoot tips attached to the cryo-plate were directly immersed into 2 ml of a 1 M sucrose solution for regeneration. Using this procedure, the average regrowth level of vitrified shoot tips of 15 strawberry cultivars reached 81%. This new method has many advantages and will facilitate the cryostorage of strawberry germplasm.

scholarly journals Critical vitrification steps towards survival of Garcinia hombroniana (Clusiaceae) shoot tips after cryopreservation

2018 ◽  
Vol 66 (3) ◽  
pp. 1314
Author(s):  
Norafarain Sulong ◽  
Nurul Farhana Shahabudin ◽  
Normah Mohd Noor
Keyword(s):  
Water Content ◽  
Liquid Nitrogen ◽  
Shoot Tips ◽  
The Other ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Vitrification Solution ◽  
Cryopreservation Protocol ◽  
Recovery Medium

A cryopreservation protocol was developed for in vitro shoot tips of Garcinia hombroniana using the vitrification technique. Four critical steps in the technique were investigated, namely preculture, loading, dehydration with Plant Vitrification Solution 2 (PVS2), and unloading. Shoot tips precultured for 48 hr gave significantly higher survival (75 %) compared to 24 hr preculture (50 %) after cryopreservation. Treatment with 1 M glycerol plus 0.4 M sucrose as a loading solution gave higher survival (45.83 %) compared to the other treatments (0.4 M sucrose + 2 M glycerol; 0.4 M sucrose). Shoot tips dehydrated with PVS2 for 25 min gave the highest survival after immersion in liquid nitrogen. Stepwise PVS2 treatment for 15 min with 50 % PVS2 followed by 10 min with 100 % PVS2 solution improved survival of the shoot tips after cryopreservation (41.67 %). Murashige and Skoog medium with 0.4 M sucrose gave significantly higher survival (66.67 %) than MS with 1.2 M sucrose (25 %) as an unloading solution. Water content was shown to decrease throughout the whole vitrification steps from 6.83 ± 1.66 g g-1 dw for fresh shoot tips down to 2.93 ± 0.28 g g-1 dw after PVS2 treatment. Further study on each step including recovery medium is required to improve the survival. Nevertheless, the present study showed the potential of using the vitrification technique for cryopreservation of G. hombroniana.

In vitro plant regeneration from leaf expiants of Lycopersicon esculentum (tomato)

1976 ◽  
Vol 54 (21) ◽  
pp. 2409-2414 ◽  
Author(s):  
R. M. Behki ◽  
S. M. Lesley
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Growth Regulators ◽  
In Vitro Plant Regeneration ◽  
Leaf Discs ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Napthaleneacetic Acid ◽  
In Vitro Plant

Leaf discs from 15 mutant clones of tomato were tested for their morphogenetic response in Murashige and Skoog medium supplemented with 12 combinations of the growth regulators napthaleneacetic acid (NAA) and benzylaminopurine (BA) and 4 combinations of NAA and zeatin. The results show that either callus, shoots, roots, or shoots and roots can be produced depending upon the hormone concentrations and ratios. Plants were regenerated from 12 of the 15 varieties tested.

scholarly journals In vitro propagation of muskmelon (Cucumis melo L.) from nodal segments, shoot tips and cotyledonary nodes

2015 ◽  
Vol 41 ◽  
pp. 71-77
Author(s):  
S. Parvin ◽  
M. Kausar ◽  
M. Enamul Haque ◽  
M. Khalekuzzaman ◽  
B. Sikdar ◽  
...  
Keyword(s):  
Cucumis Melo ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Ms Medium ◽  
Cotyledonary Nodes ◽  
Cucumis Melo L

A rapid and efficient protocol is outlined for in vitro propagation of muskmelon(Cucumis melo L.) Shoot tips, nodal segments and cotyledonary nodes from invitro grown seedlings were used as explants. The explants were inoculated on MS medium fortified with different combinations and concentrations of growthregulators viz., BAP, NAA, GA3 and IBA for multiple shoot regeneration.Effective result was found on MS medium supplemented with 2.0 mg/l BAP, inwhich 90% and 70% cultures induced multiple shoots from nodal segments andshoot tip explants, respectively. Whereas, 70% cultures of cotyledonary nodeswere found to induced shoots on MS medium with 1.5 mg/l BAP + 0.1 mg/l GA3. In vitro regenerated shoots were subcultured on half strength MS mediumsupplemented with different concentrations of IBA and NAA for successful rootinduction and the effective result (up to 70%) was found in medium with 1 mg/lIBA. Well rooted in vitro grown plantlets were acclimatized in sandy soil, whereas 70% plantlets survived

Sign in / Sign up

Export Citation Format

Share Document