Modeling Study of Crude Oil Enhanced Removal in a Two-Liquid Phase Partitioning Bioreactor

Abstract A combined process of solvent extraction and two-phase biodegradation was carried out to remove crude oil from water by mixed cultures, where silicone oil was selected as the organic solvent due to its biocompatibility and non-biodegradability. The crude oil removal and cell growth was experimentally studied. A simple model that combined steady mass transfer equations and dynamic growth kinetics of suspended cells was suggested to follow the entire process. Under the conditions studied, complete removal of crude oil from water was achieved at initial crude oil concentration of 5,000 mg/L. Results revealed that the proposed model satisfactorily described the process as long as crude oil level in the cell medium did not exceed the toxicity limit of suspended cells.

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Evaluation of Parallel-Series Configurations of Two-Phase Partitioning Biotrickling Filtration and Biotrickling Filtration for Treating Styrene Gas-Phase Emissions

Sustainability ◽

10.3390/su12176740 ◽

2020 ◽

Vol 12 (17) ◽

pp. 6740 ◽

Cited By ~ 1

Author(s):

Pau San-Valero ◽

Javier Álvarez-Hornos ◽

Pablo Ferrero ◽

Josep M. Penya-Roja ◽

Paula Marzal ◽

...

Keyword(s):

High Throughput Sequencing ◽

Silicone Oil ◽

Sharp Decrease ◽

Complete Removal ◽

Industrial Applications ◽

Biotrickling Filter ◽

Gaseous Emissions ◽

Two Phase ◽

Phase Partitioning ◽

In Series

The removal of styrene from industrial representative gaseous emissions was studied using two reactors connected in series: a two-phase partitioning biotrickling filter (TPPB-BTF) and a conventional biotrickling filter (BTF). The system was operated under industrial conditions, which included steady and transient conditions and intermittent spraying. Silicone oil was used in the TPPB-BTF with a quantity as low as 25 mL L−1, promoting a faster start-up compared to the BTF. By working at a styrene loading of 30 g m−3 h−1, nearly complete removal efficiency (RE) was obtained. In addition, the removal was not adversely impacted by using non-steady emission patterns such as overnight shutdowns (97% RE) and oscillating concentrations (95% RE), demonstrating its viability for industrial applications. After 2 months from inoculation, two additional configurations (reverse series BTF + TPPB-BTF and parallel) were tested, showing the series configuration as the best approach to consistently achieve RE > 95%. After 51 days of operation, high throughput sequencing revealed a sharp decrease in the bacterial diversity. In both reactors, the microorganisms belonging to the Comamonadaceae family were predominant and other styrene degraders such as Pseudomonadaceae proliferated preferably in the first reactor.

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Effect of surfactants and biomass on the gas/liquid mass transfer in an aqueous-silicone oil two-phase partitioning bioreactor usingRhodococcus erythropolisT902.1 to remove VOCs from gaseous effluents

Journal of Chemical Technology & Biotechnology ◽

10.1002/jctb.2172 ◽

2009 ◽

Vol 84 (9) ◽

pp. 1274-1283 ◽

Cited By ~ 13

Author(s):

Jean-Marc Aldric ◽

Sébastien Gillet ◽

Frank Delvigne ◽

Christophe Blecker ◽

Frédéric Lebeau ◽

...

Keyword(s):

Mass Transfer ◽

Silicone Oil ◽

Two Phase ◽

Phase Partitioning ◽

Liquid Mass ◽

Partitioning Bioreactor ◽

Liquid Mass Transfer ◽

Two Phase Partitioning Bioreactor

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Study on Mass Transfer of Isopropylbenzene and Oxygen in a Two-Phase Partitioning Bioreactor in the Presence of Silicone Oil

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-008-8480-0 ◽

2009 ◽

Vol 153 (1-3) ◽

pp. 67-79 ◽

Cited By ~ 16

Author(s):

Jean-Marc Aldric ◽

Jean-Paul Lecomte ◽

Philippe Thonart

Keyword(s):

Mass Transfer ◽

Silicone Oil ◽

Two Phase ◽

Phase Partitioning ◽

Partitioning Bioreactor ◽

Two Phase Partitioning Bioreactor

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Aerobic phenanthrene biodegradation in a two-phase partitioning bioreactor

Water Science & Technology ◽

10.2166/wst.2005.0272 ◽

2005 ◽

Vol 52 (8) ◽

pp. 265-271 ◽

Cited By ~ 9

Author(s):

R. Muñoz ◽

C. Rolvering ◽

B. Guieysse ◽

B. Mattiasson

Keyword(s):

Silicone Oil ◽

Liquid Volume ◽

Agitation Rate ◽

Two Phase ◽

Phase Partitioning ◽

Partitioning Bioreactor ◽

Photosynthetic Oxygenation ◽

Mechanical Aeration ◽

Phenanthrene Biodegradation ◽

Two Phase Partitioning Bioreactor

The aerobic degradation of phenanthrene by a Pseudomonas migulae strain under classical mechanical aeration and under photosynthetic oxygenation (using a Chlorella sorokiniana strain) in a two-phase partitioning bioreactor (TPPB) constructed with silicone oil as organic phase was investigated. When traditional mechanical aeration was used, an increase in the aeration and/or in the agitation rate enhanced phenanthrene biodegradation. Thus, phenanthrene removal rates (based on the total liquid volume of cultivation) ranged from 22±1 to 36±2mg/lh at 100rpm and 1vvm and 400rpm and 3vvm, respectively. On the other hand, during phenanthrene biodegradation using the algal-bacterial microcosm a maximum rate of 8.1±1.2mg/lh at 200rpm and 8000 lux of illuminance was achieved.

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