Reinigung und Charakterisierung zweier Proteine des Adenovirus Typ 2

1965 ◽  
Vol 20 (8) ◽  
pp. 747-752 ◽  
Author(s):  
Kurt Köhler

Two soluble antigens produced in KB-cells after infection with Adenovirus type 2 were identified with constituents of the virion itself. Purification and concentration of the two proteins (named A- and C-Protein according to PEREIRA) is described. The sedimentation rate of the A-Protein is 11.5 S, which gives a molecular weight of 310 000, and the corresponding data for the C-Protein are 5.5 S and 81 000 molecular weight. The A-Proteins of different types, 2, 3 and 5 are physically similar, but serologically composed of parts with similar and parts with type-specific differences.

Virology ◽  
1976 ◽  
Vol 74 (2) ◽  
pp. 386-393 ◽  
Author(s):  
Frederick Varricchio ◽  
Lynne M. Sehulster ◽  
Karel Raška

1966 ◽  
Vol 17 (2) ◽  
pp. 428-439 ◽  
Author(s):  
Paul R. Reich ◽  
Bernard G. Forget ◽  
Sherman M. Weissman ◽  
James A. Rose

Cell ◽  
1976 ◽  
Vol 7 (4) ◽  
pp. 585-593 ◽  
Author(s):  
Hans Söderlund ◽  
Ulf Pettersson ◽  
Björn Vennström ◽  
Lennart Philipson ◽  
Michael B. Mathews

Author(s):  
C. N. SUN

The presence of non-viral paracrystalline protein in cells infected with adenovirus type 5 (2) and adenovirus type 2 (4) has been reported.The present studies revealed adenovirus type 12 produced: (a) paracrystalline bodies in KB cells morphologically similar to that induced by adenovirus type 5 and (b) small crystalline lattices which consisted of tubular subunits.The sequential developmental studies of adenovirus type 12 were made in KB cells and in cell lines derived from human origin. In the infected KB cells, the intranuclear patches and stipplings were clearly seen beginning at 18th hour of inoculation (Fig. 1).


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