Intracellular forms of adenovirus DNA VII. Excision of viral sequences from cellular DNA in adenovirus type 2-infected KB cells

Virology ◽  
1978 ◽  
Vol 85 (2) ◽  
pp. 557-567 ◽  
Author(s):  
Knut Baczko ◽  
Rainer Neumann ◽  
Walter Doerfler
Keyword(s):  
Adenovirus Type ◽  
Kb Cells ◽  
Cellular Dna ◽  
Viral Sequences ◽  
Adenovirus Type 2

Replicating DNA of Adenovirus Type 2

1974 ◽  
Vol 52 (3) ◽  
pp. 181-189 ◽  
Author(s):  
Danielle Bourgaux-Ramoisy ◽  
Jean Robin ◽  
Pierre Bourgaux
Keyword(s):  
Molecular Form ◽  
Deae Cellulose ◽  
Adenovirus Type ◽  
Kb Cells ◽  
Viral Dna ◽  
Short Pulses ◽  
Radioactive Label ◽  
Cellular Dna ◽  
Adenovirus Type 2

We have investigated the nature of the DNA synthesized in KB cells 24 h after infection with adenovirus type 2 (ad. 2). Cellular DNA synthesis being strongly depressed, a new molecular form of ad. 2 DNA could be detected in such cells in addition to mature viral DNA. This new form, referred to as DNA-R, is preferentially labelled after short pulses of 3H-thymidine, while the radioactive label can be chased into mature viral DNA. The sedimentation properties of DNA-R are those expected for linear replicating molecules having 1–2 times the molecular weight of mature viral DNA. As a result of being partly single-stranded, these molecules can be separated from mature viral DNA by chromatography on benzoylated-naphthoylated DEAE-cellulose. Purified DNA-R was examined under the electron microscope and found to consist of Y-shaped molecules. Thus DNA-R has the properties expected for linear monomeric molecules of ad. 2 DNA replicating undirectionally.

scholarly journals Adenovirus type 2 activates cell cycle-dependent genes that are a subset of those activated by serum.

1985 ◽  
Vol 5 (11) ◽  
pp. 2936-2942 ◽  
Author(s):  
H T Liu ◽  
R Baserga ◽  
W E Mercer
Keyword(s):  
Cell Cycle ◽  
Dna Synthesis ◽  
Thymidine Kinase ◽  
Histone H3 ◽  
Adenovirus Type ◽  
3T3 Cells ◽  
Cycle Dependent ◽  
Cellular Dna ◽  
Adenovirus Type 2

We have studied a panel of 10 genes and cDNA sequences that are expressed in a cell cycle-dependent manner in different types of cells from different species and that are inducible by different mitogens. These include five sequences (c-myc, 4F1, 2F1, 2A9, and KC-1) that are preferentially expressed in the early part of the G1 phase, three genes (ornithine decarboxylase, p53, and c-rasHa) preferentially expressed in middle or late G1, and two genes (thymidine kinase and histone H3) preferentially expressed in the S phase of the cell cycle. We have studied the expression of these genes in nonpermissive (tsAF8) and semipermissive (Swiss 3T3) cells infected with adenovirus type 2. Under the conditions of these experiments, adenovirus type 2 infection stimulates cellular DNA synthesis in both tsAF8 and 3T3 cells. However, four of the five early G1 genes (c-myc, 4F1, KC-1, and 2A9) and one of the late G1 genes (c-ras) are not induced by adenovirus infection, although they are strongly induced by serum. The other sequences (2F1, ornithine decarboxylase, p53, thymidine kinase, and histone H3) are activated by both adenovirus and serum. We conclude that the cell cycle-dependent genes activated by adenovirus 2 are a subset of the cell cycle-dependent genes activated by serum. The data suggest that the mechanisms by which serum and adenovirus induce cellular DNA synthesis are not identical.

Adenovirus type 2 activates cell cycle-dependent genes that are a subset of those activated by serum

1985 ◽  
Vol 5 (11) ◽  
pp. 2936-2942
Author(s):  
H T Liu ◽  
R Baserga ◽  
W E Mercer
Keyword(s):  
Cell Cycle ◽  
Dna Synthesis ◽  
Thymidine Kinase ◽  
Histone H3 ◽  
Adenovirus Type ◽  
3T3 Cells ◽  
Cycle Dependent ◽  
Cellular Dna ◽  
Adenovirus Type 2

We have studied a panel of 10 genes and cDNA sequences that are expressed in a cell cycle-dependent manner in different types of cells from different species and that are inducible by different mitogens. These include five sequences (c-myc, 4F1, 2F1, 2A9, and KC-1) that are preferentially expressed in the early part of the G1 phase, three genes (ornithine decarboxylase, p53, and c-rasHa) preferentially expressed in middle or late G1, and two genes (thymidine kinase and histone H3) preferentially expressed in the S phase of the cell cycle. We have studied the expression of these genes in nonpermissive (tsAF8) and semipermissive (Swiss 3T3) cells infected with adenovirus type 2. Under the conditions of these experiments, adenovirus type 2 infection stimulates cellular DNA synthesis in both tsAF8 and 3T3 cells. However, four of the five early G1 genes (c-myc, 4F1, KC-1, and 2A9) and one of the late G1 genes (c-ras) are not induced by adenovirus infection, although they are strongly induced by serum. The other sequences (2F1, ornithine decarboxylase, p53, thymidine kinase, and histone H3) are activated by both adenovirus and serum. We conclude that the cell cycle-dependent genes activated by adenovirus 2 are a subset of the cell cycle-dependent genes activated by serum. The data suggest that the mechanisms by which serum and adenovirus induce cellular DNA synthesis are not identical.

Reinigung und Charakterisierung zweier Proteine des Adenovirus Typ 2

1965 ◽  
Vol 20 (8) ◽  
pp. 747-752 ◽  
Author(s):  
Kurt Köhler
Keyword(s):  
Molecular Weight ◽  
Sedimentation Rate ◽  
Adenovirus Type ◽  
Kb Cells ◽  
C Protein ◽  
Different Types ◽  
Adenovirus Type 2

Two soluble antigens produced in KB-cells after infection with Adenovirus type 2 were identified with constituents of the virion itself. Purification and concentration of the two proteins (named A- and C-Protein according to PEREIRA) is described. The sedimentation rate of the A-Protein is 11.5 S, which gives a molecular weight of 310 000, and the corresponding data for the C-Protein are 5.5 S and 81 000 molecular weight. The A-Proteins of different types, 2, 3 and 5 are physically similar, but serologically composed of parts with similar and parts with type-specific differences.

Studies on synthesis and structure of virus-coded low molecular weight RNA in adenovirus type 2-infected KB cells

Virology ◽  
1976 ◽  
Vol 74 (2) ◽  
pp. 386-393 ◽  
Author(s):  
Frederick Varricchio ◽  
Lynne M. Sehulster ◽  
Karel Raška
Keyword(s):  
Molecular Weight ◽  
Adenovirus Type ◽  
Low Molecular Weight ◽  
Kb Cells ◽  
Adenovirus Type 2

Electron Microscopy of Cells Infected with Adenovirus Type 12

1970 ◽  
Vol 28 ◽  
pp. 336-337
Author(s):  
C. N. SUN
Keyword(s):  
Electron Microscopy ◽  
Cell Lines ◽  
Adenovirus Type ◽  
Developmental Studies ◽  
Human Origin ◽  
Kb Cells ◽  
Adenovirus Type 5 ◽  
Made In ◽  
Adenovirus Type 2

The presence of non-viral paracrystalline protein in cells infected with adenovirus type 5 (2) and adenovirus type 2 (4) has been reported.The present studies revealed adenovirus type 12 produced: (a) paracrystalline bodies in KB cells morphologically similar to that induced by adenovirus type 5 and (b) small crystalline lattices which consisted of tubular subunits.The sequential developmental studies of adenovirus type 12 were made in KB cells and in cell lines derived from human origin. In the infected KB cells, the intranuclear patches and stipplings were clearly seen beginning at 18th hour of inoculation (Fig. 1).

RNA of low molecular weight in KB cells infected with adenovirus type 2

1966 ◽  
Vol 17 (2) ◽  
pp. 428-439 ◽  
Author(s):  
Paul R. Reich ◽  
Bernard G. Forget ◽  
Sherman M. Weissman ◽  
James A. Rose
Keyword(s):  
Molecular Weight ◽  
Adenovirus Type ◽  
Low Molecular Weight ◽  
Kb Cells ◽  
Adenovirus Type 2
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