Isolation and Characterization of Cytotoxic Diterpenoid Isomers from Propolis

1997 ◽  
Vol 52 (9-10) ◽  
pp. 702-704 ◽  
Author(s):  
Tetsuya Matsuno ◽  
Yasuyuki Matsumoto ◽  
Masahiro Saito ◽  
Junji Morikawa
Keyword(s):  
Methanol Extract ◽  
Carcinoma Cell ◽  
Molecular Formula ◽  
Cell Cytotoxicity ◽  
Isolation And Characterization ◽  
Clerodane Diterpenoids ◽  
Human Hepatocellular Carcinoma Cell ◽  
Hepatocellular Carcinoma Cell ◽  
Brazilian Propolis

The methanol extract of Brazilian propolis was fractionated by HPLC, based on HuH13 (human hepatocellular carcinoma) cell cytotoxicity assay. Two isomers of diterpenoid with a molecular formula of C20H30O3 (MW: 318.46) were isolated. The structures of these colorless compounds were determined as clerodane diterpenoids (I, 15-oxo-3, 13Z-kolavadien-17-oic acid; II, 15-oxo-3Z, 13E-kolavadien-n-oic acid)

Establishment and characterization of a new human hepatocellular carcinoma cell line

In Vitro ◽  
1984 ◽  
Vol 20 (6) ◽  
pp. 493-504 ◽  
Author(s):  
Lun He ◽  
Kurt J. Isselbacher ◽  
Jack R. Wands ◽  
Howard M. Goodman ◽  
Chiaho Shih ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Human Hepatocellular Carcinoma ◽  
Carcinoma Cell Line ◽  
Hepatocellular Carcinoma Cell Line ◽  
Human Hepatocellular Carcinoma Cell ◽  
Hepatocellular Carcinoma Cell

A New Benzo-γ-pyran Derivative Isolated from Propolis

1998 ◽  
Vol 53 (11-12) ◽  
pp. 1037-1039 ◽  
Author(s):  
Tetsuya Matsuno ◽  
Masahiro Saito ◽  
Yasuyuki Matsumoto ◽  
Junji Morikawa
Keyword(s):  
Hepatocellular Carcinoma ◽  
Methanol Extract ◽  
Cytotoxicity Assay ◽  
Human Hepatocellular Carcinoma ◽  
Molecular Formula ◽  
Cell Cytotoxicity ◽  
Propenoic Acid ◽  
Artepillin C ◽  
Brazilian Propolis ◽  
Pyran Derivative

Abstract The methanol extract of Brazilian propolis was fractionated by HPLC, based on human hepatocellular carcinoma (HuH 13) cell cytotoxicity assay. A new benzo-y-pyran derivative (PM-3) with a molecular formula of C19H22O3 (MW: 298.38) was isolated. The structure of this colorless compound was determined as 3-[2-dimethyl-8-(3-methyl-2-butenyl) benzopy-ran]-6 -propenoic acid. This compound was chemically synthesized by cyclisation of artepillin C (3-[4-hydroxy-3,5-bis (3-methyl-2-butenyl) phenyl]-2-propenoic acid).

SYNTHESIS AND CYTOTOXICITY OF POLYHYDROXYLATED CHOLESTEROL DERIVATIVES

2018 ◽  
Vol 56 (4) ◽  
pp. 467
Author(s):  
Thu Thuy Thi Tran ◽  
Ha Thi Dinh ◽  
Phương Lan Doan ◽  
Long Quoc Pham ◽  
Quang Dai Ngo
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Addition Compound ◽  
Hep G2 ◽  
Physical Methods ◽  
Hepatocellular Carcinoma Cell Line ◽  
Human Hepatocellular Carcinoma Cell ◽  
Hepatocellular Carcinoma Cell

Eight polyhydroxylated cholesterol derivatives (1-8) were prepared from cholesterol, using oxidative reagents as SeO2, OsO4/NMO, HCOOH/H2O2 and BH3/ H2O2. Their structures were elucidated by using physical methods including NMR 1D and 2D. These compounds were evaluated against two cancer cell lines (Hep-G2, T98). Compounds 2, 4 and 8 inhibits human hepatocellular carcinoma cell line (Hep-G2) with IC50 4.69, 4.98 and 2.89 µg/mL, respectively. In addition, compound 8 exhibited strong cytotoxicity against T98 cell line (glioblastoma) with IC50 = 2.28 μM.

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