Discriminatory Power of RAPD,PCR-RFLP and Southern Blot Analyses of ureCD or ureA Gene Probes on Helicobacter pylori Isolates
2002 ◽
Vol 57
(5-6)
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pp. 516-521
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Keyword(s):
Rapd Pcr
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The genetic diversity of 33 Nigerian Helicobacter pylori isolates were studied using RAPD, PCR-RFLP and Southern blot analysis of ureA or ureCD gene probes. RAPD was able to distinguish the following number of isolates using the primers 3880 : 5ʹ-AAGAGCCCGT-3ʹ (28), 3881 :5ʹ-AACGCGCAAC-3ʹ (33) and OPH8 :5ʹ-GAAACACCCC-3ʹ (25). Southern blot analysis using the ureCD probe was also able to distinguish the 12 isolates tested into ten different patterns. The PCR-RFLP technique distinguished all 33 isolates into six types. In conclusion, considering typeability, discriminatory power, and convenience, RAPD with the 3881 primer was considered the most useful technique.
Keyword(s):
1998 ◽
pp. 77-87
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2016 ◽
Vol 477
(3)
◽
pp. 317-321
◽
Keyword(s):
1995 ◽
pp. 361-368
◽
1988 ◽
Vol 8
(6)
◽
pp. 2328-2334
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Keyword(s):