scholarly journals Clastogenic Effect of Carthamus lanatus L. (Asteraceae)

2003 ◽  
Vol 58 (11-12) ◽  
pp. 833-836
Author(s):  
Margarita Topashka-Ancheva ◽  
Rilka Taskova ◽  
Nedjalka Handjieva ◽  
Bozhanka Mikhova ◽  
Helmut Duddeck
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberration ◽  
Chromosome Aberrations ◽  
Water Extract ◽  
Positive Control ◽  
Aerial Parts ◽  
Chromosome Aberration Assay ◽  
Different Types ◽  
Clastogenic Effect ◽  
Bone Marrow Chromosome

Abstract The clastogenic effect of total dichloromethane, methanol and water extracts, four bioactive fractions and three individual constituents from Carthamus lanatus aerial parts were evaluated in mice by bone marrow chromosome aberration assay with mitomycin C as positive control. Significant differences in the percentage of aberrant mitosis of the extracts were observed. The dichloromethane extract exhibited a considerable clastogenic effect and the water extract a negligible one. Different types of chromosome aberrations and time-dependant effects for the active fractions and individual compounds were found.

scholarly journals Hydroxytyrosol: lack of clastogenicity in a bone marrow chromosome aberration study in rats

2014 ◽  
Vol 7 (1) ◽  
pp. 923
Author(s):  
Laurie C Dolan ◽  
Hana Hofman-Hüther ◽  
Nicole Amann
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberration ◽  
Bone Marrow Chromosome

Genotoxicity Evaluation of Dimethoate to Experimental Mice by Micronucleus, Chromosome Aberration Tests, and Comet Assay

2011 ◽  
Vol 31 (1) ◽  
pp. 78-85 ◽  
Author(s):  
Imen Ayed-Boussema ◽  
Karima Rjiba ◽  
Nourhène Mnasri ◽  
Amal Moussa ◽  
Hassen Bacha
Keyword(s):  
Bone Marrow ◽  
Comet Assay ◽  
Chromosome Aberration ◽  
Bone Marrow Cells ◽  
Mouse Bone Marrow ◽  
Dependent Manner ◽  
Organophosphate Insecticide ◽  
Delayed Effect ◽  
Chromosome Aberration Assay ◽  
Marrow Cells

Dimethoate (DM) is an organophosphate insecticide with numerous uses on field and agricultural crops and ornamentals. Data concerning DM-acute genotoxicity are controversial and knowledge on its delayed effect is limited. For this reason, we aimed to further explore DM genotoxicity resulting from subchronic intoxication of experimental mice. Thus, DM was administered to mice at doses ranging from 1 to 30 mg/kg body weight for a period of 30 consecutive days. There was a significant increase ( P < .05) in the frequency of micronucleated bone marrow cells following DM administration. Furthermore, the chromosome aberration assay revealed a significant increase in the percentage of chromosome abnormalities in a dose-dependent manner. Dimethoate was also found to induce significant DNA damage in mouse bone marrow cells as assessed by the comet assay. Altogether, our results showed that, after a subchronic exposure, DM was a genotoxic compound in experimental mice.

Evaluation of genotoxicity of pan masala employing chromosomal aberration and micronucleus assay in bone marrow cells of the mice

2009 ◽  
Vol 25 (7) ◽  
pp. 467-471 ◽  
Author(s):  
BN Mojidra ◽  
K. Archana ◽  
AK Gautam ◽  
Y. Verma ◽  
BC Lakkad ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberration ◽  
Chromosomal Aberration ◽  
Bone Marrow Cells ◽  
Micronucleus Assay ◽  
Control Group ◽  
Genotoxic Potential ◽  
Polychromatic Erythrocytes ◽  
Significant Difference ◽  
Marrow Cells

Pan masala is commonly consumed in south-east Asian and other oriental countries as an alternate of tobacco chewing and smoking. Genotoxic potential of pan masala (pan masala plain and pan masala with tobacco known as gutkha) was evaluated employing chromosome aberration (CA) and micronucleus (MN) assay in vivo. Animals were exposed to three different doses (0.5%, 1.5% and 3%) of pan masala plain (PMP) and gutkha (PMT) through feed for a period of 6 months and micronucleus and chromosomal aberrations were studied in the bone marrow cells. Induction of mean micronuclei in polychromatic erythrocytes (MNPCE) and normochromatic erythrocyte (MNNCE) was higher in both types of pan masala treated groups with respect to control group. Both pan masala plain and gutkha treatment significantly induced the frequency of MNPCE and MNNCE in the bone marrow cells, indicating the genotoxic potential. Furthermore, slight decline in the ratio of polychromatic erythrocytes to normochromatic erythrocytes was also noticed, suggesting the cytotoxic potential even though the ratio was statistically non significant. A dose-dependent, significant increase in chromosome aberration was observed in both types of pan masala treated mice with respect to control. However, no significant difference in micronucleus and chromosomal aberration induction was noticed between two types of pan masala exposed (PMP and PMT) groups. Results suggest that both types of pan masala, i.e. plain and gutkha, have genotoxic potential.

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