Relative genotoxicity of trichloroacetic acid (TCA) as revealed by different cytogenetic assays: bone marrow chromosome aberration, micronucleus and sperm-head abnormality in the mouse

1987 ◽  
Vol 188 (3) ◽  
pp. 215-221 ◽  
Author(s):  
S.P. Bhunya ◽  
B.C. Behera
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberration ◽  
Trichloroacetic Acid ◽  
Sperm Head ◽  
Sperm Head Abnormality ◽  
Bone Marrow Chromosome

scholarly journals Clastogenic Effect of Carthamus lanatus L. (Asteraceae)

2003 ◽  
Vol 58 (11-12) ◽  
pp. 833-836
Author(s):  
Margarita Topashka-Ancheva ◽  
Rilka Taskova ◽  
Nedjalka Handjieva ◽  
Bozhanka Mikhova ◽  
Helmut Duddeck
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberration ◽  
Chromosome Aberrations ◽  
Water Extract ◽  
Positive Control ◽  
Aerial Parts ◽  
Chromosome Aberration Assay ◽  
Different Types ◽  
Clastogenic Effect ◽  
Bone Marrow Chromosome

Abstract The clastogenic effect of total dichloromethane, methanol and water extracts, four bioactive fractions and three individual constituents from Carthamus lanatus aerial parts were evaluated in mice by bone marrow chromosome aberration assay with mitomycin C as positive control. Significant differences in the percentage of aberrant mitosis of the extracts were observed. The dichloromethane extract exhibited a considerable clastogenic effect and the water extract a negligible one. Different types of chromosome aberrations and time-dependant effects for the active fractions and individual compounds were found.

scholarly journals Hydroxytyrosol: lack of clastogenicity in a bone marrow chromosome aberration study in rats

2014 ◽  
Vol 7 (1) ◽  
pp. 923
Author(s):  
Laurie C Dolan ◽  
Hana Hofman-Hüther ◽  
Nicole Amann
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberration ◽  
Bone Marrow Chromosome

Evaluation of genotoxicity of pan masala employing chromosomal aberration and micronucleus assay in bone marrow cells of the mice

2009 ◽  
Vol 25 (7) ◽  
pp. 467-471 ◽  
Author(s):  
BN Mojidra ◽  
K. Archana ◽  
AK Gautam ◽  
Y. Verma ◽  
BC Lakkad ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberration ◽  
Chromosomal Aberration ◽  
Bone Marrow Cells ◽  
Micronucleus Assay ◽  
Control Group ◽  
Genotoxic Potential ◽  
Polychromatic Erythrocytes ◽  
Significant Difference ◽  
Marrow Cells

Pan masala is commonly consumed in south-east Asian and other oriental countries as an alternate of tobacco chewing and smoking. Genotoxic potential of pan masala (pan masala plain and pan masala with tobacco known as gutkha) was evaluated employing chromosome aberration (CA) and micronucleus (MN) assay in vivo. Animals were exposed to three different doses (0.5%, 1.5% and 3%) of pan masala plain (PMP) and gutkha (PMT) through feed for a period of 6 months and micronucleus and chromosomal aberrations were studied in the bone marrow cells. Induction of mean micronuclei in polychromatic erythrocytes (MNPCE) and normochromatic erythrocyte (MNNCE) was higher in both types of pan masala treated groups with respect to control group. Both pan masala plain and gutkha treatment significantly induced the frequency of MNPCE and MNNCE in the bone marrow cells, indicating the genotoxic potential. Furthermore, slight decline in the ratio of polychromatic erythrocytes to normochromatic erythrocytes was also noticed, suggesting the cytotoxic potential even though the ratio was statistically non significant. A dose-dependent, significant increase in chromosome aberration was observed in both types of pan masala treated mice with respect to control. However, no significant difference in micronucleus and chromosomal aberration induction was noticed between two types of pan masala exposed (PMP and PMT) groups. Results suggest that both types of pan masala, i.e. plain and gutkha, have genotoxic potential.

scholarly journals Inheritance of sperm head abnormality types in mice – the role of the Y chromosome

1976 ◽  
Vol 28 (2) ◽  
pp. 189-198 ◽  
Author(s):  
Halina Krzanowska
Keyword(s):  
Relative Frequency ◽  
Relative Proportion ◽  
Inbred Strains ◽  
Nuclear Material ◽  
Sperm Head ◽  
Total Percentage ◽  
Inbred Strains Of Mice ◽  
Reciprocal Differences ◽  
Sperm Head Abnormality ◽  
Seven Generations

SUMMARYFour inbred strains of mice were used, differing in the total percentages of spermatozoa with abnormal heads (KE, 22·1%; C57, 26·4%; KP, 7·7%; CBA, 5·5%) and in the frequency distribution of abnormality types, as divided into four arbitrary classes. The most variable class 2 (narrow heads with canals inside the nuclear material) accounted for 47% of all abnormalities in KE strain, was common in CBA (29%) and almost missing in KP and C57 strains. F1 hybrids from the diallel crosses of these strains exhibited highly significant heterosis effects and significant reciprocal differences in the total percentage of abnormalities. The relative frequency of class 2 ranked in F1 hybrids in a similar order as calculated from the mid-parental values. After seven generations of backcrosses performed to introduce the Y chromosome from CBA to the genetical background of the KE strain, the total percentage of abnormalities was significantly reduced, although the relative proportion of class 2 was similar to that in KE strain. Also the Y chromosome from C57 strain, introduced into the genetical background of KE strain, caused a significant reduction of total abnormalities, but again the relative frequency of class 2 was not affected. It is concluded that the Y chromosome plays an important role in determining the total percentage of sperm head abnormalities, but does not seem to be involved in influencing specific abnormality types.

scholarly journals Factor responsible for spermatozoan abnormality located on the Y chromosome in mice

1969 ◽  
Vol 13 (1) ◽  
pp. 17-24 ◽  
Author(s):  
H. Krzanowska
Keyword(s):  
Karyotype Analysis ◽  
Inbred Strains ◽  
Fertilization Rate ◽  
Sperm Head ◽  
Inbred Strains Of Mice ◽  
Heterosis Effect ◽  
The Mean ◽  
The Difference ◽  
Sperm Head Abnormality ◽  
Seven Generations

Two inbred strains of mice differing in the mean percentage of spermatozoa with abnormal heads were used: KE (16·1%) and CBA (5·9%). The F1 resulting from the crosses exhibited a heterosis effect, while in the backcrosses an obvious segregation of genotypes was observed; both generations showed a reciprocal difference, depending on the source of the Y chromosome. The character of sperm head abnormality seems to be polygenically determined, one of the genes being located on chromosome Y.Seven generations of backcrosses were performed in which the Y chromosome from CBA was introduced to the genetical background of the KE strain. In the seventh generation 10·2% of abnormal spermatozoa were found, which is significantly lower than in the KE strain. The difference shows the net effect of the Y-linked locus. A correlated difference was found in the fertilization rate, indicating that a factor influencing male fertility is located on chromosome Y. It does not seem to influence the shape of normal spermatozoan heads.Karyotype analysis did not reveal gross abnormalities in the KE strain.

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