Two New Methods Monitoring Kinetics of Hydrolysis of Acetylcholine and Acetylthiocholine
2005 ◽
Vol 60
(11-12)
◽
pp. 943-946
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Keyword(s):
Ph Stat
◽
Hydroxylamine and HPLC methods, measuring in vitro kinetics of enzymatic hydrolysis of acetylcholine or acetylthiocholine by cholinesterases, are described. The hydroxylamine method determines the dependence of substrate concentration vs. time, the HPLC method is able to measure simultaneously the time dependences of substrate and both primary products, choline or thiocholine, and acetic acid. Practical determinations are shown, comparison with known (above all Ellman’s and pH-stat) methods, advantages and disadvantages are discussed.
2007 ◽
Vol 109
(1-3)
◽
pp. 179-181
◽
1985 ◽
Vol 3
(4)
◽
pp. 375-379
1971 ◽
Vol 36
(12)
◽
pp. 4115-4117
◽
1981 ◽
Vol 46
(5)
◽
pp. 1229-1236
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Keyword(s):
1959 ◽
Vol 234
(6)
◽
pp. 1550-1554
Keyword(s):
1989 ◽
Vol 102
(2-3)
◽
pp. 268-272
◽
1990 ◽
Vol 37
(5)
◽
pp. 479-487
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