scholarly journals Combination of immunocytochemistry and radioligand receptor assay to identify beta-adrenergic receptor subtypes on astroglia in vitro

1984 ◽  
Vol 4 (6) ◽  
pp. 1598-1606 ◽  
Author(s):  
PA Trimmer ◽  
T Evans ◽  
MM Smith ◽  
TK Harden ◽  
KD McCarthy
Keyword(s):  
Adrenergic Receptor ◽  
Receptor Subtypes ◽  
Beta Adrenergic Receptor ◽  
Beta Adrenergic ◽  
Receptor Assay ◽  
Radioligand Receptor Assay

scholarly journals The beta-adrenergic receptor adenylate cyclase complex of Rauscher murine erythroleukemia cells and its response to erythropoietin-induced differentiation

Blood ◽  
1984 ◽  
Vol 64 (1) ◽  
pp. 84-90 ◽  
Author(s):  
AJ Sytkowski ◽  
CJ Kessler
Keyword(s):  
Adenylate Cyclase ◽  
Adrenergic Receptor ◽  
Developmental Process ◽  
Adenosine Monophosphate ◽  
Beta Adrenergic Receptor ◽  
Beta Adrenergic ◽  
Erythroleukemia Cells ◽  
Murine Erythroleukemia ◽  
Murine Erythroleukemia Cells

Abstract Rauscher murine erythroleukemia cells, grown continuously in vitro, undergo erythroid differentiation in response to the hormone erythropoietin. Therefore, they serve as an important model system with which to examine critical biochemical aspects of this developmental process. Intact, uninduced Rauscher cells possess a functional beta- adrenergic receptor-adenylate cyclase complex. The adrenergic agonists, isoproterenol, epinephrine, and norepinephrine, exhibited activation constants (Kact) of 0.1, 0.5, and 20 mumol/L, respectively. Thus, the beta-receptor-cyclase complex of Rauscher cells is apparently one of the most sensitive of all erythroid cells reported thus far. The epinephrine-stimulated cyclic adenosine monophosphate (cAMP) response was inhibited by propranolol, alprenolol, and hydroxybenzylpindolol, with inhibition constants (KI) of 3.8, 2.2, and 0.1 nmol/L, respectively. Using [125I]-iodohydroxybenzylpindolol as ligand, uninduced Rauscher cells were shown to possess 1,100 receptors/cell, with an equilibrium dissociation constant (KD) of 400 pmol/L. Erythropoietin, but not dimethylsulfoxide, induction caused a specific increase in receptor density to 3,300/cell on differentiating Rauscher cells. This is the first demonstration of membrane receptor regulation by erythropoietin that may be important in the complex interplay of hormonal effects during erythropoiesis.

Plasma membrane-specific deficiency in cardiac beta-adrenergic receptor in streptozocin-diabetic rats

1989 ◽  
Vol 257 (2) ◽  
pp. E127-E132 ◽  
Author(s):  
A. Kashiwagi ◽  
Y. Nishio ◽  
Y. Saeki ◽  
Y. Kida ◽  
M. Kodama ◽  
...  
Keyword(s):  
Cell Surface ◽  
Adrenergic Receptor ◽  
Insulin Treatment ◽  
Diabetic Rats ◽  
Cell Surface Receptor ◽  
Beta Adrenergic Receptor ◽  
Beta Adrenergic ◽  
Receptor Concentration ◽  
Cgp 12177

Cell surface [3H]CGP 12177 binding sites in 10-wk streptozocin-diabetic rats decreased by 41% (P less than 0.01) compared with that in the control rats. In contrast, there was no difference in the total cell receptor concentration between the control and the diabetic rats, which was measured by hydrophobic antagonist [125I]-iodocyanopindolol binding. Forty-eight-hour in vivo insulin treatment significantly (P less than 0.05) increased cell surface beta-adrenergic receptor concentration by 37% above that in diabetic rats without any change in total receptor concentration in the cells. However in vitro treatment of 8 nM insulin, 33 mM glucose, or 10 mM 3-hydroxybutyrate for 2 h showed no effect on [3H]CGP 12177 binding. In contrast, 10 microM isoproterenol-dependent decrease and the recovery of cell surface receptors after the removal of the agonist were significantly (P less than 0.01) impaired in diabetic rats compared with those of control rats. These results indicate that only cell surface beta-adrenergic receptors decrease in diabetic rats, which may be associated with abnormalities in the receptor distribution. The decrease in cell surface receptor number closely associates with the diabetic state and is reversed by the short-term insulin treatment.

Alterations in bilateral distribution of beta-adrenergic receptor subtypes in schizophrenia and suicide

1989 ◽  
Vol 25 (7) ◽  
pp. A197 ◽  
Author(s):  
Nedra Lexow ◽  
Roman Artymyshyn ◽  
Manual Cassanova ◽  
Andrew Winokur ◽  
Joel Kleinman ◽  
...  
Keyword(s):  
Adrenergic Receptor ◽  
Receptor Subtypes ◽  
Beta Adrenergic Receptor ◽  
Beta Adrenergic ◽  
Bilateral Distribution

scholarly journals Beta adrenergic receptor subtypes in human brain

1987 ◽  
Vol 43 ◽  
pp. 268
Author(s):  
Takashi Taniguchi ◽  
Shun Shimohama ◽  
Motohatsu Fujiwara ◽  
Masakuni Kameyama
Keyword(s):  
Human Brain ◽  
Adrenergic Receptor ◽  
Receptor Subtypes ◽  
Beta Adrenergic Receptor ◽  
Beta Adrenergic
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