scholarly journals In Vivo Neuronal Subtype-Specific Targets of Atoh1 (Math1) in Dorsal Spinal Cord

2011 ◽  
Vol 31 (30) ◽  
pp. 10859-10871 ◽  
Author(s):  
H. C. Lai ◽  
T. J. Klisch ◽  
R. Roberts ◽  
H. Y. Zoghbi ◽  
J. E. Johnson
Keyword(s):  
Spinal Cord ◽  
Dorsal Spinal Cord ◽  
Neuronal Subtype ◽  
Dorsal Spinal

Positive N-methyl-d-aspartate receptor modulation by selective glycine transporter-1 inhibition in the rat dorsal spinal cord in vivo

Neuroscience ◽  
2004 ◽  
Vol 126 (2) ◽  
pp. 381-390 ◽  
Author(s):  
K.J Whitehead ◽  
S.M Pearce ◽  
G Walker ◽  
H Sundaram ◽  
D Hill ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Dorsal Spinal Cord ◽  
Receptor Modulation ◽  
Glycine Transporter 1 ◽  
Aspartate Receptor ◽  
Glycine Transporter ◽  
Dorsal Spinal

Sonic hedgehog signaling is required during the appearance of spinal cord oligodendrocyte precursors

Development ◽  
1999 ◽  
Vol 126 (11) ◽  
pp. 2419-2429 ◽  
Author(s):  
D.M. Orentas ◽  
J.E. Hayes ◽  
K.L. Dyer ◽  
R.H. Miller
Keyword(s):  
Spinal Cord ◽  
Sonic Hedgehog ◽  
Ventricular Zone ◽  
Developmental Period ◽  
Dorsal Spinal Cord ◽  
Shh Signaling ◽  
Oligodendrocyte Precursors ◽  
Dorsal Spinal

Spinal cord oligodendrocyte precursors arise in the ventral ventricular zone as a result of local signals. Ectopic oligodendrocyte precursors can be induced by sonic hedgehog (Shh) in explants of chick dorsal spinal cord over an extended developmental period. The role of Shh during normal oligodendrocyte development is, however, unclear. Here we demonstrate that Shh is localized to the ventral spinal cord immediately prior to, and during the appearance of oligodendrocyte precursors. Continued expression of Shh is required for the appearance of spinal cord oligodendrocyte precursors as neutralization of Shh signaling both in vivo and in vitro during a defined developmental period blocked their emergence. The inhibition of oligodendrocyte precursor emergence in the absence of Shh signaling was not the result of inhibiting precursor cell proliferation, and the neutralization of Shh signaling after the emergence of oligodendrocyte precursors had no effect on the appearance of additional cells or their subsequent differentiation. Similar concentrations of Shh induce motor neurons and oligodendrocytes in dorsal spinal cord explants. However, in explants from early embryos the motor neuron lineage is preferentially expanded while in explants from older embryos the oligodendrocyte lineage is preferentially expanded.

scholarly journals Regeneration of dorsal spinal cord neurons after injury via in situ NeuroD1-mediated astrocyte-to-neuron conversion

2019 ◽  
Author(s):  
Brendan Puls ◽  
Yan Ding ◽  
Fengyu Zhang ◽  
Mengjie Pan ◽  
Zhuofan Lei ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Dorsal Horn ◽  
Cell Fate ◽  
High Efficiency ◽  
Reactive Astrocytes ◽  
Dorsal Spinal Cord ◽  
Spinal Cord Neurons ◽  
Cord Injury ◽  
Dorsal Spinal

AbstractSpinal cord injury (SCI) often leads to impaired motor and sensory functions, partially because the injury-induced neuronal loss cannot be easily replenished through endogenous mechanisms. In vivo neuronal reprogramming has emerged as a novel technology to regenerate neurons from endogenous glial cells by forced expression of neurogenic transcription factors. We have previously demonstrated successful astrocyte-to-neuron conversion in mouse brains with injury or Alzheimer’s disease by overexpressing a single neural transcription factor NeuroD1 via retroviruses. Here we demonstrate regeneration of dorsal spinal cord neurons from reactive astrocytes after SCI via adeno-associated virus (AAV), a more clinically relevant gene delivery system. We find that NeuroD1 converts reactive astrocytes into neurons in the dorsal horn of stab-injured spinal cord with high efficiency (∼95%). Interestingly, NeuroD1-converted neurons in the dorsal horn mostly acquire glutamatergic neuronal subtype, expressing spinal cord-specific markers such as Tlx3 but not brain-specific markers such as Tbr1, suggesting that the astrocytic lineage and local microenvironment affect the cell fate of conversion. Electrophysiological recordings show that the NeuroD1-converted neurons can functionally mature and integrate into local spinal cord circuitry by displaying repetitive action potentials and spontaneous synaptic responses. We further show that NeuroD1-mediated neuronal conversion can occur in the contusive SCI model, allowing future studies of evaluating this reprogramming technology for functional recovery after SCI. In conclusion, this study may suggest a paradigm shift for spinal cord repair using in vivo astrocyte-to-neuron conversion technology to generate functional neurons in the grey matter.

Idiopathic dorsal spinal cord herniation perforating the lamina: a case report and review of the literature

2021 ◽  
Author(s):  
Haruki Funao ◽  
Satoshi Nakamura ◽  
Kenshi Daimon ◽  
Norihiro Isogai ◽  
Yutaka Sasao ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Case Report ◽  
Review Of The Literature ◽  
Dorsal Spinal Cord ◽  
Spinal Cord Herniation ◽  
Dorsal Spinal

Spinal integration of antidromic mediated cutaneous vasodilation during dorsal spinal cord stimulation in the rat

1999 ◽  
Vol 260 (3) ◽  
pp. 173-176 ◽  
Author(s):  
Kirk W. Barron ◽  
John E. Croom ◽  
Crystal A. Ray ◽  
Margaret J. Chandler ◽  
Robert D. Foreman
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Stimulation ◽  
Dorsal Spinal Cord ◽  
Cutaneous Vasodilation ◽  
Dorsal Spinal
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