Dry after-ripening, light, cold stratification and temperature effects on seed germination of Primula poissonii from Yunnan, China

Primula poissonii, an attractive wild plant growing in the subalpine/alpine region of southwest China, has low seed germination in cultivation. This study attempted to improve seed germination by testing the effect of several treatments including dry after-ripening (DAR), light, cold stratification (CS) and temperature gradient treatments. DAR increased germination at 15/5 and 25/15°C, as compared with fresh seeds. DAR seeds germinated significantly better (> 80%) at higher temperatures (20-28°C) than at lower (10°C, < 20%; 15°C, < 30%) and extreme high temperatures (30°C, < 55%; 32°C, 0%). Incubation at alternating temperature (25/15°C) did not significantly improve germination; whereas at 15/5°C germination increased significantly, compared with the corresponding constant temperature (20 and 10°C, respectively). DAR seeds had a strict light requirement at all temperatures. As DAR and CS are sufficient to break seed dormancy, the seeds of P. poissonii appear to have non-deep PD. For non-dormant cold-stratified seeds, the estimated Tb and thermal time (θ50) were 2.3°C and 74.1°Cd, respectively.

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Influence of Light and Temperature on Seed Germination of Flame Azalea

Journal of Environmental Horticulture ◽

10.24266/0738-2898-7.3.109 ◽

1989 ◽

Vol 7 (3) ◽

pp. 109-111 ◽

Cited By ~ 1

Author(s):

Asiah A. Malek ◽

Frank A. Blazich ◽

Stuart L. Warren ◽

James E. Shelton

Keyword(s):

Seed Germination ◽

Continuous Illumination ◽

Light Requirement ◽

Alternating Temperature

Abstract Seeds of flame azalea [Rhododendron calendulaceum (Michx.) Torr.] were germinated at 25°C (77°F) and 25°/15°C (77°/59°F) with daily photoperiods of 0, ½, ½ twice daily, 1, 2, 4, 8, 12 and 24 hr. Seeds exhibited an obligate light requirement. At 25°C (77°F), increasing photoperiods increased germination with maximum germination (85%) occurring by day 12 under continuous illumination. The alternating temperature of 25°/15°C (77°/59°F) enhanced germination when light was limiting. At this temperature, maximum germination of 84 to 91% was reached by day 24 for all photoperiods ≥½ hr although at photoperiods ≥4 hr, comparable germination was noted at day 18.

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Seed Germination of Five Populations of Rhododendron vaseyi: Influence of Light and Temperature

Journal of Environmental Horticulture ◽

10.24266/0738-2898-28.3.166 ◽

2010 ◽

Vol 28 (3) ◽

pp. 166-172

Author(s):

Cary J. Hebert ◽

Frank A. Blazich ◽

Anthony V. LeBude

Keyword(s):

Seed Germination ◽

Seed Viability ◽

Continuous Light ◽

Moderate Level ◽

Total Darkness ◽

Light Requirement ◽

Alternating Temperature ◽

Growing Conditions ◽

Entire Distribution

Abstract Seeds from five populations of Rhododendron vaseyi A. Gray (pinkshell azalea), representing the entire distribution of the species, were germinated at 25C (77F) or an 8/16-hr thermoperiod of 30/20C (86/68F) with daily photoperiods at each temperature of 0 (total darkness), 8, 12, or 24-hr (continuous light). Germination was recorded every 3 days for 30 days. Responses to light and temperature of all populations were similar. Light was required for germination regardless of temperature. As photoperiod increased, germination increased for all populations with the alternating temperature partially compensating for the light requirement. The highest cumulative germination for all populations ranged from 51 to 67% and was achieved at 30/20C with a 24-hr photoperiod. These germination percentages, although at a moderate level, were due in part to rigorous cleaning and grading of seeds collected across a broad range of plants and growing conditions prior to initiation of the study, suggesting seed viability of R. vaseyi is inherently low.

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Bleaching and cold stratification can break dormancy and improve seed germination in Cyperaceae

Aquatic Botany ◽

10.1016/j.aquabot.2019.103128 ◽

2019 ◽

Vol 158 ◽

pp. 103128 ◽

Cited By ~ 3

Author(s):

Sergey Rosbakh ◽

Lisa Hülsmann ◽

Irina Weinberger ◽

Maxi Bleicher ◽

Peter Poschlod

Keyword(s):

Seed Germination ◽

Cold Stratification ◽

Improve Seed Germination

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Effect of Pretreatments on Seed Germination of Prunus mahaleb L.

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4027887 ◽

2012 ◽

Vol 40 (2) ◽

pp. 183 ◽

Cited By ~ 4

Author(s):

Elias PIPINIS ◽

Elias MILIOS ◽

Olga MAVROKORDOPOULOU ◽

Christina GKANATSIOU ◽

Maria ASLANIDOU ◽

...

Keyword(s):

Sulfuric Acid ◽

Seed Germination ◽

Gibberellic Acid ◽

Seed Dormancy ◽

Mechanical Resistance ◽

Cold Stratification ◽

Long Period ◽

Prunus Mahaleb ◽

Sexual Propagation ◽

Improve Seed Germination

Sexual propagation of Prunus mahaleb is difficult due to seed dormancy. To overcome dormancy and maximize germination, various pretreatments have been applied, including stratification (warm and cold), gibberellic acid (GA3), sulfuric acid scarification (AS), and endocarp removal. The results show that warm stratification (WS) prior to cold stratification (CS) does not improve seed germination and a long period of WS (3 months) is disastrous for germination. CS alone (up to 4 months) has been found to hasten and increase seed germination. Pretreatment of the seeds with exogenous GA3, during the CS period, has been observed to result in significantly higher seed germination. AS of seeds for 45 minutes prior to GA3 (1000 ppm for 24 hours) plus CS (up to 1 month) pretreatment has been considered to reduce the mechanical resistance of endocarp and improve germination. However, extended time of AS (180 minutes) prior to GA3 plus CS pretreatment has been found to harm the seeds. The removal of endocarp has been noted to significantly improve germination. Seeds without endocarp, which were pretreated with GA3 (1000 or 2000 ppm for 24 hours) and then cold stratified for 1 month, have been noted to exhibit the highest germination percentages.

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Factors affecting eastern black nightshade (Solanum ptycanthum) seed germination

Weed Science ◽

10.1614/ws-04-168r2.1 ◽

2005 ◽

Vol 53 (5) ◽

pp. 651-656 ◽

Cited By ~ 18

Author(s):

Jingkai Zhou ◽

Edward L. Deckard ◽

Calvin G. Messersmith

Keyword(s):

Seed Germination ◽

Environmental Factors ◽

Prolonged Exposure ◽

Short Exposure ◽

Cold Stratification ◽

Light Requirement ◽

Factors Affecting ◽

Eastern Black Nightshade ◽

Solanum Ptycanthum ◽

Black Nightshade

Effects of environmental factors, especially light, on eastern black nightshade seed germination were studied under growth chamber conditions. Germination occurred only when temperatures were 20 C or higher for 12 to 24 h d−1. Optimum germination occurred within the constant temperature range of 28 to 33 C or with alternating temperatures of 30/25 and 35/30 C. In the dark, eastern black nightshade germination was poor except at high alternating temperatures of 30/25 and 35/30 C. Seed germination was enhanced by light when temperatures were suboptimal. Imbibition was a prerequisite for response of eastern black nightshade seed to light. The light requirement for eastern black nightshade germination was partially overcome by cold stratification, gibberellic acid (GA3), and high or alternating temperatures. Germination of eastern black nightshade seed was promoted by short exposure to sunlight but was inhibited by prolonged exposure, especially when irradiance was high.

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Seed dormancy and germination characteristics of Acer cinnamomifolium, a plant species endemic to China

Seed Science and Technology ◽

10.15258/sst.2021.49.3.01 ◽

2021 ◽

Author(s):

Cui-Fang Tang ◽

Yu-Chen Zhao ◽

Lin-Feng Ou ◽

Cai-Hong Zhong ◽

Song-Quan Song

Keyword(s):

Seed Germination ◽

Plant Species ◽

Seed Dormancy ◽

Environmental Changes ◽

Effect Of Temperature ◽

Cold Stratification ◽

Dormancy Release ◽

Temperature Regimes ◽

Germination Characteristics ◽

Alternating Temperature

Seed dormancy is an adaptive response of plants to environmental changes during long-term evolution. It plays an important role in plant survival and propagation. Acer cinnamomifolium, a plant species endemic to China, is an excellent garden and street tree species. However, dormancy and germination characteristics of A. cinnamomifolium seeds are not currently known. In the present paper, the mature seeds were used as experimental materials and we investigated the effect of temperature including alternating temperature, light, after-ripening, stratification and phytohormone on seed dormancy release and germination. After being incubated for 30 days, 0 to 10% of the seeds germinated in constant temperature (10, 15, 20, 25 and 30°C), while in alternating temperature regimes (20/10, 25/15 and 30/20°C), seed germination was < 35%. Dormancy release and germination of seeds can be increased by after-ripening, cold stratification and phytohormone treatments. We concluded that A. cinnamomifolium seeds have a non-deep physiological dormancy. Cold stratification at 4°C for 30 days and incubation thereafter at 20°C were found to be optimal for dormancy release and high seed germination.

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Influence of Light and Temperature on Seed Germination of Mountain Laurel

Journal of Environmental Horticulture ◽

10.24266/0738-2898-7.4.161 ◽

1989 ◽

Vol 7 (4) ◽

pp. 161-162 ◽

Cited By ~ 1

Author(s):

Asiah A. Malek ◽

Frank A. Blazich ◽

Stuart L. Warren ◽

James E. Shelton

Keyword(s):

Seed Germination ◽

Continuous Illumination ◽

Light Requirement ◽

Kalmia Latifolia ◽

Alternating Temperature ◽

Mountain Laurel

Abstract Seeds of mountain laurel (Kalmia latifolia L.) were germinated at 2.5°C (77°F) and 25°/15°C (77°/59°F) with daily photoperiods of 0, ½, ½ twice daily, 1, 2, 4, 8, 12, and 24 hr. Seeds exhibited an obilgate light requirement. At 25°C (77°F), increasing photoperiods increased germination with maximum germination (90%) occuring by day 18 under continuous illumination. The alternating temperature of 25°/15°C (77°/59°F) enhanced germination when light was limiting. At this temperature, maximum germination of 86 to 87% was reached by day 24 for photoperiods ≥ 8 hr.

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