scholarly journals Gas Phase Characterization of the Noncovalent Quaternary Structure of Cholera Toxin and the Cholera Toxin B Subunit Pentamer

2006 ◽  
Vol 90 (9) ◽  
pp. 3246-3254 ◽  
Author(s):  
Jonathan P. Williams ◽  
Daniel C. Smith ◽  
Brian N. Green ◽  
Brian D. Marsden ◽  
Keith R. Jennings ◽  
...  
Keyword(s):  
Cholera Toxin ◽  
Gas Phase ◽  
Quaternary Structure ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Phase Characterization

scholarly journals Biochemical and immunological characterization of an ETEC CFA/I adhesin cholera toxin B subunit chimera

PLoS ONE ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. e0230138 ◽  
Author(s):  
Michael G. Jobling ◽  
Steven T. Poole ◽  
Fatima Rasulova-Lewis ◽  
Aisling O’Dowd ◽  
Annette L. McVeigh ◽  
...  
Keyword(s):  
Cholera Toxin ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Immunological Characterization ◽  
Cholera Toxin B ◽  
B Subunit

Immunological characterization of a rotavirus-neutralizing epitope fused to the cholera toxin B subunit

Gene ◽  
1993 ◽  
Vol 133 (2) ◽  
pp. 227-232 ◽  
Author(s):  
Ramón A. González ◽  
Joaquín Sánchez ◽  
Jan Holmgren ◽  
Susana López ◽  
Carlos F. Arias
Keyword(s):  
Cholera Toxin ◽  
Cholera Toxin B Subunit ◽  
Neutralizing Epitope ◽  
Toxin B ◽  
Immunological Characterization ◽  
Cholera Toxin B ◽  
B Subunit

scholarly journals Spray-Dried Formulation of Epicertin, a Recombinant Cholera Toxin B Subunit Variant That Induces Mucosal Healing

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 576
Author(s):  
Micaela A. Reeves ◽  
Joshua M. Royal ◽  
David A. Morris ◽  
Jessica M. Jurkiewicz ◽  
Nobuyuki Matoba ◽  
...  
Keyword(s):  
Gastric Acid ◽  
Cholera Toxin ◽  
Oral Formulation ◽  
Size Exclusion ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Enteric Coated ◽  
Spray Dried

Epicertin (EPT) is a recombinant variant of the cholera toxin B subunit, modified with a C-terminal KDEL endoplasmic reticulum retention motif. EPT has therapeutic potential for ulcerative colitis treatment. Previously, orally administered EPT demonstrated colon epithelial repair activity in dextran sodium sulfate (DSS)-induced acute and chronic colitis in mice. However, the oral dosing requires cumbersome pretreatment with sodium bicarbonate to conserve the acid-labile drug substance while transit through the stomach, hampering its facile application in chronic disease treatment. Here, we developed a solid oral formulation of EPT that circumvents degradation in gastric acid. EPT was spray-dried and packed into enteric-coated capsules to allow for pH-dependent release in the colon. A GM1-capture KDEL-detection ELISA and size-exclusion HPLC indicated that EPT powder maintains activity and structural stability for up to 9 months. Capsule disintegration tests showed that EPT remained encapsulated at pH 1 but was released over 180 min at pH 6.8, the approximate pH of the proximal colon. An acute DSS colitis study confirmed the therapeutic efficacy of encapsulated EPT in C57BL/6 mice upon oral administration without gastric acid neutralization pretreatment compared to vehicle-treated mice (p < 0.05). These results provide a foundation for an enteric-coated oral formulation of spray-dried EPT.

scholarly journals Chemical and immunochemical studies on the receptor binding domain of cholera toxin B subunit.

1985 ◽  
Vol 260 (23) ◽  
pp. 12528-12534
Author(s):  
D S Ludwig ◽  
R K Holmes ◽  
G K Schoolnik
Keyword(s):  
Cholera Toxin ◽  
Receptor Binding ◽  
Binding Domain ◽  
Cholera Toxin B Subunit ◽  
Receptor Binding Domain ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit

Evidence that cholera toxin B subunit (CTb) can be avidly taken up and transported by fibers of passage

1995 ◽  
Vol 674 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Sheng Chen ◽  
Gary Aston-Jones
Keyword(s):  
Cholera Toxin ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit

Synthesis of Lactose Dendrimers and Multivalency Effects in Binding to the Cholera Toxin B Subunit

2001 ◽  
Vol 2001 (24) ◽  
pp. 4685 ◽  
Author(s):  
Ioannis Vrasidas ◽  
Nico J. de Mol ◽  
Rob M. J. Liskamp ◽  
Roland J. Pieters
Keyword(s):  
Cholera Toxin ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit
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