ANALYSIS OF SOLVOLYZABLE STEROIDS IN HUMAN PLASMA BY COMBINED GAS CHROMATOGRAPHY - MASS SPECTROMETRY
ABSTRACT Solvolyzable steroids in human peripheral plasma were separated by chromatography on Sephadex LH-20 into two fractions having the elution volumes of mono- and disulphates, respectively. The steroid conjugates in the latter fraction had the mobility of steroid disulphates on TLC. After solvolysis in ethyl acetate acidified with sulphuric acid the steroids were analyzed by gas chromatography and combined gas chromatography - mass spectrometry. Cholesterol, dehydroepiandrosterone, androsterone, epiandrosterone, aetiocholanolone, pregnenolone, pregn-5-ene-3β,20α-diol, androst-5-ene-3β,17β-diol, androst-5-ene-3β,17α-diol and an androstanediol, most probably 5α-androstane-3α,17β-diol were found in the monosulphate fraction. Androst-5-ene-3β,17β-diol, androst-5-ene-3β,17α-diol and pregn-5-ene-3β,20α-diol were found in the disulphate fraction. The ratio of C19 to C21 diols was higher in the disulphate than in the monosulphate fraction.