RADIO-LIGAND PROCEDURES FOR ESTROGEN ASSAY IN NORMAL AND PREGNANCY PLASMA

1970 ◽  
Vol 65 (1_Suppl) ◽  
pp. S291-S304 ◽  
Author(s):  
Stanley G. Korenman ◽  
Dan Tulchinsky ◽  
L. William Eaton
Keyword(s):  
Column Chromatography ◽  
Activated Charcoal ◽  
Binding Protein ◽  
Large Numbers ◽  
17Β Estradiol ◽  
Estrogen Binding ◽  
Specific Reagent

ABSTRACT Radio-ligand assay techniques suitable for measurement of unconjugated estrone and 17β-estradiol concentrations in plasma from normal and pregnant individuals are described. The specific estrogen binding protein of rabbit uterus is employed as specific reagent and activated charcoal is used as non-specific adsorbant to separate bound from free radioactive estrogen. Steroids of interest are separated by column chromatography on celite. By these techniques, large numbers of samples can be assayed with speed and precision for both estrone and 17β-estradiol.

Estrogen Binding Protein of Rat Liver

1975 ◽  
Vol 2 (3) ◽  
pp. 273-280 ◽  
Author(s):  
P. Viladiu ◽  
C. Delgado ◽  
J. Pensky ◽  
O. H. Pearson
Keyword(s):  
Rat Liver ◽  
Binding Protein ◽  
Estrogen Binding

scholarly journals Removal of 17β-Estradiol by Activated Charcoal Supported Titanate Nanotubes (TNTs@AC) through Initial Adsorption and Subsequent Photo-Degradation: Intermediates, DFT calculation, and Mechanisms

Water ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2121
Author(s):  
Taobo Huang ◽  
Baozhu Pan ◽  
Haodong Ji ◽  
Wen Liu
Keyword(s):  
Activated Charcoal ◽  
Density Functional ◽  
Capillary Condensation ◽  
Uv Light ◽  
Density Functional Theory Calculation ◽  
Titanate Nanotubes ◽  
Photo Degradation ◽  
Photocatalytic Ability ◽  
17Β Estradiol ◽  
Initial Adsorption

A low-cost composite of activated charcoal supported titanate nanotubes (TNTs@AC) was developed via the facile hydrothermal method to remove the 17β-estradiol (E2, a model of pharmaceutical and personal care products) in water matrix by initial adsorption and subsequent photo-degradation. Characterizations indicated that the modification occurred, i.e., the titanate nanotubes would be grafted onto the activated charcoal (AC) surface, and the micro-carbon could modify the tubular structure of TNTs. E2 was rapidly adsorbed onto TNTs@AC, and the uptake reached 1.87 mg/g from the dual-mode model fitting. Subsequently, the adsorbed E2 could be degraded 99.8% within 2 h under ultraviolet (UV) light irradiation. TNTs@AC was attributed with a unique hybrid structure, providing the hydrophobic effect, π−π interaction, and capillary condensation for E2 adsorption, and facilitating the electron transfer and then enhancing photocatalytic ability for E2-degradation. In addition, the removal mechanism of E2 was elucidated through the density functional theory calculation. Our study is expected to provide a promising material for environmental application.

Effect of Cu2+ ions on the binding of estrogen to the human myometrial estrogen binding protein

Contraception ◽  
1978 ◽  
Vol 18 (2) ◽  
pp. 181-190 ◽  
Author(s):  
Timo Lövgren ◽  
Kim Pettersson ◽  
Bo Lundberg ◽  
Reijo Punnonen
Keyword(s):  
Binding Protein ◽  
Estrogen Binding
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