The estrogen binding protein in human pancreas: Concentrations in subcellular fractions of normal pancreatic tissue, in duodenal juice during pancreatic stimulation and in peripheral serum in normal and pathological conditions

Abstract Background.—The diagnosis of malignancy in pancreatic mucinous cystic tumors depends on demonstrating invasion that may be focal and require extensive sectioning. Objective.—To explore markers that may indicate malignant potential in mucinous cystic tumors. Design.—Routinely processed sections from resected specimens of 12 normal pancreata, 14 pancreata with chronic pancreatitis, 9 mucinous cystic tumors, and 30 invasive adenocarcinomas were immunostained with antibodies to p53, HER-2/neu, epithelial growth factor receptor (EGFR), transforming growth factor α (TGF-α), and Ki-67. Results.—Expression of p53, HER-2/neu, and Ki-67 was significantly more frequent in mucinous tumors than in normal pancreatic tissue and chronic pancreatitis tissue (P = .0003 to .05). Strong expression (more than one third of cells positive) and strong intensity (2+ and 3+) of staining of p53 and EGFR were seen only in carcinomas. Coexpression of p53/HER-2/neu and EGFR/HER-2/neu and a frequency of Ki-67+ nuclei of greater than 5% of cells discriminated between mucinous tumors and normal pancreatic tissue and chronic pancreatitis tissue. p53 expression was significantly more frequent in carcinomas than in mucinous tumor (P = .0326). Coexpression of p53/EGFR discriminated between mucinous tumors and carcinomas; however, TGF-α was not discriminative. Conclusions.—The immunostaining panel of p53, HER-2/neu, Ki-67, and EGFR can be helpful in indicating malignant potential in mucinous tumors of pancreas in routine pathology practice.

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Mercury in Pancreatic Cells of People with and without Pancreatic Cancer

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17238990 ◽

2020 ◽

Vol 17 (23) ◽

pp. 8990

Author(s):

Roger Pamphlett ◽

Andrew J. Colebatch ◽

Philip A. Doble ◽

David P. Bishop

Keyword(s):

Pancreatic Cancer ◽

Toxic Metals ◽

Inductively Coupled Plasma ◽

Islet Cells ◽

Inorganic Mercury ◽

Pancreatic Tissue ◽

Human Pancreas ◽

Inductively Coupled ◽

Pancreatic Cells ◽

Cadmium Lead

Toxic metals have been implicated in the pathogenesis of pancreatic cancer. Human exposure to mercury is widespread, but it is not known how often mercury is present in the human pancreas and which cells might contain mercury. We therefore aimed to determine, in people with and without pancreatic cancer, the distribution and prevalence of mercury in pancreatic cells. Paraffin-embedded sections of normal pancreatic tissue were obtained from pancreatectomy samples of 45 people who had pancreatic adenocarcinoma, and from autopsy samples of 38 people without pancreatic cancer. Mercury was identified using two methods of elemental bio-imaging: (1) With autometallography, inorganic mercury was seen in islet cells in 14 of 30 males (47%) with pancreatic cancer compared to two of 17 males (12%) without pancreatic cancer (p = 0.024), and in 10 of 15 females (67%) with pancreatic cancer compared to four of 21 females (19%) without pancreatic cancer (p = 0.006). Autometallographic mercury was present in acinar cells in 24% and in periductal cells in 11% of people with pancreatic cancer, but not in those without pancreatic cancer. (2) Laser ablation-inductively coupled plasma-mass spectrometry confirmed the presence of mercury in islets that stained with autometallography and detected cadmium, lead, chromium, iron, nickel and aluminium in some samples. In conclusion, the genotoxic metal mercury is found in normal pancreatic cells in more people with, than without, pancreatic cancer. These findings support the hypothesis that toxic metals such as mercury contribute to the pathogenesis of pancreatic cancer.

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Effect of Cu2+ ions on the binding of estrogen to the human myometrial estrogen binding protein

Contraception ◽

10.1016/0010-7824(78)90093-8 ◽

1978 ◽

Vol 18 (2) ◽

pp. 181-190 ◽

Cited By ~ 6

Author(s):

Timo Lövgren ◽

Kim Pettersson ◽

Bo Lundberg ◽

Reijo Punnonen

Keyword(s):

Binding Protein ◽

Estrogen Binding

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The use of reversed-phase high-performance liquid chromatography to detect proteolytic activity from human pancreas in a radioimmunoassay for corticotrophin-releasing factor

Journal of Endocrinology ◽

10.1677/joe.0.1140147 ◽

1987 ◽

Vol 114 (1) ◽

pp. 147-151 ◽

Cited By ~ 13

Author(s):

D. S. Jessop ◽

R. L. Patience ◽

D. Cunnah ◽

L. H. Rees

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Proteolytic Activity ◽

High Performance ◽

Gel Filtration ◽

Reversed Phase ◽

Pancreatic Tissue ◽

Acid Extraction ◽

Human Pancreas ◽

Corticotrophin Releasing Factor

ABSTRACT Degradation of tracer during a radioimmunoassay (RIA) can result in false-positive concentrations of immunoreactivity being reported in a biological sample. A technique has been developed using reversed-phase high-performance liquid chromatography (HPLC) to detect proteolytic degradation of corticotrophin-releasing factor-41 (CRF-41) during incubation with tissue extracts under RIA conditions. Human pancreatic tissue was extracted in HCl or urea and incubated with 125I-labelled CRF-41 at neutral pH for 18 h. When samples were analysed by HPLC and fractions counted for radioactivity, tracer was extensively degraded. Heating extracts at 85 °C or adding lima bean trypsin inhibitor to the medium prevented degradation. Pancreatic tissue extracted in HCl was analysed by gel filtration and HPLC, and fractions were subjected to RIA for CRF-41. A peak of immunoreactivity was detected by both chromatographic methods. However, when this material was incubated with tracer and analysed by HPLC, the tracer was degraded, indicating that proteolytic activity remained after acid extraction and two forms of chromatography. J. Endocr. (1987) 114, 147–151

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A new pituitary protein 7B2 is increased in patients with high α- or β-hCG

Acta Endocrinologica ◽

10.1530/acta.0.1200289 ◽

1989 ◽

Vol 120 (3) ◽

pp. 289-294

Author(s):

U. Leonhardt ◽

B. Eriksson ◽

K. Oberg ◽

L. Wide ◽

M. A. Ghatei ◽

...

Keyword(s):

Amino Acid ◽

Gel Filtration ◽

Pancreatic Tissue ◽

Healthy Controls ◽

Normal Pancreatic Tissue ◽

Amino Acid Peptide ◽

Specific Radioimmunoassay ◽

Gastrointestinal Tumours ◽

Β Hcg ◽

Apud System

Abstract. The presence of 7B2 (a 180 amino acid peptide first extracted from the porcine pituitary) was investigated by a specific radioimmunoassay and gel filtration chromatography in the plasma of three groups of patients known to have increased levels of α- and β-hCG subunits. Plasma 7B2 immunoreactive equivalents (7B2-IE) were increased in postmenopausal women (range 67–143 pmol/l, median: 94 pmol/l, N = 20, P< 0.05), in patients with Klinefelter's syndrome (range 195-230 pmol/l), median 213 pmol/l, N = 4, P< 0.01) and in 17 patients with malignant endocrine gastrointestinal tumours (range 66–20 000 pmol/l, N = 32) compared with healthy controls (range 23.6–98.2 pmol/l, median 41.5 pmol/l, N = 40). Tumour tissue from 4 patients with endocrine pancreatic tumours had significantly higher 7B2-IE concentrations than normal pancreatic tissue, with the highest concentration in an insulinoma (449 pmol/g, normal: 28 pmol/g). During therapy almost parallel changes in plasma 7B2 and other hormones were noted and 7B2-IE therefore might be an additional marker for cancers of the APUD system.

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